Reviewed, UniProtKB/Swiss-Prot Q4R583 (PLD3_MACFA)

Names and origin

Protein names

Recommended name:
    Phospholipase D3
      Short name=PLD 3
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 3
    Phosphatidylcholine-hydrolyzing phospholipase D3

Gene names

Name:	PLD3
ORF Names:	QccE-15167

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Endoplasmic reticulum Membrane
Domain	Repeat Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Phospholipase D3		PRO_0000280327
Regions
	Topological domain	1 – 38	38	Cytoplasmic Potential
	Transmembrane	39 – 59	21	Signal-anchor for type II membrane protein Potential
	Topological domain	60 – 490	431	Lumenal Potential
	Domain	196 – 223	28	PLD phosphodiesterase 1
	Domain	411 – 437	27	PLD phosphodiesterase 2
Sites
	Active site	201	1	Potential
	Active site	203	1	Potential
	Active site	208	1	Potential
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4R583-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: ED2891E1920AB9DF	FASTA	490	54,852
	10 20 30 40 50 60 MKPKLMYQEL KVPAEEPANE LPMNEIEAWK AAEKKARWVL LVLILAVVGF GALMTQLFLW 70 80 90 100 110 120 EYGDLHLFGP NQRPAPCYDP CEAVLVESIP EGLDFPNAST GNPSTSQAWL GLLAGAHSSL 130 140 150 160 170 180 DIASFYWTLT NNDTHTQEPS AQQGEEVLRQ LQTLAPKGVN VRIAVSKPNG PQPQTDLQAL 190 200 210 220 230 240 LQSGAQVRMV DMQKLTHGVL HTKFWVVDQT HFYLGSANMD WRSLTQVKEL GVVMYNCSCL 250 260 270 280 290 300 ARDLTKIFEA YWFLGQAGSS IPSTWPRFYD TRYNQETPME ICLNGTPALA YLASAPPPLC 310 320 330 340 350 360 PSGRTPDLKA LLNVVDNARS FIYIAVMNYL PTLEFSHPHR FWPAIDDGLR RAAYERGVKV 370 380 390 400 410 420 RLLVSCWRHS ESSMRAFLLS LAALRDNHTH SDIQVKLFVV PADEAQARIP YARVNHNKYM 430 440 450 460 470 480 VTERATYIGT SNWSGNYFTE TAGTSLLVMQ NGRGSLRSQL EAIFLRDWDS PYSHDLDASA 490 DSVGNACRLL

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169661 mRNA. Translation: BAE01742.1.
3D structure databases
SMR	Q4R583. Positions 105-257.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R583.
Enzyme and pathway databases
BRENDA	3.1.4.4. 3438.
Family and domain databases
InterPro	IPR013582. PLD_envelope. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF08371. PLD_envelope. 1 hit. PF00614. PLDc. 1 hit. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLD3_MACFA

Accession

Primary (citable) accession number: Q4R583

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 19, 2005
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)