Reviewed, UniProtKB/Swiss-Prot Q4R550 (SYCC_MACFA)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	CARS
ORF Names:	QccE-19031

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Cysteinyl-tRNA synthetase, cytoplasmic		PRO_0000250744
Regions
	Motif	57 – 67	11	"HIGH" region
	Motif	406 – 410	5	"KMSKS" region
Sites
	Metal binding	55	1	Zinc By similarity
	Metal binding	348	1	Zinc By similarity
	Metal binding	373	1	Zinc By similarity
	Metal binding	377	1	Zinc By similarity
	Binding site	409	1	ATP By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	260	1	Phosphotyrosine By similarity
	Modified residue	307	1	Phosphoserine By similarity
	Modified residue	746	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R550-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 8809DBB18338B7CF	FASTA	748	85,452
	10 20 30 40 50 60 MAGSSGQQGK GRRVQPQWSP PAGTQPCRLH LYNSLTRNKE VFIPQDGKKV TWYCCGPTVY 70 80 90 100 110 120 DASHMGHARS YISFDILRRV LKDYFKFDVF YCMNITDIDD KIIKRARQNH LFEQYLEKRP 130 140 150 160 170 180 EAAQLLEDVQ AALKPFSVKL NETTDPDKKQ MLERIQHEVQ LATEPLEKAV QSRLTGEEVN 190 200 210 220 230 240 SCVEVLLEEA KDLLSDWLDS TLGSDVTDNS IFSKLPKFWE GEFHRDMEAL NVLPPDVLTR 250 260 270 280 290 300 VSEYVPEIVN FVQKIVDNGY GYVSDGSVYF DTAKFASSEK HSYGKLVPEA VGDQKALQEG 310 320 330 340 350 360 EGDLSISADR LSEKRSPNDF ALWKASKPGE PSWPCPWGKG RPGWHIECSA MAGTLLGASM 370 380 390 400 410 420 DIHGGGFDLR FPHHDNELAQ SEAYFENDCW VRYFLHTGHL TITGCKMSKS LKNFITIKDA 430 440 450 460 470 480 LKKHSARQLR LAFLMHSWKD TLDYSSNTME SALQYEKFLN EFFLNVKDIL RAPVDITGQF 490 500 510 520 530 540 EKWGEEEAEL NKNFYDKKTA IHEALCDNVD TRTVMEEMRA LVSQCNLYMA ARKAMRKRPN 550 560 570 580 590 600 RALLENIALY LTHMLKIFGA IEEESSLGFP VGGSGTSLNL ESTVMPYLQV LSEFREGVRK 610 620 630 640 650 660 IAREQKVPEV LQLSDALRDD ILPELGVRFE DPEGLPTVVK LVDRNTLLKE REEKRQVEEE 670 680 690 700 710 720 KRKKKEEAAR RKQEQEAAKL AKMKIPPSEM FLSETDKYSK FDENGLPTHD AEGKELSKGQ 730 740 AKKVKKLFEA QEKLYKEYLQ MPQNGSFQ

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169694 mRNA. Translation: BAE01775.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
SMR	Q4R550. Positions 29-564.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R550.
Enzyme and pathway databases
BRENDA	6.1.1.16. 3438.
Family and domain databases
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_MACFA

Accession

Primary (citable) accession number: Q4R550

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	July 19, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)