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Q4R5B8 (EIF3F_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 3 subunit F

Short name=eIF3f
Alternative name(s):
Deubiquitinating enzyme eIF3f
EC=3.4.19.12
Eukaryotic translation initiation factor 3 subunit 5
eIF-3-epsilon
eIF3 p47
Gene names
Name:EIF3F
Synonyms:EIF3S5
ORF Names:QtrA-12369
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation By similarity. HAMAP-Rule MF_03005

Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling By similarity. HAMAP-Rule MF_03005

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). HAMAP-Rule MF_03005

Subunit structure

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_03005.

Domain

The MPN domain mediates deubiquitinating activity By similarity. HAMAP-Rule MF_03005

Post-translational modification

Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11 By similarity. HAMAP-Rule MF_03005

Sequence similarities

Belongs to the eIF-3 subunit F family.

Contains 1 MPN (JAB/Mov34) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 361360Eukaryotic translation initiation factor 3 subunit F HAMAP-Rule MF_03005
PRO_0000297558

Regions

Domain91 – 200110MPN

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue501Phosphoserine; by CDK11; in vitro By similarity
Modified residue2421N6-acetyllysine By similarity
Modified residue2621Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4R5B8 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 01DCFBE8C7834AE9

FASTA36137,814
        10         20         30         40         50         60 
MATPVVSASG PPATPAPAPV AAPASASASV PAPTPAPAAA AVPAAAPAAS SDPAAAAATT 

        70         80         90        100        110        120 
AAPGQTPASA QAPAQTPAPA LPGPALPGPF PGGRVVRLHP VILASIVDSY ERRNEGAARV 

       130        140        150        160        170        180 
IGTLLGTVDK HSVEVTNCFS VPHNESEDEV AVDMEFAKNM YELHKKVSPN ELILGWYATG 

       190        200        210        220        230        240 
HDITEHSVLI HEYYSREAPN PIHLTVDTSL QNGRMSIKAY VSTLMGVPGR TMGVMFTPLT 

       250        260        270        280        290        300 
VKYAYYDTER IGVDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG 

       310        320        330        340        350        360 
KVSADNTVGR FLMSLVNQVP KIVPDDFETM LNSNINDLLM VTYLANLTQS QIALNEKLVN 


L 

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References

[1]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Temporal cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB169626 mRNA. Translation: BAE01707.1.
RefSeqNP_001271708.1. NM_001284779.1.
UniGeneMfa.8337.

3D structure databases

ProteinModelPortalQ4R5B8.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM67.974.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102137776.
KEGGmcf:102137776.

Organism-specific databases

CTD8665.

Phylogenomic databases

HOVERGENHBG107843.
KOK03249.

Family and domain databases

HAMAPMF_03005. eIF3f.
InterProIPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEIF3F_MACFA
AccessionPrimary (citable) accession number: Q4R5B8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries