Q4R5X9 (RAD9A_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell cycle checkpoint control protein RAD9A
EC=3.1.11.2

Alternative name(s):
DNA repair exonuclease rad9 homolog A

Gene names

Name:	RAD9A
ORF Names:	QtsA-19913

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHINO to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity By similarity.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subunit structure	Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHINO By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex By similarity.
Sequence similarities	Belongs to the rad9 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Cell cycle checkpoint control protein RAD9A		PRO_0000225002
Regions
	Region	40 – 80	41	Possesses 3'-5' exonuclease activity By similarity
	Region	255 – 379	125	Sufficient for interaction with ABL1 By similarity
Amino acid modifications
	Modified residue	17	1	Phosphotyrosine By similarity
	Modified residue	261	1	Phosphoserine By similarity
	Modified residue	266	1	Phosphoserine By similarity
	Modified residue	317	1	Phosphoserine By similarity
	Modified residue	330	1	Phosphoserine By similarity
	Modified residue	363	1	Phosphoserine By similarity
	Modified residue	368	1	Phosphoserine By similarity
	Modified residue	375	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R5X9 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: E49C1EE09FAA1576	FASTA	379	41,370
	10 20 30 40 50 60 MLGKAVHSLS RIGDELYLEP LEDGLSLRTV NSSRSAYACF LFAPLFFQQY QAATPGQDLL 70 80 90 100 110 120 RCKILMKSFL SVFRSLAMLE KTVEKCCISL NGRSSRLVVQ LHCKFGVRKT HNLSFQDCES 130 140 150 160 170 180 LQAVFDPASC PHMLRAPARV LGEAVLPFPP ALAEVTLGIG RGRRVILRSY HEEEADSTAK 190 200 210 220 230 240 AMVTEMCLGE EDFQQLQAQE GVAITFCLKE FRGLLSFAES ANLNLSIHFD APGRPAIFTI 250 260 270 280 290 300 KDSLLDGHFV LATLSDTDSH SQDLGSPERH QPVPQLQAHS IPHPDDFAND DIDSYMIAME 310 320 330 340 350 360 TTIGNEGSRV LPSISLSPGP QHPESPGLHS EEDEAEPSTV PGTPPPKKFR SLFFGSILAP 370 ARSPQGPSPV LAEDSEGEG

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169413 mRNA. Translation: BAE01496.1.
3D structure databases
ProteinModelPortal	Q4R5X9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG058989.
Family and domain databases
InterPro	IPR007268. Rad9. [Graphical view]
PANTHER	PTHR15237. Rad9. 1 hit.
Pfam	PF04139. Rad9. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAD9A_MACFA

Accession

Primary (citable) accession number: Q4R5X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2005
Last modified:	September 21, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program