Reviewed,
UniProtKB/Swiss-Prot Q4R4Q4 (ERG25_MACFA)
Last modified
October 13, 2009.
Version 26.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: C-4 methylsterol oxidase EC=1.14.13.72 Alternative name(s): Methylsterol monooxygenase | ||||
| Gene names |
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| Organism | Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey) | ||||
| Taxonomic identifier | 9541 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca |
Protein attributes
| Sequence length | 293 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O. |
| Cofactor | Iron By similarity. |
| Pathway | Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6. |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein Probable. |
| Domain | The histidine box domains may contain the active site and/or be involved in metal ion binding. |
| Sequence similarities | Belongs to the sterol desaturase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | Iron NAD |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 293 | 293 | C-4 methylsterol oxidase | PRO_0000249850 | |||||
Regions | |||||||||
| Transmembrane | 55 – 75 | 21 | Potential | ||||||
| Transmembrane | 100 – 120 | 21 | Potential | ||||||
| Transmembrane | 199 – 219 | 21 | Potential | ||||||
| Motif | 157 – 161 | 5 | Histidine box-1 | ||||||
| Motif | 170 – 174 | 5 | Histidine box-2 | ||||||
| Motif | 249 – 255 | 7 | Histidine box-3 | ||||||
Sequences
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References
| [1] | "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Temporal cortex. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB169840 mRNA. Translation: BAE01921.1. |
3D structure databases | |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | Q4R4Q4. |
Enzyme and pathway databases | |
| BRENDA | 1.14.13.72. 3438. |
Family and domain databases | |
| InterPro | IPR006694. Fatty_acid_hydroxylase. [Graphical view] |
| Pfam | PF04116. FA_hydroxylase. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ERG25_MACFA | ||||||||
| Accession | Primary (citable) accession number: Q4R4Q4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


