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Q4R5B8

- EIF3F_MACFA

UniProt

Q4R5B8 - EIF3F_MACFA

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Protein

Eukaryotic translation initiation factor 3 subunit F

Gene
EIF3F, EIF3S5, QtrA-12369
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation By similarity.UniRule annotation
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling By similarity.UniRule annotation

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. translation initiation factor activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
  2. regulation of translational initiation Source: UniProtKB-HAMAP
  3. translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Initiation factor, Protease, Thiol protease

Keywords - Biological processi

Protein biosynthesis, Ubl conjugation pathway

Protein family/group databases

MEROPSiM67.974.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit F
Short name:
eIF3f
Alternative name(s):
Deubiquitinating enzyme eIF3f (EC:3.4.19.12)
Eukaryotic translation initiation factor 3 subunit 5
eIF-3-epsilon
eIF3 p47
Gene namesi
Name:EIF3F
Synonyms:EIF3S5
ORF Names:QtrA-12369
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
  2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  3. eukaryotic translation initiation factor 3 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 361360Eukaryotic translation initiation factor 3 subunit FUniRule annotationPRO_0000297558Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei50 – 501Phosphoserine; by CDK11; in vitro By similarity
Modified residuei242 – 2421N6-acetyllysine By similarity
Modified residuei262 – 2621Phosphoserine By similarity

Post-translational modificationi

Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11 By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Phosphoprotein

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ4R5B8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 200110MPNAdd
BLAST

Domaini

The MPN domain mediates deubiquitinating activity By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the eIF-3 subunit F family.

Phylogenomic databases

HOVERGENiHBG107843.
KOiK03249.

Family and domain databases

HAMAPiMF_03005. eIF3f.
InterProiIPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R5B8-1 [UniParc]FASTAAdd to Basket

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MATPVVSASG PPATPAPAPV AAPASASASV PAPTPAPAAA AVPAAAPAAS    50
SDPAAAAATT AAPGQTPASA QAPAQTPAPA LPGPALPGPF PGGRVVRLHP 100
VILASIVDSY ERRNEGAARV IGTLLGTVDK HSVEVTNCFS VPHNESEDEV 150
AVDMEFAKNM YELHKKVSPN ELILGWYATG HDITEHSVLI HEYYSREAPN 200
PIHLTVDTSL QNGRMSIKAY VSTLMGVPGR TMGVMFTPLT VKYAYYDTER 250
IGVDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG 300
KVSADNTVGR FLMSLVNQVP KIVPDDFETM LNSNINDLLM VTYLANLTQS 350
QIALNEKLVN L 361
Length:361
Mass (Da):37,814
Last modified:July 19, 2005 - v1
Checksum:i01DCFBE8C7834AE9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169626 mRNA. Translation: BAE01707.1.
RefSeqiNP_001271708.1. NM_001284779.1.
UniGeneiMfa.8337.

Genome annotation databases

GeneIDi102137776.
KEGGimcf:102137776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169626 mRNA. Translation: BAE01707.1 .
RefSeqi NP_001271708.1. NM_001284779.1.
UniGenei Mfa.8337.

3D structure databases

ProteinModelPortali Q4R5B8.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M67.974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102137776.
KEGGi mcf:102137776.

Organism-specific databases

CTDi 8665.

Phylogenomic databases

HOVERGENi HBG107843.
KOi K03249.

Family and domain databases

HAMAPi MF_03005. eIF3f.
InterProi IPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view ]
Pfami PF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view ]
SMARTi SM00232. JAB_MPN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Temporal cortex.

Entry informationi

Entry nameiEIF3F_MACFA
AccessioniPrimary (citable) accession number: Q4R5B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 19, 2005
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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