Q4R4F1 (SYEM_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamate--tRNA ligase, mitochondrial
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	EARS2
ORF Names:	QtsA-10185

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 41	41	Mitochondrion Potential
	Chain	42 – 506	465	Probable glutamate--tRNA ligase, mitochondrial		PRO_0000254561
Regions
	Nucleotide binding	284 – 288	5	ATP By similarity
	Region	40 – 42	3	Glutamate binding By similarity
	Region	228 – 232	5	Glutamate binding By similarity
	Motif	45 – 53	9	"HIGH" region
	Motif	284 – 288	5	"KMSKS" region
Sites
	Binding site	50	1	ATP By similarity
	Binding site	76	1	Glutamate By similarity
	Binding site	246	1	Glutamate By similarity
	Binding site	249	1	ATP By similarity
Amino acid modifications
	Modified residue	486	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R4F1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 314400085FF645F6	FASTA	506	56,865
	10 20 30 40 50 60 MAALLRRLLQ RGRPLAASGR RVGRREARLG TGPGVAVRVR FAPSPTGFLH LGGLRTALYN 70 80 90 100 110 120 YIFAKKYQGS FILRLEDTDQ TRFVPGAAEN IENMLEWAGI PPDESPRRGG PAGPYQQSQR 130 140 150 160 170 180 LELYAQATEA LLKTGAAYPC FCSPQRLELL KKEALRNHQM PRYDNRCRNM SQEQVAQKLA 190 200 210 220 230 240 KDPKPAIRFR LEQAAPAFED LVYGWNRHDV ASVEGDPVIM KSDGFPTYHL ACVVDDHHMG 250 260 270 280 290 300 ISHVLRGSEW LISTAKHLLL YQALGWHPPH FAHLPLLLNR DGSKLSKRQG DIFLEHFAAE 310 320 330 340 350 360 GFLPDSLLDI ITNCGSGFAE NQMGRTLPEL ITQFNLTRVT CHSALLDLEK LPEFNRLHLQ 370 380 390 400 410 420 RLVNNESQRC QLVEKLQALV EEAFGSQLQN RDVLNPIYME RILLLRQGHI CRLQDLVSPV 430 440 450 460 470 480 YSYLWTRPSV GRAQLDAISE EVDVIAKRVL GLLERSGMSL TQDMLSGELK KLSEGLEGTK 490 500 HSNVMKLLRV ALSGQQALSS MFKVQG

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB178963 mRNA. Translation: BAE02014.1.
3D structure databases
HSSP	HSSP built from PDB template 1NZJ based on UniProtKB P27305.
ProteinModelPortal	Q4R4F1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG056174.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYEM_MACFA

Accession

Primary (citable) accession number: Q4R4F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program