Q4R646 (SYIM_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase, mitochondrial
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	IARS2
ORF Names:	QtsA-19161

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	993 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAE01429.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 29	›29	Mitochondrion Potential
	Chain	30 – 993	964	Isoleucine--tRNA ligase, mitochondrial		PRO_0000233336
Regions
	Motif	97 – 107	11	"HIGH" region By similarity
	Motif	645 – 649	5	"KMSKS" region By similarity
Sites
	Binding site	648	1	ATP By similarity
Amino acid modifications
	Modified residue	170	1	N6-acetyllysine By similarity
	Modified residue	214	1	N6-acetyllysine By similarity
	Modified residue	222	1	N6-acetyllysine By similarity
	Modified residue	756	1	N6-acetyllysine By similarity
	Modified residue	762	1	N6-acetyllysine By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q4R646 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: E8C8F0C7458F0AD2	FASTA	993	111,941
	10 20 30 40 50 60 SLWGTPRLPC SPGWQGATKR LLVRSVSGAS NHQPNSNTGR YRDTVLLPQT SFPMKLLGRQ 70 80 90 100 110 120 QPDKELEIQQ KCGFSELYSW QRERKVKTEF CLHDGPPYAN GDPHVGHALN KILKDIANRF 130 140 150 160 170 180 HMMSGSKVHF VPGWDCHGLP IEIKVLSELG REAQNLSAME IREKARSFAK AAIEKQKSAF 190 200 210 220 230 240 IRWGIMADWN NCYYTFDGKY EAKQLRTFYQ MYDKGLVYRS YKPVFWSPSS RTALAEAELE 250 260 270 280 290 300 YNPEHVSRSI YVKFPLLKPS PKLASLIDGS SPVSFLVWTT QPWTIPANEA VCYMPESKYA 310 320 330 340 350 360 VVKCSKSGDL YVLAADKVET VASTLETAFE TISTFSGVDL ENGTCSHPLI PDKASPLLPA 370 380 390 400 410 420 NHVTMAKGTG LVHTAPAHGM EDYGVASQHN LPMDCLVDED GVFTDVAGPE LQNKAVLEEG 430 440 450 460 470 480 TDVVIKMLQT AKNLLKEEKL VHSYPYDWRT KKPVVIRASK QWFINIADIK IAAKELLKKV 490 500 510 520 530 540 KFIPGSALNG MVEMMDRRPY WCISRQRVWG VPIPVFHHKT KDEYLINSQT IEHIVKLVEQ 550 560 570 580 590 600 HGSDVWWTLP PEQLLPKEVL SEVGGPDALE YVPGQDILDI WFDSGTSWSH VLPGPDQRAD 610 620 630 640 650 660 LYLEGKDQLG GWFQSSLLTS VATRKKAPYK TVIVHGFTLG EKGEKMSKSL GNVIHPDVVV 670 680 690 700 710 720 NGGQDQSKEP PYGADVLRWW VADSNVFTEV AIGPSVLNAA RDDISKLRNT LRFLLGNVAD 730 740 750 760 770 780 FNPETDSIPV NDMYVIDQYM LHLLQDLANK ITELYKQYDF GKVVRLLRTF YTRELSHFYF 790 800 810 820 830 840 SIIKDRLYCE KENDPRRRSC QTALVEILDV IVRSFAPILP HLAEEVFQHI PYIKEPKSVF 850 860 870 880 890 900 RTGWISTSSI WKKPGLEEAV ESVCAMRDSF LGSIPGKNAA EYKVIIVIEP GLLFEIIEML 910 920 930 940 950 960 QSEETSSTSQ LNELMMASES TLLAQEPREL TADVIELKGK FLINLEGGDI REESSYKVIV 970 980 990 MPTTKEKCPR CWKYTAESSD TLCPRCAEVV SGK

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169344 mRNA. Translation: BAE01429.1. Different initiation.
3D structure databases
ProteinModelPortal	Q4R646.
ModBase	Search...
Proteomic databases
PRIDE	Q4R646.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG059862.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIM_MACFA

Accession

Primary (citable) accession number: Q4R646

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	March 6, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program