ID   GLPK2_MACFA             Reviewed;         553 AA.
AC   Q4R4D5;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=Glycerol kinase 2;
DE            Short=Glycerokinase 2;
DE            Short=GK 2;
DE            EC=2.7.1.30;
DE   AltName: Full=Glycerol kinase, testis specific 2;
DE   AltName: Full=ATP:glycerol 3-phosphotransferase;
GN   Name=GK2; ORFNames=QtsA-10839;
OS   Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Key enzyme in the regulation of glycerol uptake and
CC       metabolism (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + glycerol = ADP + sn-glycerol 3-
CC       phosphate.
CC   -!- PATHWAY: Polyol metabolism; glycerol degradation via glycerol
CC       kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane; Peripheral
CC       membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By
CC       similarity). Note=In sperm the majority of the enzyme is bound to
CC       mitochondria (By similarity).
CC   -!- SIMILARITY: Belongs to the FGGY kinase family.
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DR   EMBL; AB178979; BAE02030.1; -; mRNA.
DR   HOVERGEN; Q4R4D5; -.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004370; F:glycerol kinase activity; IEA:EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR   InterPro; IPR000577; Carb_kinase_FGGY.
DR   InterPro; IPR018485; Carb_kinase_FGGY_C.
DR   InterPro; IPR018483; Carb_kinase_FGGY_CS.
DR   InterPro; IPR018484; Carb_kinase_FGGY_N.
DR   InterPro; IPR005999; Glycerol_kin.
DR   PANTHER; PTHR10196; FGGY_kin; 1.
DR   PANTHER; PTHR10196:SF9; Glycerol_kin; 1.
DR   Pfam; PF02782; FGGY_C; 1.
DR   Pfam; PF00370; FGGY_N; 1.
DR   TIGRFAMs; TIGR01311; glycerol_kin; 1.
DR   PROSITE; PS00933; FGGY_KINASES_1; 1.
DR   PROSITE; PS00445; FGGY_KINASES_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Glycerol metabolism; Kinase; Membrane;
KW   Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding;
KW   Transferase.
FT   CHAIN         1    553       Glycerol kinase 2.
FT                                /FTId=PRO_0000343677.
FT   NP_BIND     427    431       ATP (By similarity).
FT   BINDING      20     20       Substrate (By similarity).
FT   BINDING      24     24       ATP (By similarity).
FT   BINDING      94     94       Substrate (By similarity).
FT   BINDING     148    148       Substrate (By similarity).
FT   BINDING     259    259       Substrate (By similarity).
FT   BINDING     281    281       ATP (By similarity).
FT   BINDING     326    326       ATP; via carbonyl oxygen (By similarity).
SQ   SEQUENCE   553 AA;  60705 MW;  8A6567D268217EF0 CRC64;
     MADPKTAAVG PLVGAVVQGT DSTRFLVFSS KTAELLSHHK VELTQEFPKE GWVEQDPKEI
     LQSVYECIAR TCEKLDEMNI DISNIKAVGI SNQRETTVIW DKLTGEPLYN AVVWLDLRTQ
     TTVEDLSKKI PGNSNFVKSK TGLPLSTYFS AVKLRWMLDN VRHVQKAVEE GRALFGTIDS
     WLIWSLTGGV NGGVHCTDVT NASRTMLFNI HSLEWDKELC DFFEIPMDLL PNVFSSSEIY
     GLIKTGALEG VPISGCLGDQ CAALVGQMCF QEGQAKNTYG TGCFLLCNTG RKCVFSEHGL
     LTTIAYKLGK EKPAYYALEG SVAIAGAVIR WLRDNLGIIE TSGDIEKLAK EVGTSYGCYF
     VPAFSGLYAP YWEPSARGIL CGLTQFTNKC HIAFAALEAV CFQTREILEA MNRDCGIPLR
     HLQVDGGMTN NKVLMQLQAD ILHIPVIKPF MPETTALGAA MAAGAAEGVS VWSLEPQALS
     VLRMERFEPQ IQATESEIRY ATWKKAVMKS MGWVTSQSPE SGDPSIFSSM PLGFFIVSSM
     VMLIGARYIS GMP
//