Reviewed, UniProtKB/Swiss-Prot Q4R5M2 (CATB_MACFA)

Names and origin

Protein names

Recommended name:
    Cathepsin B
    EC=3.4.22.1
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin B light chain
    2- Recommended name:
            Cathepsin B heavy chain

Gene names

Name:	CTSB
ORF Names:	QccE-13673

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis By similarity.
Catalytic activity	Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Subunit structure	Dimer of a heavy chain and a light chain cross-linked by a disulfide bond By similarity.
Subcellular location	Lysosome By similarity. Melanosome By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro regulation of catalytic activity Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 79	62	Activation peptide By similarity		PRO_0000330875
	Chain	80 – 333	254	Cathepsin B		PRO_0000330876
	Chain	80 – 126	47	Cathepsin B light chain By similarity		PRO_0000330877
	Chain	129 – 333	205	Cathepsin B heavy chain By similarity		PRO_0000330878
	Propeptide	334 – 339	6	By similarity		PRO_0000330879
Sites
	Active site	108	1	By similarity
	Active site	278	1	By similarity
	Active site	298	1	By similarity
Amino acid modifications
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Disulfide bond	93 ↔ 122		By similarity
	Disulfide bond	105 ↔ 150		By similarity
	Disulfide bond	141 ↔ 207		By similarity
	Disulfide bond	142 ↔ 146		By similarity
	Disulfide bond	179 ↔ 211		By similarity
	Disulfide bond	187 ↔ 198		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R5M2-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 57D18B0CE1CD56CF	FASTA	339	37,776
	10 20 30 40 50 60 MWWLWASLCC LLALGDARSR PSFHPLSDEL VNYVNKQNTT WQAGHNFYNV DVSYLKRLCG 70 80 90 100 110 120 TFLGGPKPPQ RVMFTEDLKL PESFDAREQW PQCPTIKEIR DQGSCGSCWA FGAVEAISDR 130 140 150 160 170 180 ICIHTNAHVS VEVSAEDLLT CCGIMCGDGC NGGYPAGAWN FWTRKGLVSG GLYDSHVGCR 190 200 210 220 230 240 PYSIPPCEHH VNGSRPPCTG EGDTPKCSKI CEPGYSPTYK QDKHYGYNSY SVSNSEKDIM 250 260 270 280 290 300 AEIYKNGPVE GAFSVYSDFL LYKSGVYQHV TGEMMGGHAI RILGWGVENG TPYWLVANSW 310 320 330 NTDWGDNGFF KILRGQDHCG IESEVVAGIP RTDQYWEKI

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169521 mRNA. Translation: BAE01603.1.
3D structure databases
SMR	Q4R5M2. Positions 18-333.
ModBase	Search...
Protein family/group databases
MEROPS	C01.060.
Phylogenomic databases
HOVERGEN	Q4R5M2.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR015643. Peptidase_C1A_cathepsin-B. IPR012599. Propeptide_C1A. [Graphical view]
PANTHER	PTHR12411:SF16. CathepsinB_like. 1 hit. PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF00112. Peptidase_C1. 1 hit. PF08127. Propeptide_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATB_MACFA

Accession

Primary (citable) accession number: Q4R5M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)