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Q4R549

- MIF_MACFA

UniProt

Q4R549 - MIF_MACFA

Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
  1. Functioni

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.By similarity

    Catalytic activityi

    Keto-phenylpyruvate = enol-phenylpyruvate.
    L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
    Binding sitei33 – 331SubstrateBy similarity
    Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
    Binding sitei98 – 981SubstrateBy similarity

    GO - Molecular functioni

    1. dopachrome isomerase activity Source: UniProtKB
    2. phenylpyruvate tautomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. inflammatory response Source: UniProtKB-KW
    2. innate immune response Source: UniProtKB-KW
    3. metabolic process Source: GOC

    Keywords - Molecular functioni

    Cytokine, Isomerase

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Macrophage migration inhibitory factor (EC:5.3.2.1)
    Short name:
    MIF
    Alternative name(s):
    L-dopachrome isomerase
    L-dopachrome tautomerase (EC:5.3.3.12)
    Phenylpyruvate tautomerase
    Gene namesi
    Name:MIF
    ORF Names:QccE-19130
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Secreted By similarity. Cytoplasm By similarity
    Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781N6-acetyllysine; alternateBy similarity
    Modified residuei78 – 781N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ4R549.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ4R549.
    SMRiQ4R549. Positions 2-115.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MIF family.Curated

    Phylogenomic databases

    HOVERGENiHBG003240.
    KOiK07253.

    Family and domain databases

    InterProiIPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view]
    PANTHERiPTHR11954. PTHR11954. 1 hit.
    PfamiPF01187. MIF. 1 hit.
    [Graphical view]
    ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55331. SSF55331. 1 hit.
    PROSITEiPS01158. MIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4R549-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF    50
    GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY 100
    DMNAANVGWN NSTFA 115
    Length:115
    Mass (Da):12,476
    Last modified:January 23, 2007 - v3
    Checksum:i56D51107C05286B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169695 mRNA. Translation: BAE01776.1.
    RefSeqiNP_001270626.1. NM_001283697.1.
    UniGeneiMfa.887.

    Genome annotation databases

    GeneIDi101867092.
    KEGGimcf:101867092.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169695 mRNA. Translation: BAE01776.1 .
    RefSeqi NP_001270626.1. NM_001283697.1.
    UniGenei Mfa.887.

    3D structure databases

    ProteinModelPortali Q4R549.
    SMRi Q4R549. Positions 2-115.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q4R549.

    Proteomic databases

    PRIDEi Q4R549.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101867092.
    KEGGi mcf:101867092.

    Organism-specific databases

    CTDi 4282.

    Phylogenomic databases

    HOVERGENi HBG003240.
    KOi K07253.

    Family and domain databases

    InterProi IPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view ]
    PANTHERi PTHR11954. PTHR11954. 1 hit.
    Pfami PF01187. MIF. 1 hit.
    [Graphical view ]
    ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55331. SSF55331. 1 hit.
    PROSITEi PS01158. MIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiMIF_MACFA
    AccessioniPrimary (citable) accession number: Q4R549
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3