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Q4R5B8

- EIF3F_MACFA

UniProt

Q4R5B8 - EIF3F_MACFA

Protein

Eukaryotic translation initiation factor 3 subunit F

Gene

EIF3F

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.UniRule annotation
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. translation initiation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
    2. regulation of translational initiation Source: UniProtKB-HAMAP
    3. translational initiation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Initiation factor, Protease, Thiol protease

    Keywords - Biological processi

    Protein biosynthesis, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiM67.974.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 3 subunit FUniRule annotation
    Short name:
    eIF3fUniRule annotation
    Alternative name(s):
    Deubiquitinating enzyme eIF3f (EC:3.4.19.12)
    Eukaryotic translation initiation factor 3 subunit 5UniRule annotation
    eIF-3-epsilonUniRule annotation
    eIF3 p47UniRule annotation
    Gene namesi
    Name:EIF3FUniRule annotation
    Synonyms:EIF3S5UniRule annotation
    ORF Names:QtrA-12369
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
    2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
    3. eukaryotic translation initiation factor 3 complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 361360Eukaryotic translation initiation factor 3 subunit FPRO_0000297558Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineUniRule annotation
    Modified residuei50 – 501Phosphoserine; by CDK11; in vitroUniRule annotation
    Modified residuei242 – 2421N6-acetyllysineBy similarity
    Modified residuei262 – 2621PhosphoserineUniRule annotation

    Post-translational modificationi

    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Interactioni

    Subunit structurei

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 By similarity.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ4R5B8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 200110MPNUniRule annotationAdd
    BLAST

    Domaini

    The MPN domain mediates deubiquitinating activity.By similarity

    Sequence similaritiesi

    Belongs to the eIF-3 subunit F family.UniRule annotation
    Contains 1 MPN (JAB/Mov34) domain.UniRule annotation

    Phylogenomic databases

    HOVERGENiHBG107843.
    KOiK03249.

    Family and domain databases

    HAMAPiMF_03005. eIF3f.
    InterProiIPR027531. eIF3f.
    IPR000555. JAMM/MPN+_dom.
    IPR024969. Rpn11/EIF3F_C.
    [Graphical view]
    PfamiPF01398. JAB. 1 hit.
    PF13012. MitMem_reg. 1 hit.
    [Graphical view]
    SMARTiSM00232. JAB_MPN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4R5B8-1 [UniParc]FASTAAdd to Basket

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    MATPVVSASG PPATPAPAPV AAPASASASV PAPTPAPAAA AVPAAAPAAS    50
    SDPAAAAATT AAPGQTPASA QAPAQTPAPA LPGPALPGPF PGGRVVRLHP 100
    VILASIVDSY ERRNEGAARV IGTLLGTVDK HSVEVTNCFS VPHNESEDEV 150
    AVDMEFAKNM YELHKKVSPN ELILGWYATG HDITEHSVLI HEYYSREAPN 200
    PIHLTVDTSL QNGRMSIKAY VSTLMGVPGR TMGVMFTPLT VKYAYYDTER 250
    IGVDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG 300
    KVSADNTVGR FLMSLVNQVP KIVPDDFETM LNSNINDLLM VTYLANLTQS 350
    QIALNEKLVN L 361
    Length:361
    Mass (Da):37,814
    Last modified:July 19, 2005 - v1
    Checksum:i01DCFBE8C7834AE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169626 mRNA. Translation: BAE01707.1.
    RefSeqiNP_001271708.1. NM_001284779.1.
    UniGeneiMfa.8337.

    Genome annotation databases

    GeneIDi102137776.
    KEGGimcf:102137776.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169626 mRNA. Translation: BAE01707.1 .
    RefSeqi NP_001271708.1. NM_001284779.1.
    UniGenei Mfa.8337.

    3D structure databases

    ProteinModelPortali Q4R5B8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M67.974.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 102137776.
    KEGGi mcf:102137776.

    Organism-specific databases

    CTDi 8665.

    Phylogenomic databases

    HOVERGENi HBG107843.
    KOi K03249.

    Family and domain databases

    HAMAPi MF_03005. eIF3f.
    InterProi IPR027531. eIF3f.
    IPR000555. JAMM/MPN+_dom.
    IPR024969. Rpn11/EIF3F_C.
    [Graphical view ]
    Pfami PF01398. JAB. 1 hit.
    PF13012. MitMem_reg. 1 hit.
    [Graphical view ]
    SMARTi SM00232. JAB_MPN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Temporal cortex.

    Entry informationi

    Entry nameiEIF3F_MACFA
    AccessioniPrimary (citable) accession number: Q4R5B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 21, 2007
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3