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Q4R5N9 (PPAL_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal acid phosphatase

Short name=LAP
EC=3.1.3.2
Gene names
Name:ACP2
ORF Names:QccE-11805
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.

Post-translational modification

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.

Sequence similarities

Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
   Cellular componentLysosome
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosomal lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 By similarity
Chain31 – 423393Lysosomal acid phosphatase
PRO_0000352521

Regions

Topological domain31 – 380350Lumenal Potential
Transmembrane381 – 40121Helical; Potential
Topological domain402 – 42322Cytoplasmic Potential

Sites

Active site421Nucleophile By similarity
Active site2871Proton donor By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation1911N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Disulfide bond159 ↔ 370 By similarity
Disulfide bond212 ↔ 310 By similarity
Disulfide bond345 ↔ 349 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4R5N9 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 6835A18779B7B9B7

FASTA42348,310
        10         20         30         40         50         60 
MAGKRSGWSR AALLQLLLGA NLMVMPPTQA RSLRFVTLLY RHGDRSPVKT YPKDPYQEEE 

        70         80         90        100        110        120 
WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV YVRSTDFDRT LMSAEANLAG 

       130        140        150        160        170        180 
LFPPNGMQRF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR KTPEYQNESS 

       190        200        210        220        230        240 
RNAQFLDMVA NETGLTDLTL ETVWNVYDTL FCEQTHGLHL PPWASPQTMQ RLSRLKDFSF 

       250        260        270        280        290        300 
RFLFGIYQQA EKARLQGGVL LAQIRRNLTL MATTSQLPKL LVYSAHDTTL VALQMALDVY 

       310        320        330        340        350        360 
NGEQAPYASC HIFELYQEDS GNFSVEMYFR NESDKAPWPL SLPGCPHRCP LQDFLHLTEP 

       370        380        390        400        410        420 
VVPKDWQQEC QLASGPADTE VIVALAVCGS ILFLLIVLLL TVLFRMQAQP PGYRHVADGE 


DHA 

« Hide

References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB169504 mRNA. Translation: BAE01586.1.
RefSeqXP_005578051.1. XM_005577994.1.
UniGeneMfa.8272.

3D structure databases

ProteinModelPortalQ4R5N9.
SMRQ4R5N9. Positions 31-371.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ4R5N9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101864829.
KEGGmcf:101864829.

Organism-specific databases

CTD53.

Phylogenomic databases

HOVERGENHBG002203.
KOK14410.

Family and domain databases

InterProIPR000560. His_Pase_superF_clade-2.
[Graphical view]
PfamPF00328. His_Phos_2. 1 hit.
[Graphical view]
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPAL_MACFA
AccessionPrimary (citable) accession number: Q4R5N9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families