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Q4R5F2

- LGUL_MACFA

UniProt

Q4R5F2 - LGUL_MACFA

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Protein

Lactoylglutathione lyase

Gene

GLO1

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B (By similarity).By similarity

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc; shared with dimeric partnerBy similarity
Binding sitei34 – 341Substrate; shared with dimeric partnerBy similarity
Binding sitei38 – 381Substrate; shared with dimeric partnerBy similarity
Metal bindingi100 – 1001Zinc; shared with dimeric partnerBy similarity
Binding sitei104 – 1041Substrate; shared with dimeric partnerBy similarity
Binding sitei123 – 1231SubstrateBy similarity
Metal bindingi127 – 1271Zinc; via tele nitrogenBy similarity
Binding sitei127 – 1271SubstrateBy similarity
Active sitei173 – 1731Proton donor/acceptorBy similarity
Metal bindingi173 – 1731ZincBy similarity

GO - Molecular functioni

  1. lactoylglutathione lyase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. metabolic process Source: GOC
  2. negative regulation of apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00675.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:GLO1
ORF Names:QnpA-15219
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 184183Lactoylglutathione lyasePRO_0000168077Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Disulfide bondi19 ↔ 20By similarity
Disulfide bondi61 ↔ 139AlternateBy similarity
Modified residuei88 – 881N6-succinyllysineBy similarity
Modified residuei107 – 1071PhosphothreonineBy similarity
Modified residuei139 – 1391S-glutathionyl cysteine; alternateBy similarity
Modified residuei148 – 1481N6-acetyllysine; alternateBy similarity
Modified residuei148 – 1481N6-succinyllysine; alternateBy similarity

Post-translational modificationi

Glutathionylation at Cys-139 inhibits enzyme activity.By similarity
Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B (By similarity).By similarity
Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B (By similarity).By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Glutathionylation, Phosphoprotein

Proteomic databases

PRIDEiQ4R5F2.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ4R5F2.
SMRiQ4R5F2. Positions 2-184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1582Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glyoxalase I family.Curated

Phylogenomic databases

HOVERGENiHBG025852.
KOiK01759.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR00068. glyox_I. 1 hit.
PROSITEiPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R5F2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT
60 70 80 90 100
RVLGMTLIQK CDFPAMKFSL YFLAYEDKND IPKDKEEKIA WALSRKATLE
110 120 130 140 150
LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVHSACK RFEELGVKFV
160 170 180
KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
Length:184
Mass (Da):20,710
Last modified:January 23, 2007 - v3
Checksum:i8E8B276CB80EAB34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169591 mRNA. Translation: BAE01673.1.
RefSeqiNP_001271981.1. NM_001285052.1.
UniGeneiMfa.8289.

Genome annotation databases

GeneIDi101926610.
KEGGimcf:101926610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169591 mRNA. Translation: BAE01673.1 .
RefSeqi NP_001271981.1. NM_001285052.1.
UniGenei Mfa.8289.

3D structure databases

ProteinModelPortali Q4R5F2.
SMRi Q4R5F2. Positions 2-184.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q4R5F2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101926610.
KEGGi mcf:101926610.

Organism-specific databases

CTDi 2739.

Phylogenomic databases

HOVERGENi HBG025852.
KOi K01759.

Enzyme and pathway databases

UniPathwayi UPA00619 ; UER00675 .

Family and domain databases

Gene3Di 3.10.180.10. 1 hit.
InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view ]
Pfami PF00903. Glyoxalase. 1 hit.
[Graphical view ]
SUPFAMi SSF54593. SSF54593. 1 hit.
TIGRFAMsi TIGR00068. glyox_I. 1 hit.
PROSITEi PS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Parietal cortex.

Entry informationi

Entry nameiLGUL_MACFA
AccessioniPrimary (citable) accession number: Q4R5F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3