Reviewed, UniProtKB/Swiss-Prot Q4R4P9 (ACBG1_MACFA)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase ACSBG1
    EC=6.2.1.3
Alternative name(s):
    Acyl-CoA synthetase bubblegum family member 1

Gene names

Name:	ACSBG1
ORF Names:	QtrA-12214

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subcellular location	Cytoplasm By similarity. Cytoplasmic vesicle By similarity. Microsome By similarity. Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
Sequence caution	The sequence BAE01926.1 differs from that shown. Reason: Frameshift at position 601.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Cytoplasm Cytoplasmic vesicle Endoplasmic reticulum Microsome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Long-chain-fatty-acid--CoA ligase ACSBG1		PRO_0000315809
Regions
	Nucleotide binding	282 – 290	9	ATP By similarity
	Nucleotide binding	472 – 477	6	ATP By similarity
Sites
	Binding site	550	1	ATP By similarity
	Binding site	565	1	ATP By similarity
	Binding site	701	1	ATP By similarity
Amino acid modifications
	Modified residue	135	1	Phosphotyrosine By similarity
	Modified residue	138	1	Phosphotyrosine By similarity
	Modified residue	139	1	Phosphotyrosine By similarity
	Modified residue	658	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R4P9-1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: FD4738EC60704BF3	FASTA	724	80,998
	10 20 30 40 50 60 MPRNSGAGYG CPHGDPSMLD SRETPQESRQ DMTVGTTQEK LKTSSLTDRQ PLSKESLNHA 70 80 90 100 110 120 LKLSVPEKVN NAQWDAPEEA LWTTRADGRV RLRIDPSCPQ LPYTVHRMFY EALDKYGDFS 130 140 150 160 170 180 ALGFKCQDKW EHISYSQYYL LARRAAKGFL KLGLERAHSV AILGFNSPEW FFSAVGTVFA 190 200 210 220 230 240 GGIVTGIYTT SSPEACQYIA YDCCANVIMV DTQKQLEKIL KVWKQLPHLK AVVIYKEPPP 250 260 270 280 290 300 NKMANVYTME EFMELGNEVP EEALDAIIDT QQPNQCCVLV YTSGTTGNPK GVMLSQDNIT 310 320 330 340 350 360 WTARYGSQAG DIRPAEVQQE VVVSYLPLSH IAAQIYDLWT GIQWGAQVCF AEPDALKGSL 370 380 390 400 410 420 VNTLREVEPT SHMGVPRVWE KIMERIQEVA AQSGFIRRKM LLWAMSVTLE QNLTCPGSDL 430 440 450 460 470 480 KPFTTRLADY LVLAKVRQAL GFAKCQKNFY GAAPMTAETQ HFFLGLNIRL YAGYGLSETS 490 500 510 520 530 540 GPHFMSSPCN YRLYSSGKLV PGCRVKLVNQ DTEGIGEICL WGRTIFMGYL NMEDKTCEAI 550 560 570 580 590 600 DEEGWLHTGD AGRLDADGFL YITGRLKELI ITAGGENVPP VPIEEAVKME LPIISNAMLI 610 620 630 640 650 660 GTDQRKFLSM LLTLKCTLDP DTSDPTDNLT EQAVEFCQRV GSRATTVSEI VGKDEAVYQA 670 680 690 700 710 720 IEEGIRRVNM NAAARPYHIQ KWAILERDFS ISGGELGPTM KLKRLTVLEK YKDIIDSFYR EQKM

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Temporal cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB169845 mRNA. Translation: BAE01926.1. Frameshift.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R4P9.
Enzyme and pathway databases
BRENDA	6.2.1.3. 3438.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACBG1_MACFA

Accession

Primary (citable) accession number: Q4R4P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	November 24, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)