Reviewed, UniProtKB/Swiss-Prot Q4R568 (MDHM_MACFA)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, mitochondrial
    EC=1.1.1.37

Gene names

Name:	MDH2
ORF Names:	QccE-17106

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion By similarity
	Chain	25 – 338	314	Malate dehydrogenase, mitochondrial		PRO_0000285846
Regions
	Nucleotide binding	31 – 37	7	NAD By similarity
	Nucleotide binding	140 – 142	3	NAD By similarity
Sites
	Active site	200	1	Proton acceptor By similarity
	Binding site	57	1	NAD By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	117	1	NAD By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	251	1	NAD By similarity
Amino acid modifications
	Modified residue	56	1	Phosphotyrosine By similarity
	Modified residue	157	1	N6-acetyllysine By similarity
	Modified residue	165	1	N6-acetyllysine By similarity
	Modified residue	185	1	N6-acetyllysine By similarity
	Modified residue	239	1	N6-acetyllysine By similarity
	Modified residue	301	1	N6-acetyllysine By similarity
	Modified residue	314	1	N6-acetyllysine By similarity
	Modified residue	329	1	N6-acetyllysine By similarity
	Modified residue	335	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R568-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: E2C104CDFD3F468F	FASTA	338	35,565
	10 20 30 40 50 60 MLSALARPAS AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 70 80 90 100 110 120 TPGVAADLSH IETKAVVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 130 140 150 160 170 180 VATLAAACAQ HRPEAMICII ANPVNSTIPI TAEVFKKHGV YNPSKIFGVT TLDIVRANTF 190 200 210 220 230 240 VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLTALTGRI QEAGTEVVKA 250 260 270 280 290 300 KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETECTYFST PLLLGKKGIE 310 320 330 KNLGIGQIPS FEEKMISDAI PELKASIKKG EDFVKTLK

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169676 mRNA. Translation: BAE01757.1.
3D structure databases
SMR	Q4R568. Positions 24-337.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R568.
Enzyme and pathway databases
BRENDA	1.1.1.37. 3438.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk/g-bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHM_MACFA

Accession

Primary (citable) accession number: Q4R568

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 19, 2005
Last modified:	October 13, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)