Reviewed, UniProtKB/Swiss-Prot Q4R4P4 (FA2H_MACFA)

Names and origin

Protein names

Recommended name:
    Fatty acid 2-hydroxylase
    EC=1.-.-.-
Alternative name(s):
    Fatty acid alpha-hydroxylase

Gene names

Name:	FA2H
Synonyms:	FAAH
ORF Names:	QtrA-13193

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids By similarity.
Cofactor	Iron By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein By similarity.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the SCS7 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Fatty acid biosynthesis Lipid synthesis Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW sphingolipid metabolic process Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Fatty acid 2-hydroxylase		PRO_0000312350
Regions
	Transmembrane	168 – 188	21	Potential
	Transmembrane	213 – 233	21	Potential
	Transmembrane	271 – 291	21	Potential
	Transmembrane	292 – 312	21	Potential
	Domain	8 – 86	79	Cytochrome b5 heme-binding
Sites
	Metal binding	43	1	Iron (heme axial ligand) By similarity
	Metal binding	69	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R4P4-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: C1FEA2286C31955A	FASTA	372	42,782
	10 20 30 40 50 60 MAPAPPPAAS FSPSEVQRRL AAGACWVRRG ARLYDLSSFV RHHPGGEQLL RARAGQDISA 70 80 90 100 110 120 DLDGPPHRHS ANARRWLEQY YVGELRGEQQ GSMENEAVAL EETQKTDPAM EPRFKVVDWD 130 140 150 160 170 180 KDLVDWQKPL LWQVGHLGEK YDEWVHQPVT RPIRLFHSDL IEGLSKTVWY SVPIIWVPLV 190 200 210 220 230 240 LYLSWSYYRT FAQGNVRLFT SFTTEYALAV PKSMFPGLFM LGIFLWSLIE YLIHRFLFHM 250 260 270 280 290 300 KPPSDSYYLI MLHFVMHGQH HKAPFDGSRL VFPPVPASLV IGVFYLCLQL ILPEAVGGTV 310 320 330 340 350 360 FAGGLLGYVL YDMTHYYLHF GSPHRGSYLY NLKAHHVKHH FAHQKSGFGI STKLWDYCFH 370 TLIPEKPHLK TQ

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Temporal cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169850 mRNA. Translation: BAE01931.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R4P4.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR006694. Fatty_acid_hydroxylase. IPR014430. Ino-phos-ceramide-B_Hydrxlase. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF04116. FA_hydroxylase. 1 hit. [Graphical view]
PIRSF	PIRSF005149. IPC-B_HD. 1 hit.
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FA2H_MACFA

Accession

Primary (citable) accession number: Q4R4P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)