Reviewed, UniProtKB/Swiss-Prot Q4R4D5 (GLPK2_MACFA)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 2
      Short name=Glycerokinase 2
      Short name=GK 2
    EC=2.7.1.30
Alternative name(s):
    Glycerol kinase, testis specific 2
    ATP:glycerol 3-phosphotransferase 2

Gene names

Name:	GK2
ORF Names:	QtsA-10839

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Subcellular location	Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: In sperm the majority of the enzyme is bound to mitochondria By similarity.
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Glycerol kinase 2		PRO_0000343677
Regions
	Nucleotide binding	427 – 431	5	ATP By similarity
Sites
	Binding site	20	1	Substrate By similarity
	Binding site	24	1	ATP By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	281	1	ATP By similarity
	Binding site	326	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R4D5-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 8A6567D268217EF0	FASTA	553	60,705
	10 20 30 40 50 60 MADPKTAAVG PLVGAVVQGT DSTRFLVFSS KTAELLSHHK VELTQEFPKE GWVEQDPKEI 70 80 90 100 110 120 LQSVYECIAR TCEKLDEMNI DISNIKAVGI SNQRETTVIW DKLTGEPLYN AVVWLDLRTQ 130 140 150 160 170 180 TTVEDLSKKI PGNSNFVKSK TGLPLSTYFS AVKLRWMLDN VRHVQKAVEE GRALFGTIDS 190 200 210 220 230 240 WLIWSLTGGV NGGVHCTDVT NASRTMLFNI HSLEWDKELC DFFEIPMDLL PNVFSSSEIY 250 260 270 280 290 300 GLIKTGALEG VPISGCLGDQ CAALVGQMCF QEGQAKNTYG TGCFLLCNTG RKCVFSEHGL 310 320 330 340 350 360 LTTIAYKLGK EKPAYYALEG SVAIAGAVIR WLRDNLGIIE TSGDIEKLAK EVGTSYGCYF 370 380 390 400 410 420 VPAFSGLYAP YWEPSARGIL CGLTQFTNKC HIAFAALEAV CFQTREILEA MNRDCGIPLR 430 440 450 460 470 480 HLQVDGGMTN NKVLMQLQAD ILHIPVIKPF MPETTALGAA MAAGAAEGVS VWSLEPQALS 490 500 510 520 530 540 VLRMERFEPQ IQATESEIRY ATWKKAVMKS MGWVTSQSPE SGDPSIFSSM PLGFFIVSSM 550 VMLIGARYIS GMP

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB178979 mRNA. Translation: BAE02030.1.
3D structure databases
HSSP	HSSP built from PDB template 1GLJ based on UniProtKB P0A6F3.
SMR	Q4R4D5. Positions 13-516.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q4R4D5.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK2_MACFA

Accession

Primary (citable) accession number: Q4R4D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 19, 2005
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)