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Q4R5F2

- LGUL_MACFA

UniProt

Q4R5F2 - LGUL_MACFA

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Protein

Lactoylglutathione lyase

Gene
GLO1, QnpA-15219
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B By similarity.

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc; shared with dimeric partner By similarity
Binding sitei34 – 341Substrate; shared with dimeric partner By similarity
Binding sitei38 – 381Substrate; shared with dimeric partner By similarity
Metal bindingi100 – 1001Zinc; shared with dimeric partner By similarity
Binding sitei104 – 1041Substrate; shared with dimeric partner By similarity
Binding sitei123 – 1231Substrate By similarity
Metal bindingi127 – 1271Zinc; via tele nitrogen By similarity
Binding sitei127 – 1271Substrate By similarity
Active sitei173 – 1731Proton donor/acceptor By similarity
Metal bindingi173 – 1731Zinc By similarity

GO - Molecular functioni

  1. lactoylglutathione lyase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. metabolic process Source: GOC
  2. negative regulation of apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00675.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:GLO1
ORF Names:QnpA-15219
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 184183Lactoylglutathione lyasePRO_0000168077Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Disulfide bondi19 ↔ 20 By similarity
Disulfide bondi61 ↔ 139Alternate By similarity
Modified residuei88 – 881N6-succinyllysine By similarity
Modified residuei107 – 1071Phosphothreonine By similarity
Modified residuei139 – 1391S-glutathionyl cysteine; alternate By similarity
Modified residuei148 – 1481N6-acetyllysine; alternate By similarity
Modified residuei148 – 1481N6-succinyllysine; alternate By similarity

Post-translational modificationi

Glutathionylation at Cys-139 inhibits enzyme activity By similarity.
Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B By similarity.
Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B By similarity.

Keywords - PTMi

Acetylation, Disulfide bond, Glutathionylation, Phosphoprotein

Proteomic databases

PRIDEiQ4R5F2.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ4R5F2.
SMRiQ4R5F2. Positions 2-184.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1582Substrate binding By similarity

Sequence similaritiesi

Belongs to the glyoxalase I family.

Phylogenomic databases

HOVERGENiHBG025852.
KOiK01759.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR00068. glyox_I. 1 hit.
PROSITEiPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R5F2-1 [UniParc]FASTAAdd to Basket

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MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT    50
RVLGMTLIQK CDFPAMKFSL YFLAYEDKND IPKDKEEKIA WALSRKATLE 100
LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVHSACK RFEELGVKFV 150
KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM 184
Length:184
Mass (Da):20,710
Last modified:January 23, 2007 - v3
Checksum:i8E8B276CB80EAB34
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169591 mRNA. Translation: BAE01673.1.
RefSeqiNP_001271981.1. NM_001285052.1.
UniGeneiMfa.8289.

Genome annotation databases

GeneIDi101926610.
KEGGimcf:101926610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169591 mRNA. Translation: BAE01673.1 .
RefSeqi NP_001271981.1. NM_001285052.1.
UniGenei Mfa.8289.

3D structure databases

ProteinModelPortali Q4R5F2.
SMRi Q4R5F2. Positions 2-184.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q4R5F2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101926610.
KEGGi mcf:101926610.

Organism-specific databases

CTDi 2739.

Phylogenomic databases

HOVERGENi HBG025852.
KOi K01759.

Enzyme and pathway databases

UniPathwayi UPA00619 ; UER00675 .

Family and domain databases

Gene3Di 3.10.180.10. 1 hit.
InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view ]
Pfami PF00903. Glyoxalase. 1 hit.
[Graphical view ]
SUPFAMi SSF54593. SSF54593. 1 hit.
TIGRFAMsi TIGR00068. glyox_I. 1 hit.
PROSITEi PS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Parietal cortex.

Entry informationi

Entry nameiLGUL_MACFA
AccessioniPrimary (citable) accession number: Q4R5F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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