Reviewed, UniProtKB/Swiss-Prot Q4R549 (MIF_MACFA)

Names and origin

Protein names

Recommended name:
    Macrophage migration inhibitory factor
      Short name=MIF
    EC=5.3.2.1
Alternative name(s):
    Phenylpyruvate tautomerase

Gene names

Name:	MIF
ORF Names:	QccE-19130

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The expression of MIF at sites of inflammation suggest a role for the mediator in regulating the function of macrophage in host defense. Also acts as a phenylpyruvate tautomerase By similarity.
Catalytic activity	Keto-phenylpyruvate = enol-phenylpyruvate.
Subunit structure	Homotrimer. Interacts with COPS5 and BNIPL By similarity.
Sequence similarities	Belongs to the MIF family.

Ontologies

Keywords
Biological process	Inflammatory response
Molecular function	Cytokine Isomerase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW phenylpyruvate tautomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 115	114	Macrophage migration inhibitory factor		PRO_0000158063
Sites
	Active site	2	1	Proton acceptor; via imino nitrogen By similarity
Amino acid modifications
	Modified residue	37	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R549-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 56D51107C05286B2	FASTA	115	12,476
	10 20 30 40 50 60 MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC 70 80 90 100 110 SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169695 mRNA. Translation: BAE01776.1.
3D structure databases
SMR	Q4R549. Positions 1-114, 2-115.
ModBase	Search...
Proteomic databases
PRIDE	Q4R549.
Phylogenomic databases
HOVERGEN	Q4R549.
Enzyme and pathway databases
BRENDA	5.3.2.1. 3438.
Family and domain databases
InterPro	IPR001398. Macrophage_inhib_fac. IPR019829. Macrophage_inhib_fac_CS. IPR014347. Tautomerase. [Graphical view]
Gene3D	G3DSA:3.30.429.10. Tautomerase. 1 hit.
PANTHER	PTHR11954. MIF. 1 hit.
Pfam	PF01187. MIF. 1 hit. [Graphical view]
ProDom	PD004816. MIF. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01158. MIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIF_MACFA

Accession

Primary (citable) accession number: Q4R549

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 27 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)