Reviewed, UniProtKB/Swiss-Prot Q4R502 (IDHP_MACFA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP
    ICD-M

Gene names

Name:	IDH2
ORF Names:	QnpA-13564

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex By similarity.
Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 452	413	Isocitrate dehydrogenase [NADP], mitochondrial		PRO_0000236186
Regions
	Nucleotide binding	115 – 117	3	NADP By similarity
	Nucleotide binding	349 – 354	6	NADP By similarity
	Region	134 – 140	7	Substrate binding By similarity
Sites
	Metal binding	291	1	Magnesium or manganese By similarity
	Metal binding	314	1	Magnesium or manganese By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	122	1	NADP By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	299	1	NADP By similarity
	Binding site	367	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	179	1	Critical for catalysis By similarity
	Site	251	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	106	1	N6-acetyllysine By similarity
	Modified residue	272	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R502-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: BBB5D9C734BD8844	FASTA	452	50,883
	10 20 30 40 50 60 MAGYLRVVRS LCRASGSRPA WAPAALTAPT SQEQTRRHYA DKRIKVAKPV VEMDGDEMTR 70 80 90 100 110 120 IIWQFIKEKL ILPHVDIQLK YFDLGLPNRD QTDDQVTIDS ALATQKYSVA VKCATITPDE 130 140 150 160 170 180 ARVEEFKLKK MWKSPNGTIR NILGGTVFRE PIICKNIPRL VPGWTKPITI GRHAHGDQYK 190 200 210 220 230 240 ATDFVADRAG TFKMVFTPKD GSGVKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYAI 250 260 270 280 290 300 QKKWPLYMST KNTILKAYDG RFKDIFQEIF DKHYKTDFDK NKIWYEHRLI DDMVAQVLKS 310 320 330 340 350 360 SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT VTRHYREHQK 370 380 390 400 410 420 GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQTLEK VCVETVESGA MTKDLAGCIH 430 440 450 GLSNVKLNEH FLNTTDFLDT IKSNLDRALG RQ

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Parietal cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169742 mRNA. Translation: BAE01823.1.
3D structure databases
SMR	Q4R502. Positions 40-452.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R502.
Enzyme and pathway databases
BRENDA	1.1.1.42. 3438.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHP_MACFA

Accession

Primary (citable) accession number: Q4R502

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)