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Q4R568

- MDHM_MACFA

UniProt

Q4R568 - MDHM_MACFA

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Protein

Malate dehydrogenase, mitochondrial

Gene
MDH2, QccE-17106
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Enzyme regulationi

Enzyme activity is enhanced by acetylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571NAD By similarity
Binding sitei104 – 1041Substrate By similarity
Binding sitei110 – 1101Substrate By similarity
Binding sitei117 – 1171NAD By similarity
Binding sitei142 – 1421Substrate By similarity
Binding sitei176 – 1761Substrate By similarity
Active sitei200 – 2001Proton acceptor By similarity
Binding sitei251 – 2511NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 377NAD By similarity
Nucleotide bindingi140 – 1423NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. internal protein amino acid acetylation Source: UniProtKB
  3. malate metabolic process Source: InterPro
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:MDH2
ORF Names:QccE-17106
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion By similarityAdd
BLAST
Chaini25 – 338314Malate dehydrogenase, mitochondrialPRO_0000285846Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331O-linked (GlcNAc) By similarity
Modified residuei78 – 781N6-acetyllysine; alternate By similarity
Modified residuei78 – 781N6-succinyllysine; alternate By similarity
Modified residuei91 – 911N6-acetyllysine; alternate By similarity
Modified residuei91 – 911N6-succinyllysine; alternate By similarity
Modified residuei165 – 1651N6-acetyllysine By similarity
Modified residuei185 – 1851N6-acetyllysine; alternate By similarity
Modified residuei185 – 1851N6-succinyllysine; alternate By similarity
Modified residuei203 – 2031N6-succinyllysine By similarity
Modified residuei215 – 2151N6-acetyllysine; alternate By similarity
Modified residuei215 – 2151N6-succinyllysine; alternate By similarity
Modified residuei239 – 2391N6-acetyllysine; alternate By similarity
Modified residuei239 – 2391N6-malonyllysine; alternate By similarity
Modified residuei239 – 2391N6-succinyllysine; alternate By similarity
Modified residuei269 – 2691N6-succinyllysine By similarity
Modified residuei296 – 2961N6-acetyllysine; alternate By similarity
Modified residuei296 – 2961N6-succinyllysine; alternate By similarity
Modified residuei301 – 3011N6-acetyllysine; alternate By similarity
Modified residuei301 – 3011N6-succinyllysine; alternate By similarity
Modified residuei314 – 3141N6-acetyllysine; alternate By similarity
Modified residuei314 – 3141N6-succinyllysine; alternate By similarity
Modified residuei324 – 3241N6-acetyllysine; alternate By similarity
Modified residuei324 – 3241N6-succinyllysine; alternate By similarity
Modified residuei328 – 3281N6-acetyllysine; alternate By similarity
Modified residuei328 – 3281N6-succinyllysine; alternate By similarity
Modified residuei329 – 3291N6-acetyllysine; alternate By similarity
Modified residuei329 – 3291N6-malonyllysine; alternate By similarity
Modified residuei335 – 3351N6-acetyllysine; alternate By similarity
Modified residuei335 – 3351N6-succinyllysine; alternate By similarity

Post-translational modificationi

Acetylation is enhanced by up to 67% after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60% By similarity.

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PRIDEiQ4R568.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ4R568.
SMRiQ4R568. Positions 24-337.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG001662.
KOiK00026.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R568-1 [UniParc]FASTAAdd to Basket

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MLSALARPAS AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV    50
SRLTLYDIAH TPGVAADLSH IETKAVVKGY LGPEQLPDCL KGCDVVVIPA 100
GVPRKPGMTR DDLFNTNATI VATLAAACAQ HRPEAMICII ANPVNSTIPI 150
TAEVFKKHGV YNPSKIFGVT TLDIVRANTF VAELKGLDPA RVNVPVIGGH 200
AGKTIIPLIS QCTPKVDFPQ DQLTALTGRI QEAGTEVVKA KAGAGSATLS 250
MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETECTYFST PLLLGKKGIE 300
KNLGIGQIPS FEEKMISDAI PELKASIKKG EDFVKTLK 338
Length:338
Mass (Da):35,565
Last modified:July 19, 2005 - v1
Checksum:iE2C104CDFD3F468F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169676 mRNA. Translation: BAE01757.1.
RefSeqiNP_001270089.1. NM_001283160.1.
UniGeneiMfa.8570.

Genome annotation databases

GeneIDi101867276.
KEGGimcf:101867276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB169676 mRNA. Translation: BAE01757.1 .
RefSeqi NP_001270089.1. NM_001283160.1.
UniGenei Mfa.8570.

3D structure databases

ProteinModelPortali Q4R568.
SMRi Q4R568. Positions 24-337.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q4R568.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101867276.
KEGGi mcf:101867276.

Organism-specific databases

CTDi 4191.

Phylogenomic databases

HOVERGENi HBG001662.
KOi K00026.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry nameiMDHM_MACFA
AccessioniPrimary (citable) accession number: Q4R568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 19, 2005
Last modified: September 3, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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