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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

IARS2

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei648 – 6481ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:IARS2
ORF Names:QtsA-19161
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 29›29MitochondrionSequence AnalysisAdd
BLAST
Chaini30 – 993964Isoleucine--tRNA ligase, mitochondrialPRO_0000233336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551N6-acetyllysine; alternateBy similarity
Modified residuei55 – 551N6-succinyllysine; alternateBy similarity
Modified residuei170 – 1701N6-acetyllysineBy similarity
Modified residuei175 – 1751N6-succinyllysineBy similarity
Modified residuei214 – 2141N6-acetyllysineBy similarity
Modified residuei222 – 2221N6-acetyllysine; alternateBy similarity
Modified residuei222 – 2221N6-succinyllysine; alternateBy similarity
Modified residuei460 – 4601N6-succinyllysineBy similarity
Modified residuei481 – 4811N6-succinyllysineBy similarity
Modified residuei706 – 7061N6-acetyllysineBy similarity
Modified residuei756 – 7561N6-acetyllysine; alternateBy similarity
Modified residuei756 – 7561N6-succinyllysine; alternateBy similarity
Modified residuei762 – 7621N6-acetyllysine; alternateBy similarity
Modified residuei762 – 7621N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ4R646.

Structurei

3D structure databases

ProteinModelPortaliQ4R646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi97 – 10711"HIGH" regionBy similarityAdd
BLAST
Motifi645 – 6495"KMSKS" regionBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG059862.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SLWGTPRLPC SPGWQGATKR LLVRSVSGAS NHQPNSNTGR YRDTVLLPQT
60 70 80 90 100
SFPMKLLGRQ QPDKELEIQQ KCGFSELYSW QRERKVKTEF CLHDGPPYAN
110 120 130 140 150
GDPHVGHALN KILKDIANRF HMMSGSKVHF VPGWDCHGLP IEIKVLSELG
160 170 180 190 200
REAQNLSAME IREKARSFAK AAIEKQKSAF IRWGIMADWN NCYYTFDGKY
210 220 230 240 250
EAKQLRTFYQ MYDKGLVYRS YKPVFWSPSS RTALAEAELE YNPEHVSRSI
260 270 280 290 300
YVKFPLLKPS PKLASLIDGS SPVSFLVWTT QPWTIPANEA VCYMPESKYA
310 320 330 340 350
VVKCSKSGDL YVLAADKVET VASTLETAFE TISTFSGVDL ENGTCSHPLI
360 370 380 390 400
PDKASPLLPA NHVTMAKGTG LVHTAPAHGM EDYGVASQHN LPMDCLVDED
410 420 430 440 450
GVFTDVAGPE LQNKAVLEEG TDVVIKMLQT AKNLLKEEKL VHSYPYDWRT
460 470 480 490 500
KKPVVIRASK QWFINIADIK IAAKELLKKV KFIPGSALNG MVEMMDRRPY
510 520 530 540 550
WCISRQRVWG VPIPVFHHKT KDEYLINSQT IEHIVKLVEQ HGSDVWWTLP
560 570 580 590 600
PEQLLPKEVL SEVGGPDALE YVPGQDILDI WFDSGTSWSH VLPGPDQRAD
610 620 630 640 650
LYLEGKDQLG GWFQSSLLTS VATRKKAPYK TVIVHGFTLG EKGEKMSKSL
660 670 680 690 700
GNVIHPDVVV NGGQDQSKEP PYGADVLRWW VADSNVFTEV AIGPSVLNAA
710 720 730 740 750
RDDISKLRNT LRFLLGNVAD FNPETDSIPV NDMYVIDQYM LHLLQDLANK
760 770 780 790 800
ITELYKQYDF GKVVRLLRTF YTRELSHFYF SIIKDRLYCE KENDPRRRSC
810 820 830 840 850
QTALVEILDV IVRSFAPILP HLAEEVFQHI PYIKEPKSVF RTGWISTSSI
860 870 880 890 900
WKKPGLEEAV ESVCAMRDSF LGSIPGKNAA EYKVIIVIEP GLLFEIIEML
910 920 930 940 950
QSEETSSTSQ LNELMMASES TLLAQEPREL TADVIELKGK FLINLEGGDI
960 970 980 990
REESSYKVIV MPTTKEKCPR CWKYTAESSD TLCPRCAEVV SGK
Length:993
Mass (Da):111,941
Last modified:May 2, 2006 - v2
Checksum:iE8C8F0C7458F0AD2
GO

Sequence cautioni

The sequence BAE01429.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169344 mRNA. Translation: BAE01429.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169344 mRNA. Translation: BAE01429.1. Different initiation.

3D structure databases

ProteinModelPortaliQ4R646.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ4R646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG059862.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiSYIM_MACFA
AccessioniPrimary (citable) accession number: Q4R646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: March 4, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.