Q4R5N4 (UB2D3_MACFA) Reviewed, UniProtKB/Swiss-Prot
Last modified October 16, 2013. Version 50. History...
Names and origin
|Protein names||Recommended name:|
Ubiquitin-conjugating enzyme E2 D3
Ubiquitin carrier protein D3
Ubiquitin-protein ligase D3
|Organism||Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)|
|Taxonomic identifier||9541 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca|
|Sequence length||147 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction By similarity.
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex; when Cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened By similarity. Interacts with DAPK3 By similarity. Interacts with BRCA1; the DNA damage checkpoint promotes the association with BRCA1 after ionizing radiation By similarity. Interacts non-covalently with ubiquitin By similarity.
Belongs to the ubiquitin-conjugating enzyme family.
Sequence annotation (Features)
|||"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."|
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.
|AB169509 mRNA. Translation: BAE01591.1.|
3D structure databases
|SMR||Q4R5N4. Positions 1-147. |
Protocols and materials databases
Enzyme and pathway databases
Family and domain databases
|Gene3D||188.8.131.52. 1 hit. |
|InterPro||IPR000608. UBQ-conjugat_E2. |
|Pfam||PF00179. UQ_con. 1 hit. |
|SUPFAM||SSF54495. SSF54495. 1 hit. |
|PROSITE||PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. |
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
|Accession||Primary (citable) accession number: Q4R5N4|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|