Reviewed, UniProtKB/Swiss-Prot Q4R4T0 (RPN1_MACFA)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
    EC=2.4.1.119
Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
    Ribophorin-1
    Ribophorin I
      Short name=RPN-I

Gene names

Name:	RPN1
ORF Names:	QccE-21639, QtsA-18349

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome By similarity.
Sequence similarities	Belongs to the OST1 family.
Sequence caution	The sequence BAE02415.1 differs from that shown. Reason: Frameshift at position 397.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
PTM	Acetylation Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 607	584	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1		PRO_0000331253
Regions
	Topological domain	24 – 434	411	Lumenal Potential
	Transmembrane	435 – 455	21	Potential
	Topological domain	456 – 607	152	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	187	1	N6-acetyllysine By similarity
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	24	1	A → S in BAE02415. Ref.1
	Sequence conflict	34	1	E → G in BAE02415. Ref.1
	Sequence conflict	328	1	T → A in BAE02415. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q4R4T0-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 1B5FB9DF481A67F1	FASTA	607	68,732
	10 20 30 40 50 60 MEAPVARLFL LLLLGSWTPA PGSASSEAPP LINEDVKRTV DLSSHLAKVT AEVVLAHLGG 70 80 90 100 110 120 SSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEDNNLEV RETKIKGKSG RFFIVKLPVA 130 140 150 160 170 180 LDPGAKISVI VETVYTHVLQ PYPTQITQSE KQFVVFEGNH YFYSPYPTKT QTMRVKLASR 190 200 210 220 230 240 NVESYTKLGN PTRSEDLLDY GPFRDVPAYS QDTFKVHYEN NSPFLTITSM TRVIEVSHWG 250 260 270 280 290 300 NIAVEENVDL KHTGAVLKGP FSRYDYQRQP DSGISSIRSF KTILPAAAQD VYYRDEIGNV 310 320 330 340 350 360 STSHLLILDD SVEMEIRPRF PLFGGWKTHY IVGYNLPSYE YLYNLGDQYA LKMRFVDHVF 370 380 390 400 410 420 DEQVIDSLTV KIILPEGAKN IEIDSPYEIS RAPDELHYTY LDTFGRPVIV AYKKNLVEQH 430 440 450 460 470 480 IQDIVVHYTF NKVLMLQEPL LVVAAFYILF FTVIIYVRLD FSITKDPAAE ARMKVACITE 490 500 510 520 530 540 QVLTLVNKRI GLYRHFDETV NRYKQSRDIS TLNSGKKSLE TEHKALTSEI ALLQSRLKTE 550 560 570 580 590 600 GSDLCDRVSE MQKLDAQVKE LVLKSAVEAE RLVAGKLKKD TYIENEKLIS GKRQELVTKI DHILDAL

References

[1]

"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex and Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169814 mRNA. Translation: BAE01895.1. AB179364 mRNA. Translation: BAE02415.1. Frameshift.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R4T0.
Family and domain databases
InterPro	IPR007676. Ribophorin_I. [Graphical view]
PANTHER	PTHR21049. Ribophorin_I. 1 hit.
Pfam	PF04597. Ribophorin_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPN1_MACFA

Accession

Primary (citable) accession number: Q4R4T0
Secondary accession number(s): Q4R3A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 19, 2005
Last modified:	October 13, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)