ID   ASPG_MACFA              Reviewed;         346 AA.
AC   Q4R6C4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase;
DE            EC=3.5.1.26;
DE   AltName: Full=Glycosylasparaginase;
DE   AltName: Full=Aspartylglucosaminidase;
DE            Short=AGA;
DE   AltName: Full=N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase;
DE   Contains:
DE     RecName: Full=Glycosylasparaginase alpha chain;
DE   Contains:
DE     RecName: Full=Glycosylasparaginase beta chain;
DE   Flags: Precursor;
GN   Name=AGA; ORFNames=QtsA-18326;
OS   Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves the GlcNAc-Asn bond which joins oligosaccharides
CC       to the peptide of asparagine-linked glycoproteins.
CC   -!- CATALYTIC ACTIVITY: N(4)-(beta-N-acetyl-D-glucosaminyl)-L-
CC       asparagine + H(2)O = N-acetyl-beta-D-glucosaminylamine + L-
CC       aspartate.
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta chains arranged
CC       as a dimer of alpha/beta heterodimers (By similarity).
CC   -!- SUBCELLULAR LOCATION: Lysosome (By similarity).
CC   -!- SIMILARITY: Belongs to the Ntn-hydrolase family.
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DR   EMBL; AB169261; BAE01351.1; -; mRNA.
DR   SMR; Q4R6C4; 25-184, 206-346.
DR   MEROPS; T02.001; -.
DR   HOVERGEN; Q4R6C4; -.
DR   BRENDA; 3.5.1.26; 3438.
DR   GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0003948; F:N4-(beta-N-acetylglucosaminyl)-L-asparagina...; IEA:EC.
DR   InterPro; IPR000246; Peptidase_T2.
DR   PANTHER; PTHR10188; Peptidase_T2; 1.
DR   Pfam; PF01112; Asparaginase_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Lysosome; Signal.
FT   SIGNAL        1     23       Potential.
FT   CHAIN        24    205       Glycosylasparaginase alpha chain (By
FT                                similarity).
FT                                /FTId=PRO_0000043405.
FT   CHAIN       206    346       Glycosylasparaginase beta chain (By
FT                                similarity).
FT                                /FTId=PRO_0000043406.
FT   ACT_SITE    206    206       Nucleophile (By similarity).
FT   CARBOHYD     38     38       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    308    308       N-linked (GlcNAc...) (Potential).
FT   DISULFID     64     69       By similarity.
FT   DISULFID    163    179       By similarity.
FT   DISULFID    286    306       By similarity.
FT   DISULFID    317    345       By similarity.
SQ   SEQUENCE   346 AA;  37065 MW;  98174510534A2FB5 CRC64;
     MARKSKLPLL LVPLLLCQAL VRCSSPLPLV LNTWPFKNAT EAAWRALASG GSALDAVESG
     CAMCETEQCG GSVGFGGSPD ELGETTLDAM IMEGTTMDVG AVGDLRRIKN AIGVARKVLE
     HTTHTLLVGE SATKFAESMG FVNEDLSTSA SQALHSDWLA RNCQPNYWRN VVPDPSKYCG
     PYKPLGILKQ DIPIHKETED NRGHDTIGMV VIHKTGRIAA GTSTNGIKFK IHGRVGDSPV
     PGAGAYADDT AGAAAATGEG DILMRFLPSY QAVEYMRGGE DPTIACQKVI SRIQKYFPEF
     FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI
//