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Q4R646 (SYIM_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, mitochondrial

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:IARS2
ORF Names:QtsA-19161
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length993 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Subcellular location

Mitochondrion matrix By similarity HAMAP-Rule MF_02002.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence BAE01429.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   PTMAcetylation
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 29›29Mitochondrion Potential
Chain30 – 993964Isoleucine--tRNA ligase, mitochondrial HAMAP-Rule MF_02002
PRO_0000233336

Regions

Motif97 – 10711"HIGH" region By similarity
Motif645 – 6495"KMSKS" region By similarity

Sites

Binding site6481ATP By similarity

Amino acid modifications

Modified residue551N6-acetyllysine; alternate By similarity
Modified residue551N6-succinyllysine; alternate By similarity
Modified residue1701N6-acetyllysine By similarity
Modified residue1751N6-succinyllysine By similarity
Modified residue2141N6-acetyllysine By similarity
Modified residue2221N6-acetyllysine; alternate By similarity
Modified residue2221N6-succinyllysine; alternate By similarity
Modified residue4601N6-succinyllysine By similarity
Modified residue4811N6-succinyllysine By similarity
Modified residue7061N6-acetyllysine By similarity
Modified residue7561N6-acetyllysine; alternate By similarity
Modified residue7561N6-succinyllysine; alternate By similarity
Modified residue7621N6-acetyllysine; alternate By similarity
Modified residue7621N6-succinyllysine; alternate By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q4R646 [UniParc].

Last modified May 2, 2006. Version 2.
Checksum: E8C8F0C7458F0AD2

FASTA993111,941
        10         20         30         40         50         60 
SLWGTPRLPC SPGWQGATKR LLVRSVSGAS NHQPNSNTGR YRDTVLLPQT SFPMKLLGRQ 

        70         80         90        100        110        120 
QPDKELEIQQ KCGFSELYSW QRERKVKTEF CLHDGPPYAN GDPHVGHALN KILKDIANRF 

       130        140        150        160        170        180 
HMMSGSKVHF VPGWDCHGLP IEIKVLSELG REAQNLSAME IREKARSFAK AAIEKQKSAF 

       190        200        210        220        230        240 
IRWGIMADWN NCYYTFDGKY EAKQLRTFYQ MYDKGLVYRS YKPVFWSPSS RTALAEAELE 

       250        260        270        280        290        300 
YNPEHVSRSI YVKFPLLKPS PKLASLIDGS SPVSFLVWTT QPWTIPANEA VCYMPESKYA 

       310        320        330        340        350        360 
VVKCSKSGDL YVLAADKVET VASTLETAFE TISTFSGVDL ENGTCSHPLI PDKASPLLPA 

       370        380        390        400        410        420 
NHVTMAKGTG LVHTAPAHGM EDYGVASQHN LPMDCLVDED GVFTDVAGPE LQNKAVLEEG 

       430        440        450        460        470        480 
TDVVIKMLQT AKNLLKEEKL VHSYPYDWRT KKPVVIRASK QWFINIADIK IAAKELLKKV 

       490        500        510        520        530        540 
KFIPGSALNG MVEMMDRRPY WCISRQRVWG VPIPVFHHKT KDEYLINSQT IEHIVKLVEQ 

       550        560        570        580        590        600 
HGSDVWWTLP PEQLLPKEVL SEVGGPDALE YVPGQDILDI WFDSGTSWSH VLPGPDQRAD 

       610        620        630        640        650        660 
LYLEGKDQLG GWFQSSLLTS VATRKKAPYK TVIVHGFTLG EKGEKMSKSL GNVIHPDVVV 

       670        680        690        700        710        720 
NGGQDQSKEP PYGADVLRWW VADSNVFTEV AIGPSVLNAA RDDISKLRNT LRFLLGNVAD 

       730        740        750        760        770        780 
FNPETDSIPV NDMYVIDQYM LHLLQDLANK ITELYKQYDF GKVVRLLRTF YTRELSHFYF 

       790        800        810        820        830        840 
SIIKDRLYCE KENDPRRRSC QTALVEILDV IVRSFAPILP HLAEEVFQHI PYIKEPKSVF 

       850        860        870        880        890        900 
RTGWISTSSI WKKPGLEEAV ESVCAMRDSF LGSIPGKNAA EYKVIIVIEP GLLFEIIEML 

       910        920        930        940        950        960 
QSEETSSTSQ LNELMMASES TLLAQEPREL TADVIELKGK FLINLEGGDI REESSYKVIV 

       970        980        990 
MPTTKEKCPR CWKYTAESSD TLCPRCAEVV SGK 

« Hide

References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB169344 mRNA. Translation: BAE01429.1. Different initiation.

3D structure databases

ProteinModelPortalQ4R646.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ4R646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG059862.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYIM_MACFA
AccessionPrimary (citable) accession number: Q4R646
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries