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Q4R5Y2 (NADE_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine-dependent NAD(+) synthetase

EC=6.3.5.1
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
NAD(+) synthetase
Gene names
Name:NADSYN1
ORF Names:QtsA-19858
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.

Subunit structure

Homohexamer By similarity.

Sequence similarities

In the C-terminal section; belongs to the NAD synthetase family.

Contains 1 CN hydrolase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Glutamine-dependent NAD(+) synthetase
PRO_0000237578

Regions

Domain5 – 299295CN hydrolase
Nucleotide binding355 – 3628ATP By similarity
Region325 – 706382Ligase By similarity

Sites

Active site3571 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4R5Y2 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 1B3AF9BF3EF0523B

FASTA70679,071
        10         20         30         40         50         60 
MGRKVTVATC ALNQWALDFE GNLQRILKSI EIAKNRGARY RLGPELEICG YGCWDHYYES 

        70         80         90        100        110        120 
DTLLHSFQVL AALLESPVTQ DIICDVGMPV MHRNVRYNCR VIFLSRKILL IRPKMALANE 

       130        140        150        160        170        180 
GNYRELRWFT PWSRSRHTEE YLLPRMIQDL TKQETAPFGD AVLATWDTCI GSEICEELWT 

       190        200        210        220        230        240 
PHSPHIDMGL DGVEIITNAS GSHHVLRKAN TRVDLVTMAT SKNGGIYLLA NQKGCDGDRL 

       250        260        270        280        290        300 
YYDGCAMIAM NGSVFAQGSQ FSLDDVEVLT ATLDLEDVRS YRAEISSRNL AASRASPYPR 

       310        320        330        340        350        360 
VKVDFALSCH EDLLAPVSEP IEWKYHSPEE EISLGPACWL WDFLRRSQQG GFLLPLSGGV 

       370        380        390        400        410        420 
DSAATACLVY SMCCQVCKSV RSGNQEVLAD VRTIVNQISY TPQDPRDLCG HILTTCYMAS 

       430        440        450        460        470        480 
KNSSQETCTR ARELAQQIGS HHISLNIDPA VKAVTGIFSL VTGKSPLFAA HGGSSRENLA 

       490        500        510        520        530        540 
LQNVQARIRM VLAYLFAQLS LWSRGIRGGL LVLGSANVDE SLLGYLTKYD CSSADINPIG 

       550        560        570        580        590        600 
GISKTDLRAF VQFCIERFQL TALQSIISAP VTAELEPLAD GQVSQTDEED MGMTYAELSV 

       610        620        630        640        650        660 
YGKLRKVAKM GPYSMFCKLL GMWRHVCTPR QVADKVKWFF TKHSMNRHKM TTLTPAYHAE 

       670        680        690        700 
NYSPEDNRFD LRPFLYNTSW PWQFRCIENQ VLQLERAAPQ SLDGVD 

« Hide

References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB169410 mRNA. Translation: BAE01493.1.
RefSeqNP_001270170.1. NM_001283241.1.
UniGeneMfa.7139.

3D structure databases

ProteinModelPortalQ4R5Y2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101926168.
KEGGmcf:101926168.

Organism-specific databases

CTD55191.

Phylogenomic databases

HOVERGENHBG082007.
KOK01950.

Enzyme and pathway databases

UniPathwayUPA00253; UER00334.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF006630. NADS_GAT. 1 hit.
SUPFAMSSF56317. SSF56317. 1 hit.
TIGRFAMsTIGR00552. nadE. 1 hit.
PROSITEPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNADE_MACFA
AccessionPrimary (citable) accession number: Q4R5Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways