Reviewed, UniProtKB/Swiss-Prot Q4R5H0 (PIMT_MACFA)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate(D-aspartate) O-methyltransferase
      Short name=PIMT
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase
    Protein L-isoaspartyl/D-aspartyl methyltransferase
    L-isoaspartyl protein carboxyl methyltransferase

Gene names

Name:	PCMT1
ORF Names:	QnpA-11118

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 227	226	Protein-L-isoaspartate(D-aspartate) O-methyltransferase		PRO_0000253633
Sites
	Active site	60	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R5H0-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8EAAA2EC606402A8	FASTA	227	24,637
	10 20 30 40 50 60 MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AECNPYMDSP QSIGFQATIS 70 80 90 100 110 120 APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV GCTGKVIGID HIKELVDDSI 130 140 150 160 170 180 NNVRKDDPTL LSSGRVQLVV GDGRMGYAEE APYDAIHVGA AAPVVPQALI DQLKPGGRLI 190 200 210 220 LPVGPAGGNQ MLEQYDKLQD GSVKMKPLMG VIYVPLTDKE KQWSRWK

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Parietal cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169573 mRNA. Translation: BAE01655.1. Different initiation.
3D structure databases
SMR	Q4R5H0. Positions 3-226.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R5H0.
Enzyme and pathway databases
BRENDA	2.1.1.77. 3438.
Family and domain databases
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_MACFA

Accession

Primary (citable) accession number: Q4R5H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 23 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)