Reviewed, UniProtKB/Swiss-Prot Q4R6C4 (ASPG_MACFA)

Names and origin

Protein names

Recommended name:
    N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
    EC=3.5.1.26
Alternative name(s):
    Glycosylasparaginase
    Aspartylglucosaminidase
      Short name=AGA
    N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
Cleaved into the following 2 chains:
    1- Recommended name:
            Glycosylasparaginase alpha chain
    2- Recommended name:
            Glycosylasparaginase beta chain

Gene names

Name:	AGA
ORF Names:	QtsA-18326

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins.
Catalytic activity	N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate.
Subunit structure	Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers By similarity.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 205	182	Glycosylasparaginase alpha chain By similarity		PRO_0000043405
	Chain	206 – 346	141	Glycosylasparaginase beta chain By similarity		PRO_0000043406
Sites
	Active site	206	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Disulfide bond	64 ↔ 69		By similarity
	Disulfide bond	163 ↔ 179		By similarity
	Disulfide bond	286 ↔ 306		By similarity
	Disulfide bond	317 ↔ 345		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R6C4-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 98174510534A2FB5	FASTA	346	37,065
	10 20 30 40 50 60 MARKSKLPLL LVPLLLCQAL VRCSSPLPLV LNTWPFKNAT EAAWRALASG GSALDAVESG 70 80 90 100 110 120 CAMCETEQCG GSVGFGGSPD ELGETTLDAM IMEGTTMDVG AVGDLRRIKN AIGVARKVLE 130 140 150 160 170 180 HTTHTLLVGE SATKFAESMG FVNEDLSTSA SQALHSDWLA RNCQPNYWRN VVPDPSKYCG 190 200 210 220 230 240 PYKPLGILKQ DIPIHKETED NRGHDTIGMV VIHKTGRIAA GTSTNGIKFK IHGRVGDSPV 250 260 270 280 290 300 PGAGAYADDT AGAAAATGEG DILMRFLPSY QAVEYMRGGE DPTIACQKVI SRIQKYFPEF 310 320 330 340 FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169261 mRNA. Translation: BAE01351.1.
3D structure databases
SMR	Q4R6C4. Positions 25-184, 206-346.
ModBase	Search...
Protein family/group databases
MEROPS	T02.001.
Phylogenomic databases
HOVERGEN	Q4R6C4.
Enzyme and pathway databases
BRENDA	3.5.1.26. 3438.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_MACFA

Accession

Primary (citable) accession number: Q4R6C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)