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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

IARS2

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei648ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:IARS2
ORF Names:QtsA-19161
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei‹1 – 29MitochondrionSequence analysisAdd BLAST›29
ChainiPRO_000023333630 – 993Isoleucine--tRNA ligase, mitochondrialAdd BLAST964

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55N6-acetyllysine; alternateBy similarity1
Modified residuei55N6-succinyllysine; alternateBy similarity1
Modified residuei170N6-acetyllysineBy similarity1
Modified residuei175N6-succinyllysineBy similarity1
Modified residuei214N6-acetyllysineBy similarity1
Modified residuei222N6-acetyllysine; alternateBy similarity1
Modified residuei222N6-succinyllysine; alternateBy similarity1
Modified residuei460N6-succinyllysineBy similarity1
Modified residuei481N6-succinyllysineBy similarity1
Modified residuei706N6-acetyllysineBy similarity1
Modified residuei756N6-acetyllysine; alternateBy similarity1
Modified residuei756N6-succinyllysine; alternateBy similarity1
Modified residuei762N6-acetyllysine; alternateBy similarity1
Modified residuei762N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ4R646.

Structurei

3D structure databases

ProteinModelPortaliQ4R646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 107"HIGH" regionBy similarityAdd BLAST11
Motifi645 – 649"KMSKS" regionBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG059862.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SLWGTPRLPC SPGWQGATKR LLVRSVSGAS NHQPNSNTGR YRDTVLLPQT
60 70 80 90 100
SFPMKLLGRQ QPDKELEIQQ KCGFSELYSW QRERKVKTEF CLHDGPPYAN
110 120 130 140 150
GDPHVGHALN KILKDIANRF HMMSGSKVHF VPGWDCHGLP IEIKVLSELG
160 170 180 190 200
REAQNLSAME IREKARSFAK AAIEKQKSAF IRWGIMADWN NCYYTFDGKY
210 220 230 240 250
EAKQLRTFYQ MYDKGLVYRS YKPVFWSPSS RTALAEAELE YNPEHVSRSI
260 270 280 290 300
YVKFPLLKPS PKLASLIDGS SPVSFLVWTT QPWTIPANEA VCYMPESKYA
310 320 330 340 350
VVKCSKSGDL YVLAADKVET VASTLETAFE TISTFSGVDL ENGTCSHPLI
360 370 380 390 400
PDKASPLLPA NHVTMAKGTG LVHTAPAHGM EDYGVASQHN LPMDCLVDED
410 420 430 440 450
GVFTDVAGPE LQNKAVLEEG TDVVIKMLQT AKNLLKEEKL VHSYPYDWRT
460 470 480 490 500
KKPVVIRASK QWFINIADIK IAAKELLKKV KFIPGSALNG MVEMMDRRPY
510 520 530 540 550
WCISRQRVWG VPIPVFHHKT KDEYLINSQT IEHIVKLVEQ HGSDVWWTLP
560 570 580 590 600
PEQLLPKEVL SEVGGPDALE YVPGQDILDI WFDSGTSWSH VLPGPDQRAD
610 620 630 640 650
LYLEGKDQLG GWFQSSLLTS VATRKKAPYK TVIVHGFTLG EKGEKMSKSL
660 670 680 690 700
GNVIHPDVVV NGGQDQSKEP PYGADVLRWW VADSNVFTEV AIGPSVLNAA
710 720 730 740 750
RDDISKLRNT LRFLLGNVAD FNPETDSIPV NDMYVIDQYM LHLLQDLANK
760 770 780 790 800
ITELYKQYDF GKVVRLLRTF YTRELSHFYF SIIKDRLYCE KENDPRRRSC
810 820 830 840 850
QTALVEILDV IVRSFAPILP HLAEEVFQHI PYIKEPKSVF RTGWISTSSI
860 870 880 890 900
WKKPGLEEAV ESVCAMRDSF LGSIPGKNAA EYKVIIVIEP GLLFEIIEML
910 920 930 940 950
QSEETSSTSQ LNELMMASES TLLAQEPREL TADVIELKGK FLINLEGGDI
960 970 980 990
REESSYKVIV MPTTKEKCPR CWKYTAESSD TLCPRCAEVV SGK
Length:993
Mass (Da):111,941
Last modified:May 2, 2006 - v2
Checksum:iE8C8F0C7458F0AD2
GO

Sequence cautioni

The sequence BAE01429 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169344 mRNA. Translation: BAE01429.1. Different initiation.
UniGeneiMfa.7111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169344 mRNA. Translation: BAE01429.1. Different initiation.
UniGeneiMfa.7111.

3D structure databases

ProteinModelPortaliQ4R646.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ4R646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG059862.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYIM_MACFA
AccessioniPrimary (citable) accession number: Q4R646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: October 5, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.