ID   SYIM_MACFA              Reviewed;         993 AA.
AC   Q4R646;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 2.
DT   16-JUN-2009, entry version 34.
DE   RecName: Full=Isoleucyl-tRNA synthetase, mitochondrial;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucine--tRNA ligase;
DE            Short=IleRS;
DE   Flags: Precursor; Fragment;
GN   Name=IARS2; ORFNames=QtsA-19161;
OS   Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-isoleucine + tRNA(Ile) = AMP +
CC       diphosphate + L-isoleucyl-tRNA(Ile).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; AB169344; BAE01429.1; ALT_INIT; mRNA.
DR   HOVERGEN; Q4R646; -.
DR   BRENDA; 6.1.1.5; 3438.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR010663; DNA_glyclase/IsotRNA_synth_Znf.
DR   InterPro; IPR002301; Ile-tRNA-synt_Ia.
DR   InterPro; IPR015905; Ile-tRNA-synt_Ia_N.
DR   InterPro; IPR018353; Isoleucyl-tRNA_synthetase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR013155; V/L/I-tRNA-synth_anticodon-bd.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11946:SF9; Ile-tRNA-synt_Ia; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF06827; zf-FPG_IleRS; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   TIGRFAMs; TIGR00392; ileS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Phosphoprotein; Protein biosynthesis;
KW   Transit peptide.
FT   TRANSIT      <1     29       Mitochondrion (Potential).
FT   CHAIN        30    993       Isoleucyl-tRNA synthetase, mitochondrial.
FT                                /FTId=PRO_0000233336.
FT   MOTIF        97    107       "HIGH" region (By similarity).
FT   MOTIF       645    649       "KMSKS" region (By similarity).
FT   BINDING     648    648       ATP (By similarity).
FT   MOD_RES     167    167       Phosphoserine (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   993 AA;  111941 MW;  E8C8F0C7458F0AD2 CRC64;
     SLWGTPRLPC SPGWQGATKR LLVRSVSGAS NHQPNSNTGR YRDTVLLPQT SFPMKLLGRQ
     QPDKELEIQQ KCGFSELYSW QRERKVKTEF CLHDGPPYAN GDPHVGHALN KILKDIANRF
     HMMSGSKVHF VPGWDCHGLP IEIKVLSELG REAQNLSAME IREKARSFAK AAIEKQKSAF
     IRWGIMADWN NCYYTFDGKY EAKQLRTFYQ MYDKGLVYRS YKPVFWSPSS RTALAEAELE
     YNPEHVSRSI YVKFPLLKPS PKLASLIDGS SPVSFLVWTT QPWTIPANEA VCYMPESKYA
     VVKCSKSGDL YVLAADKVET VASTLETAFE TISTFSGVDL ENGTCSHPLI PDKASPLLPA
     NHVTMAKGTG LVHTAPAHGM EDYGVASQHN LPMDCLVDED GVFTDVAGPE LQNKAVLEEG
     TDVVIKMLQT AKNLLKEEKL VHSYPYDWRT KKPVVIRASK QWFINIADIK IAAKELLKKV
     KFIPGSALNG MVEMMDRRPY WCISRQRVWG VPIPVFHHKT KDEYLINSQT IEHIVKLVEQ
     HGSDVWWTLP PEQLLPKEVL SEVGGPDALE YVPGQDILDI WFDSGTSWSH VLPGPDQRAD
     LYLEGKDQLG GWFQSSLLTS VATRKKAPYK TVIVHGFTLG EKGEKMSKSL GNVIHPDVVV
     NGGQDQSKEP PYGADVLRWW VADSNVFTEV AIGPSVLNAA RDDISKLRNT LRFLLGNVAD
     FNPETDSIPV NDMYVIDQYM LHLLQDLANK ITELYKQYDF GKVVRLLRTF YTRELSHFYF
     SIIKDRLYCE KENDPRRRSC QTALVEILDV IVRSFAPILP HLAEEVFQHI PYIKEPKSVF
     RTGWISTSSI WKKPGLEEAV ESVCAMRDSF LGSIPGKNAA EYKVIIVIEP GLLFEIIEML
     QSEETSSTSQ LNELMMASES TLLAQEPREL TADVIELKGK FLINLEGGDI REESSYKVIV
     MPTTKEKCPR CWKYTAESSD TLCPRCAEVV SGK
//