Q4R0W1 (DOIAD_STRRI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-deoxy-scyllo-inosamine dehydrogenase
Short name=DOIA dehydrogenase
EC=1.1.1.329

Gene names

Name:	rbmC
Synonyms:	ribE

Organism

Streptomyces ribosidificus

Taxonomic identifier

80859 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD+ or NADP+, forming 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI) By similarity.
Catalytic activity	2-deoxy-scyllo-inosamine + NAD(P)+ = 3-amino-2,3-dideoxy-scyllo-inosose + NAD(P)H.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 2-deoxystreptamine biosynthesis; 2-deoxystreptamine from D-glucose 6-phosphate: step 3/4. Antibiotic biosynthesis; ribostamycin biosynthesis.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily.

Ontologies

Keywords
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	2-deoxy-scyllo-inosamine dehydrogenase		PRO_0000234046
Sites
	Metal binding	37	1	Zinc 1; catalytic By similarity
	Metal binding	59	1	Zinc 1; catalytic By similarity
	Metal binding	89	1	Zinc 2 By similarity
	Metal binding	92	1	Zinc 2 By similarity
	Metal binding	95	1	Zinc 2 By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	144	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4R0W1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: F73E7DE1F7216481	FASTA	340	35,413
	10 20 30 40 50 60 MKALVFEAPE RALLTHRDIP DPAPGEALVR IAYNSVCGSD LSFYKGVWHG STYPVVPGHE 70 80 90 100 110 120 WSGTVVDVNG PLGAELIGTN VVGDLTVSCG TCAHCTARTP TLCEDLGELG FTRDGACAEY 130 140 150 160 170 180 MTIPVGNLRR LPDTLPLRAA CQVEPLAVAL NAVDRLGVTA GEKVAVMGAG GIGLLLAQAV 190 200 210 220 230 240 RLRGGSVTAV AEPVPERRAA ALALGVPAAV SGEPGALVEL TRTHPDAVPD VVLEASGYPT 250 260 270 280 290 300 AVQESLEAVR PGGRVGLVGY RIGETAVMAP HHIVLKVLTI RASMGPGTRF EEAIEVLASG 310 320 330 340 AVTVDALLSH EFALDDYAKA LDVALRRADS NTRSYFNLQA

References

[1]

"The ribostamycin biosynthetic gene cluster in Streptomyces ribosidificus: comparison with butirosin biosynthesis." Subba B., Kharel M.K., Lee H.C., Liou K., Kim B.-G., Sohng J.K. Mol. Cells 20:90-96(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 21294 / JCM 4923 / NBRC 13796 / NRRL B-11466.

[2]

"Analysis and comparison of biosynthetic gene clusters for the 2-deoxy-inosamine containing aminoglycoside antibiotics ribostamycin, neomycin, lividomycin, paromomycin and butirosin." Aboshanab K.M.A., Schmidt-Beissner H., Wehmeier U.F., Piepersberg W., Welzel K., Vente A. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 21294 / JCM 4923 / NBRC 13796 / NRRL B-11466.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ748131 Genomic DNA. Translation: CAG34720.1. AJ744850 Genomic DNA. Translation: CAG34039.1.
3D structure databases
ProteinModelPortal	Q4R0W1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00907; UER00923. UPA00972.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.180.10. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOIAD_STRRI

Accession

Primary (citable) accession number: Q4R0W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 19, 2005
Last modified:	May 29, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program