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Protein

Exo-beta-D-glucosaminidase

Gene

gls93

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes chitosan and chitooligosaccharides with retention of anomeric configuration. Has no activity against beta-D-galactoside, beta-D-glucuronide, beta-D-mannoside, chitin, glycol chitosan, cellulose, N,N'-diacetylchitibiose and pNP-GlcNAc.2 Publications

Catalytic activityi

Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini.2 Publications

pH dependencei

Optimum pH is 4.0. Stable between pH 6.0 and 9.0.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius. Stable below 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei464 – 4641Proton donorBy similarity
Active sitei539 – 5391NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

  • chitin catabolic process Source: UniProtKB-KW
  • polysaccharide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.165. 6451.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.
mycoCLAPiGLS2A_TRIRE.

Names & Taxonomyi

Protein namesi
Recommended name:
Exo-beta-D-glucosaminidaseImported (EC:3.2.1.165)
Gene namesi
Name:gls93Imported
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

  • Secretedextracellular space 1 Publication

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Propeptidei19 – 2810Sequence analysis1 PublicationPRO_0000399048
Chaini29 – 892864Exo-beta-D-glucosaminidase1 PublicationPRO_5000052424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence analysis
Glycosylationi440 – 4401N-linked (GlcNAc...)Sequence analysis
Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence analysis
Glycosylationi578 – 5781N-linked (GlcNAc...)Sequence analysis
Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence analysis
Glycosylationi825 – 8251N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

By growth on GlcN or GlcNAc.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi51453.JGI77299.

Structurei

3D structure databases

ProteinModelPortaliQ4R1C4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2230. Eukaryota.
COG3250. LUCA.
KOiK15855.
OMAiPWLYREE.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028829. Exo-b-D-glucosamin.
IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10066:SF66. PTHR10066:SF66. 2 hits.
PfamiPF00703. Glyco_hydro_2. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4R1C4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLANAIAALL LGSGIASAAG HGSPLTSKAG HRAVIPDWDL KSSSDVSKDL
60 70 80 90 100
GGLSKPGVDT SSWYHAGTSR CTIMGCLINA GVYNDEELWY SDNLNHVNWG
110 120 130 140 150
QFSVPWVYRH EFALSPAKGK HFLLQTNGIT SKADLFFNGK QIADKEYQSG
160 170 180 190 200
AYAGRTYDIT NLAAKKNALL VQVYPTDYLY DFALGYVDWN PYPSDNGSGI
210 220 230 240 250
WRDITIKETG SVSMGPVSVL VDIDVPVEKN PARVTVRAEA QNLESHAVEF
260 270 280 290 300
NAAAVISGNS CSGEALKQTI KLAPGEKKLV QFTQTIKTPS IWWPKQWGDQ
310 320 330 340 350
PLYTAEVTFS VKGAVSDTAQ TKFGVRKVTS FVNQFNDTQY SVNGHPFQVI
360 370 380 390 400
GGGYGADMFL RWDSERFTRI VEYMLDMHQN TIRLEGKMEH PELYEICDEY
410 420 430 440 450
GLMVMPGWEC CDKWEAWAYN DELAIFPPPV WDANDYETAN YSMIHEAAMM
460 470 480 490 500
QPHPSVLTFL VGSDFWPNDE AVVLYANALK NAGWQTPIIA SASKRGFPAL
510 520 530 540 550
LGPGGMKMDG PYDWVPPNYW YDVEPSEDRL GAAFGFGSEL GAGVGTPELS
560 570 580 590 600
SLRRFLNQSD LDDLWKNPNK NLFHMSTNAS SFYNRKIYNQ GLWKRYGAPT
610 620 630 640 650
SLDDYLLKAQ MMDYEATRAQ YEGFGALWTA SRPATGVIYW MLNNAWPSLH
660 670 680 690 700
WNQFDYYLHP AGSYFGTKVG SRIEHVAYNY QKKEIWVINH SLYQTGSRNI
710 720 730 740 750
KVELIDMNGK QIAKKLVQVR TKANSGFKAM DISSDINKLS SVAFLRLVLS
760 770 780 790 800
DEKGSVLSRN VYWVTKTIDE LNWDESTWYY TPVSKFVDYT PLNTLATAQV
810 820 830 840 850
SVTTSGGKHL PGIPGSQTRT VTLENKSSVP AVFIRLTLVD SKGNDVNPVS
860 870 880 890
WSDNYVTLWP HEKLQLEVGG WDGSGDKIQI SGKNIKATTV KL
Length:892
Mass (Da):99,428
Last modified:July 19, 2005 - v1
Checksum:iAA91240261C26570
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB218755 Genomic DNA. Translation: BAD99604.1.

Genome annotation databases

KEGGiag:BAD99604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB218755 Genomic DNA. Translation: BAD99604.1.

3D structure databases

ProteinModelPortaliQ4R1C4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi51453.JGI77299.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.
mycoCLAPiGLS2A_TRIRE.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAD99604.

Phylogenomic databases

eggNOGiKOG2230. Eukaryota.
COG3250. LUCA.
KOiK15855.
OMAiPWLYREE.

Enzyme and pathway databases

BRENDAi3.2.1.165. 6451.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028829. Exo-b-D-glucosamin.
IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10066:SF66. PTHR10066:SF66. 2 hits.
PfamiPF00703. Glyco_hydro_2. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEBDG_HYPJE
AccessioniPrimary (citable) accession number: Q4R1C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 19, 2005
Last modified: May 11, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.