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Protein

14-3-3 protein beta/alpha

Gene

YWHAB

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13.By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein beta/alpha
Cleaved into the following chain:
Gene namesi
Name:YWHAB
ORF Names:QccE-16215
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

  • Cytoplasm By similarity
  • Melanosome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24624614-3-3 protein beta/alphaPRO_0000367901Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 24624514-3-3 protein beta/alpha, N-terminally processedPRO_0000042983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processedBy similarity
Modified residuei2 – 21PhosphothreonineBy similarity
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei70 – 701N6-acetyllysineBy similarity
Modified residuei84 – 841Nitrated tyrosineBy similarity
Modified residuei106 – 1061Nitrated tyrosineBy similarity
Modified residuei117 – 1171N6-acetyllysineBy similarity
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei232 – 2321PhosphoserineBy similarity
Isoform Short (identifier: Q4R572-2)
Modified residuei1 – 11N-acetylmethionine By similarityBy similarity

Post-translational modificationi

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.By similarity
Isoform Short contains a N-acetylmethionine at position 1.By similarity

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PRIDEiQ4R572.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with SAMSN1 and PRKCE (By similarity). Interacts with AKAP13. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2. Interacts with YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Interacts with MYO1C. Interacts with SIRT2 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei58 – 581Interaction with phosphoserine on interacting proteinBy similarity
Sitei129 – 1291Interaction with phosphoserine on interacting proteinBy similarity

Protein-protein interaction databases

IntActiQ4R572. 1 interaction.
MINTiMINT-7997439.

Structurei

3D structure databases

ProteinModelPortaliQ4R572.
SMRiQ4R572. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

HOVERGENiHBG050423.
KOiK16197.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: Q4R572-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY
60 70 80 90 100
KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL
110 120 130 140 150
ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA
160 170 180 190 200
YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE
210 220 230 240
AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Length:246
Mass (Da):28,082
Last modified:January 23, 2007 - v3
Checksum:i6BE1A9BF97468017
GO
Isoform Short (identifier: Q4R572-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: No experimental confirmation available. Inferred by similarity.By similarity
Show »
Length:244
Mass (Da):27,850
Checksum:iCE0A59BF8C33FE2F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 22Missing in isoform Short. CuratedVSP_018633

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169672 mRNA. Translation: BAE01753.1.
RefSeqiXP_005569168.1. XM_005569111.2. [Q4R572-1]
XP_005569169.1. XM_005569112.1. [Q4R572-1]
XP_005569170.1. XM_005569113.1. [Q4R572-1]
UniGeneiMfa.2560.

Genome annotation databases

GeneIDi101926442.
KEGGimcf:101926442.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB169672 mRNA. Translation: BAE01753.1.
RefSeqiXP_005569168.1. XM_005569111.2. [Q4R572-1]
XP_005569169.1. XM_005569112.1. [Q4R572-1]
XP_005569170.1. XM_005569113.1. [Q4R572-1]
UniGeneiMfa.2560.

3D structure databases

ProteinModelPortaliQ4R572.
SMRiQ4R572. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4R572. 1 interaction.
MINTiMINT-7997439.

Proteomic databases

PRIDEiQ4R572.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101926442.
KEGGimcf:101926442.

Organism-specific databases

CTDi7529.

Phylogenomic databases

HOVERGENiHBG050423.
KOiK16197.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry namei1433B_MACFA
AccessioniPrimary (citable) accession number: Q4R572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.