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Q4R646

- SYIM_MACFA

UniProt

Q4R646 - SYIM_MACFA

Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

IARS2

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 2 (02 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei648 – 6481ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:IARS2
    ORF Names:QtsA-19161
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei‹1 – 29›29MitochondrionSequence AnalysisAdd
    BLAST
    Chaini30 – 993964Isoleucine--tRNA ligase, mitochondrialPRO_0000233336Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551N6-acetyllysine; alternateBy similarity
    Modified residuei55 – 551N6-succinyllysine; alternateBy similarity
    Modified residuei170 – 1701N6-acetyllysineBy similarity
    Modified residuei175 – 1751N6-succinyllysineBy similarity
    Modified residuei214 – 2141N6-acetyllysineBy similarity
    Modified residuei222 – 2221N6-acetyllysine; alternateBy similarity
    Modified residuei222 – 2221N6-succinyllysine; alternateBy similarity
    Modified residuei460 – 4601N6-succinyllysineBy similarity
    Modified residuei481 – 4811N6-succinyllysineBy similarity
    Modified residuei706 – 7061N6-acetyllysineBy similarity
    Modified residuei756 – 7561N6-acetyllysine; alternateBy similarity
    Modified residuei756 – 7561N6-succinyllysine; alternateBy similarity
    Modified residuei762 – 7621N6-acetyllysine; alternateBy similarity
    Modified residuei762 – 7621N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ4R646.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4R646.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi97 – 10711"HIGH" regionBy similarityAdd
    BLAST
    Motifi645 – 6495"KMSKS" regionBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG059862.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4R646-1 [UniParc]FASTAAdd to Basket

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    SLWGTPRLPC SPGWQGATKR LLVRSVSGAS NHQPNSNTGR YRDTVLLPQT    50
    SFPMKLLGRQ QPDKELEIQQ KCGFSELYSW QRERKVKTEF CLHDGPPYAN 100
    GDPHVGHALN KILKDIANRF HMMSGSKVHF VPGWDCHGLP IEIKVLSELG 150
    REAQNLSAME IREKARSFAK AAIEKQKSAF IRWGIMADWN NCYYTFDGKY 200
    EAKQLRTFYQ MYDKGLVYRS YKPVFWSPSS RTALAEAELE YNPEHVSRSI 250
    YVKFPLLKPS PKLASLIDGS SPVSFLVWTT QPWTIPANEA VCYMPESKYA 300
    VVKCSKSGDL YVLAADKVET VASTLETAFE TISTFSGVDL ENGTCSHPLI 350
    PDKASPLLPA NHVTMAKGTG LVHTAPAHGM EDYGVASQHN LPMDCLVDED 400
    GVFTDVAGPE LQNKAVLEEG TDVVIKMLQT AKNLLKEEKL VHSYPYDWRT 450
    KKPVVIRASK QWFINIADIK IAAKELLKKV KFIPGSALNG MVEMMDRRPY 500
    WCISRQRVWG VPIPVFHHKT KDEYLINSQT IEHIVKLVEQ HGSDVWWTLP 550
    PEQLLPKEVL SEVGGPDALE YVPGQDILDI WFDSGTSWSH VLPGPDQRAD 600
    LYLEGKDQLG GWFQSSLLTS VATRKKAPYK TVIVHGFTLG EKGEKMSKSL 650
    GNVIHPDVVV NGGQDQSKEP PYGADVLRWW VADSNVFTEV AIGPSVLNAA 700
    RDDISKLRNT LRFLLGNVAD FNPETDSIPV NDMYVIDQYM LHLLQDLANK 750
    ITELYKQYDF GKVVRLLRTF YTRELSHFYF SIIKDRLYCE KENDPRRRSC 800
    QTALVEILDV IVRSFAPILP HLAEEVFQHI PYIKEPKSVF RTGWISTSSI 850
    WKKPGLEEAV ESVCAMRDSF LGSIPGKNAA EYKVIIVIEP GLLFEIIEML 900
    QSEETSSTSQ LNELMMASES TLLAQEPREL TADVIELKGK FLINLEGGDI 950
    REESSYKVIV MPTTKEKCPR CWKYTAESSD TLCPRCAEVV SGK 993
    Length:993
    Mass (Da):111,941
    Last modified:May 2, 2006 - v2
    Checksum:iE8C8F0C7458F0AD2
    GO

    Sequence cautioni

    The sequence BAE01429.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169344 mRNA. Translation: BAE01429.1. Different initiation.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169344 mRNA. Translation: BAE01429.1 . Different initiation.

    3D structure databases

    ProteinModelPortali Q4R646.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q4R646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG059862.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiSYIM_MACFA
    AccessioniPrimary (citable) accession number: Q4R646
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3