Q4R5B8 (EIF3F_MACFA) Reviewed, UniProtKB/Swiss-Prot
Last modified November 13, 2013. Version 44. History...
Names and origin
|Protein names||Recommended name:|
Eukaryotic translation initiation factor 3 subunit F
Deubiquitinating enzyme eIF3f
Eukaryotic translation initiation factor 3 subunit 5
|Organism||Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)|
|Taxonomic identifier||9541 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca|
|Sequence length||361 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation By similarity. HAMAP-Rule MF_03005
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling By similarity. HAMAP-Rule MF_03005
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). HAMAP-Rule MF_03005
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 By similarity.
The MPN domain mediates deubiquitinating activity By similarity. HAMAP-Rule MF_03005
Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11 By similarity. HAMAP-Rule MF_03005
Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed By similarity|
|Chain||2 – 361||360||Eukaryotic translation initiation factor 3 subunit F HAMAP-Rule MF_03005||PRO_0000297558|
|Domain||91 – 200||110||MPN|
Amino acid modifications
|Modified residue||2||1||N-acetylalanine By similarity|
|Modified residue||50||1||Phosphoserine; by CDK11; in vitro By similarity|
|Modified residue||242||1||N6-acetyllysine By similarity|
|Modified residue||262||1||Phosphoserine By similarity|
|||"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."|
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Temporal cortex.
|AB169626 mRNA. Translation: BAE01707.1.|
3D structure databases
Protein family/group databases
Protocols and materials databases
Family and domain databases
|HAMAP||MF_03005. eIF3f. |
|InterPro||IPR027531. eIF3f. |
|Pfam||PF01398. JAB. 1 hit. |
PF13012. MitMem_reg. 1 hit.
|SMART||SM00232. JAB_MPN. 1 hit. |
|Accession||Primary (citable) accession number: Q4R5B8|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|