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Q4R4F1

- SYEM_MACFA

UniProt

Q4R4F1 - SYEM_MACFA

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Protein
Probable glutamate--tRNA ligase, mitochondrial
Gene
EARS2, QtsA-10185
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501ATP By similarity
Binding sitei76 – 761Glutamate By similarity
Binding sitei246 – 2461Glutamate By similarity
Binding sitei249 – 2491ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi284 – 2885ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: UniProtKB-EC
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:EARS2
ORF Names:QtsA-10185
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Mitochondrion matrix By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141Mitochondrion Reviewed prediction
Add
BLAST
Chaini42 – 506465Probable glutamate--tRNA ligase, mitochondrialUniRule annotation
PRO_0000254561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei256 – 2561N6-succinyllysine By similarity
Modified residuei486 – 4861N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Structurei

3D structure databases

ProteinModelPortaliQ4R4F1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 423Glutamate binding By similarity
Regioni228 – 2325Glutamate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 539"HIGH" regionUniRule annotation
Motifi284 – 2885"KMSKS" regionUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG056174.
KOiK01885.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R4F1-1 [UniParc]FASTAAdd to Basket

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MAALLRRLLQ RGRPLAASGR RVGRREARLG TGPGVAVRVR FAPSPTGFLH    50
LGGLRTALYN YIFAKKYQGS FILRLEDTDQ TRFVPGAAEN IENMLEWAGI 100
PPDESPRRGG PAGPYQQSQR LELYAQATEA LLKTGAAYPC FCSPQRLELL 150
KKEALRNHQM PRYDNRCRNM SQEQVAQKLA KDPKPAIRFR LEQAAPAFED 200
LVYGWNRHDV ASVEGDPVIM KSDGFPTYHL ACVVDDHHMG ISHVLRGSEW 250
LISTAKHLLL YQALGWHPPH FAHLPLLLNR DGSKLSKRQG DIFLEHFAAE 300
GFLPDSLLDI ITNCGSGFAE NQMGRTLPEL ITQFNLTRVT CHSALLDLEK 350
LPEFNRLHLQ RLVNNESQRC QLVEKLQALV EEAFGSQLQN RDVLNPIYME 400
RILLLRQGHI CRLQDLVSPV YSYLWTRPSV GRAQLDAISE EVDVIAKRVL 450
GLLERSGMSL TQDMLSGELK KLSEGLEGTK HSNVMKLLRV ALSGQQALSS 500
MFKVQG 506
Length:506
Mass (Da):56,865
Last modified:July 19, 2005 - v1
Checksum:i314400085FF645F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB178963 mRNA. Translation: BAE02014.1.
RefSeqiNP_001270434.1. NM_001283505.1.
UniGeneiMfa.3351.

Genome annotation databases

GeneIDi101926460.
KEGGimcf:101926460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB178963 mRNA. Translation: BAE02014.1 .
RefSeqi NP_001270434.1. NM_001283505.1.
UniGenei Mfa.3351.

3D structure databases

ProteinModelPortali Q4R4F1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101926460.
KEGGi mcf:101926460.

Organism-specific databases

CTDi 124454.

Phylogenomic databases

HOVERGENi HBG056174.
KOi K01885.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiSYEM_MACFA
AccessioniPrimary (citable) accession number: Q4R4F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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