Reviewed, UniProtKB/Swiss-Prot Q4R4A8 (MGT4C_MACFA)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
      Short name=N-acetylglucosaminyltransferase IVc
      Short name=GnT-IVc

Gene names

Name:	MGAT4C
ORF Names:	QtsA-11376

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity) By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C		PRO_0000288597
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Signal-anchor for type II membrane protein Potential
	Topological domain	45 – 478	434	Lumenal Potential
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4R4A8-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 7E0AA1B8686800C6	FASTA	478	56,089
	10 20 30 40 50 60 MFKFHQMKHI FEILDKMRCL RKRSTVSFLG VLVIFLLFMN LYIEDSYVLE GDKQLIRETS 70 80 90 100 110 120 THQLNSERYV HTFKDLSNFS GAINVTYRYL AATPLQRKRY LTIGLSSVKR KKGNYLLETI 130 140 150 160 170 180 KSIFEQSSYE ELKEISVVVH LADFNSSWRD AMVQDITQKF AHHIIAGRLM VIHAPEEYYP 190 200 210 220 230 240 ILDGLKRNYN DPEDRVKFRS KQNVDYAFLL NFCANTSDYY VMLEDDVRCS KNFLTAIKKV 250 260 270 280 290 300 IASLEGTYWV TLEFSKLGYI GKLYHSHDLP RLAHFLLMFY QEMPCDWLLT HFRGLLAQKN 310 320 330 340 350 360 VIRFKPSLFQ HMGYYSSYKG TENKLKDDDF EEESFDIPDN PPASLYTNMN VFENYEASKA 370 380 390 400 410 420 YSSVDEYFWG KPPSTGDVFV IVFENPIIIK KIKVNTGTED RQNDILHHGA LDVGENVMPS 430 440 450 460 470 KRRRQCSTYL RLGEFKNGNF EMSGVNQKIP FDIHCMRIYV TKTQKEWLII RSISIWTS

References

[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB179007 mRNA. Translation: BAE02058.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Phylogenomic databases
HOVERGEN	Q4R4A8.
Enzyme and pathway databases
BRENDA	2.4.1.145. 3438.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4C_MACFA

Accession

Primary (citable) accession number: Q4R4A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 19, 2005
Last modified:	October 13, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)