ID   ERG25_MACFA             Reviewed;         293 AA.
AC   Q4R4Q4;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=C-4 methylsterol oxidase;
DE            EC=1.14.13.72;
DE   AltName: Full=Methylsterol monooxygenase;
GN   Name=SC4MOL; ORFNames=QtrA-11285;
OS   Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Temporal cortex;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol +
CC       NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-
CC       cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-
CC       cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-
CC       methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2
CC       H(2)O.
CC   -!- CATALYTIC ACTIVITY: 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-
CC       7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4-
CC       beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+)
CC       + H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol
CC       from lanosterol: step 3/6.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein (Probable).
CC   -!- DOMAIN: The histidine box domains may contain the active site
CC       and/or be involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AB169840; BAE01921.1; -; mRNA.
DR   HOVERGEN; Q4R4Q4; -.
DR   BRENDA; 1.14.13.72; 3438.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000254; F:C-4 methylsterol oxidase activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Iron; Lipid synthesis; Membrane; NAD;
KW   Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis;
KW   Transmembrane.
FT   CHAIN         1    293       C-4 methylsterol oxidase.
FT                                /FTId=PRO_0000249850.
FT   TRANSMEM     55     75       Potential.
FT   TRANSMEM    100    120       Potential.
FT   TRANSMEM    199    219       Potential.
FT   MOTIF       157    161       Histidine box-1.
FT   MOTIF       170    174       Histidine box-2.
FT   MOTIF       249    255       Histidine box-3.
SQ   SEQUENCE   293 AA;  35252 MW;  584AF62E7F3CC698 CRC64;
     MATNESVSIF SSASLAVEYV DSLLPENPLQ EPFKNAWNYM LNNYTKFQIA TWGSLIVHEA
     LYFSFCLPGF LFQFIPYMKK YKIQKDKPET WENQWKCFKV LLFNHFCIQL PLICGTYYFT
     EYFNIPYDWE RMPRWYFLLA RCFGCAVIED TWHYFMHRLL HHKRIYKYIH KVHHEFQAPF
     GMEAEYAHPL ETLILGTGFF IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL
     IPFYAGSRHH DFHHMNFIGN YASTFTWWDR IFGTDSQYHA YYEKKKKFEK KTE
//