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Protein

ATPase family AAA domain-containing protein 5

Gene

Atad5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1119 – 11268ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase family AAA domain-containing protein 5
Alternative name(s):
Chromosome fragility-associated gene 1 protein
Gene namesi
Name:Atad5
Synonyms:Frag1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2442925. Atad5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18261826ATPase family AAA domain-containing protein 5PRO_0000317619Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei215 – 2151PhosphoserineBy similarity
Modified residuei351 – 3511PhosphoserineCombined sources
Modified residuei366 – 3661PhosphoserineBy similarity
Modified residuei603 – 6031PhosphoserineBy similarity
Modified residuei727 – 7271PhosphoserineCombined sources
Modified residuei1104 – 11041PhosphoserineBy similarity

Post-translational modificationi

ATR may stimulate the RAD9A dissociation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ4QY64.
MaxQBiQ4QY64.
PaxDbiQ4QY64.
PRIDEiQ4QY64.

PTM databases

iPTMnetiQ4QY64.
PhosphoSiteiQ4QY64.

Expressioni

Tissue specificityi

Expressed ubiquitously in all cell lines like teratocarcinoma, cell lymphoma, lymphoma.1 Publication

Inductioni

Down-regulated by DNA replication-inhibiting agents.

Gene expression databases

BgeeiQ4QY64.
CleanExiMM_ATAD5.
MM_FRAG1.
ExpressionAtlasiQ4QY64. baseline and differential.
GenevisibleiQ4QY64. MM.

Interactioni

Subunit structurei

Interacts with BRD4 (By similarity). Interacts with RB1 predominantly in G1 phase via its LXCXE motif. Interacts with RAD9A in growing cells. The interaction with RAD9A is reduced after exposure to DNA replication-inhibiting agents.By similarity1 Publication

Protein-protein interaction databases

BioGridi231918. 2 interactions.
IntActiQ4QY64. 1 interaction.
STRINGi10090.ENSMUSP00000017694.

Structurei

3D structure databases

ProteinModelPortaliQ4QY64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1415 – 14195LXCXE motif

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG1968. Eukaryota.
ENOG410YF58. LUCA.
GeneTreeiENSGT00730000111103.
HOVERGENiHBG101177.
InParanoidiQ4QY64.
OMAiLWHLKPP.
OrthoDBiEOG76X5ZB.
PhylomeDBiQ4QY64.
TreeFamiTF329112.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4QY64-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGVLSMAAA AAPLPVKDYE IEPCKKRRKD DDNSSCETIT KYLSPIGKTG
60 70 80 90 100
DKVFSPPKPS NILHYFRKTS LTTEKPQSTK AYKIKPSPPL LVGNSKDCKT
110 120 130 140 150
PLEVFSNREF KRKRKRVSLS SQLNDIKIQD ECPVEISNND SKEDGGLSDC
160 170 180 190 200
VESSASVSLY KEHVEVLAES IEDSRNQPNT KSSKKKVNPK QCTTKSDDRI
210 220 230 240 250
LRKRKRSKVT GQSESVPLAD ELSLPEDGGK DSKLTKPSLA EENDSRTHAT
260 270 280 290 300
KRADLKESTI TVSYEEFVKS HKAAKVEEIP DPAVPACVPS GPGEAVKSGS
310 320 330 340 350
EGELSGSCEP SPQLHLKTVT VLAQVHPTPP KKKGKIPPIF LKQKQPELEN
360 370 380 390 400
SLSDPENEQP VQKRKSNVVI QEGQLELAVL EAWNSEASVP KCSMEERQQF
410 420 430 440 450
MRAFRQPPPD TLKNGFKKPL EKQKDPSEKS VHEGDSSSEK IIENPNIQRV
460 470 480 490 500
SSQGCLQSHA DRGSFPKEKS KKPNKKGKKT RTTAGGNREE NIQKEKTAFS
510 520 530 540 550
LKDEQDQNSL RRSVRQKSEV LKSNALLNSE NLVCEDTAHD SVQMSLCNRN
560 570 580 590 600
KSRSSSTPTR DMVTHHRAEP GSSLEYVSTR QPIRRSLRSC STPATNALGG
610 620 630 640 650
TESEDAQDTI PVKASTPKSA RTSEKHNLYT AELIIVSSDS ESPIRMKFTR
660 670 680 690 700
ISTPKKSKKS SKKSETTEEE LTSQKKKANS TSKNISKAKE LIEEAKAFQI
710 720 730 740 750
GGSKTEETVV PLRRSSRHQA RSAKEKSPEI DDSVIVIDSS PTSIREPEKS
760 770 780 790 800
QKKLQNLNDV LGKKLNKSSK NVPGKMKIAP LFLAKRTKRA AIPVFDLDES
810 820 830 840 850
SQDSSEQTQD CDVQFKAKRD FLMSGLPDLL KRQIAKKAAA LDVYNAVSTS
860 870 880 890 900
FQRVVHVQQK DDEYWLWHLK PPSCPLLTEF KELNTKVTDL SEYVVAFGEF
910 920 930 940 950
STLNPNPRSN PAAVMMRTRK DFTKEVRNLL LEEIKCSNPE FSLEKYFPLL
960 970 980 990 1000
LKKRIEHQVL CEGHGKQASP QLQPDVSQKE TKRKQVATGN QKSKRKRQNE
1010 1020 1030 1040 1050
YSVSPEEMKG RSKDLDERIS SSCTNLDPSR DSGTEDMLWT EKYQPQNSNE
1060 1070 1080 1090 1100
LIGNELAVKK LHSWLKDWKR RAELEERHNL KGKRDEKEEG ILDLSDSTDF
1110 1120 1130 1140 1150
KGSSDDEENR LCNTVLITGP TGVGKTAAVY ACAQELGFKI FEVNASSQRS
1160 1170 1180 1190 1200
GRQILSQLKE ATQSHQVDKQ GVNSQKPCFF NNYNIGKSPK KLNSPGKVVT
1210 1220 1230 1240 1250
SPRKLPPSSP KTSGQKRALL PKTLANYFKV SSKSKSNDDV GALMGDDKGV
1260 1270 1280 1290 1300
KNSSLEQRQL IQTKSTNANN SHIKDVGAEE SNRKKATSLI LFEEVDVIFD
1310 1320 1330 1340 1350
EDAGFLNAVK TFMATTKRPV ILTTSDPTFS LVFDGCFEEI NFSIPSLLNV
1360 1370 1380 1390 1400
ASYLQVICLV ENFRTDFKDF VTLLTANACD IRKSILYLQF WIRSGGGILE
1410 1420 1430 1440 1450
ERPLSHCREN SRNTLVCSED GSDANINSKK PKRNRVALPR CDTGCAEALF
1460 1470 1480 1490 1500
GLKNIASPSQ DLLSLLKHKI TTKEEWQKLI QVLTEFHVQN IDLLHSNLEV
1510 1520 1530 1540 1550
ILPLPVHVVP DVRGAYGFPV TTQASAPASM GHLTRKQSKD QPLRKSQKRK
1560 1570 1580 1590 1600
QKKMVILDDS DLFDTGLDFS GELPSLSPAP SLSVEDNIRR DSNPEIKTQN
1610 1620 1630 1640 1650
SGFKPHSVPQ PPKTLAEKKC CMLVSHCLNS LSEFMENMSF IDALLTDPGE
1660 1670 1680 1690 1700
QNELGRSAFH WTNGRVKSGL CDEFSLENRD RWAPQSAGEL KATAEALSFT
1710 1720 1730 1740 1750
ECSSTISKAL ESLNSCKQLE RDPTNELTVC VSQRRHDACF RQSAANLDNA
1760 1770 1780 1790 1800
DKRMAVIKSV FSSRSFLTLG NKQASIIDYL PTLRNICRTE KLKEQEKNKR
1810 1820
RFLHYFEGIH LEIPEETITT LAADFP
Length:1,826
Mass (Da):203,909
Last modified:July 19, 2005 - v1
Checksum:i8830CAF65BCDDA81
GO
Isoform 2 (identifier: Q4QY64-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     775-814: KMKIAPLFLA...SEQTQDCDVQ → NWIHYCFMDT...EYSGVLSQKS
     815-1826: Missing.

Note: No experimental confirmation available.
Show »
Length:814
Mass (Da):90,207
Checksum:i84F9121DBD280768
GO

Sequence cautioni

The sequence AAH38279.2 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence CAI24765.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAI25200.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAI26037.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti330 – 3301P → H in BAE38477 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei775 – 81440KMKIA…DCDVQ → NWIHYCFMDTLLLSTVLWIT IHKCLGAIHPEYSGVLSQKS in isoform 2. 1 PublicationVSP_031098Add
BLAST
Alternative sequencei815 – 18261012Missing in isoform 2. 1 PublicationVSP_031099Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY557610 mRNA. Translation: AAT52048.1.
AK032814 mRNA. Translation: BAE20483.1.
AK053857 mRNA. Translation: BAE20694.1.
AK085211 mRNA. Translation: BAC39389.2.
AK136531 mRNA. Translation: BAE23031.1.
AK165945 mRNA. Translation: BAE38477.1.
AL591113, AL663057, AL672178 Genomic DNA. Translation: CAI24765.1. Sequence problems.
AL672178, AL591113, AL663057 Genomic DNA. Translation: CAI25200.1. Sequence problems.
AL663057, AL591113, AL672178 Genomic DNA. Translation: CAI26037.1. Sequence problems.
BC038279 mRNA. Translation: AAH38279.2. Sequence problems.
CCDSiCCDS25127.1. [Q4QY64-1]
RefSeqiNP_001025027.1. NM_001029856.2. [Q4QY64-1]
UniGeneiMm.89551.

Genome annotation databases

EnsembliENSMUST00000017694; ENSMUSP00000017694; ENSMUSG00000017550. [Q4QY64-1]
GeneIDi237877.
KEGGimmu:237877.
UCSCiuc007klf.2. mouse. [Q4QY64-2]
uc007klg.2. mouse. [Q4QY64-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY557610 mRNA. Translation: AAT52048.1.
AK032814 mRNA. Translation: BAE20483.1.
AK053857 mRNA. Translation: BAE20694.1.
AK085211 mRNA. Translation: BAC39389.2.
AK136531 mRNA. Translation: BAE23031.1.
AK165945 mRNA. Translation: BAE38477.1.
AL591113, AL663057, AL672178 Genomic DNA. Translation: CAI24765.1. Sequence problems.
AL672178, AL591113, AL663057 Genomic DNA. Translation: CAI25200.1. Sequence problems.
AL663057, AL591113, AL672178 Genomic DNA. Translation: CAI26037.1. Sequence problems.
BC038279 mRNA. Translation: AAH38279.2. Sequence problems.
CCDSiCCDS25127.1. [Q4QY64-1]
RefSeqiNP_001025027.1. NM_001029856.2. [Q4QY64-1]
UniGeneiMm.89551.

3D structure databases

ProteinModelPortaliQ4QY64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231918. 2 interactions.
IntActiQ4QY64. 1 interaction.
STRINGi10090.ENSMUSP00000017694.

PTM databases

iPTMnetiQ4QY64.
PhosphoSiteiQ4QY64.

Proteomic databases

EPDiQ4QY64.
MaxQBiQ4QY64.
PaxDbiQ4QY64.
PRIDEiQ4QY64.

Protocols and materials databases

DNASUi237877.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017694; ENSMUSP00000017694; ENSMUSG00000017550. [Q4QY64-1]
GeneIDi237877.
KEGGimmu:237877.
UCSCiuc007klf.2. mouse. [Q4QY64-2]
uc007klg.2. mouse. [Q4QY64-1]

Organism-specific databases

CTDi79915.
MGIiMGI:2442925. Atad5.

Phylogenomic databases

eggNOGiKOG1968. Eukaryota.
ENOG410YF58. LUCA.
GeneTreeiENSGT00730000111103.
HOVERGENiHBG101177.
InParanoidiQ4QY64.
OMAiLWHLKPP.
OrthoDBiEOG76X5ZB.
PhylomeDBiQ4QY64.
TreeFamiTF329112.

Miscellaneous databases

ChiTaRSiAtad5. mouse.
NextBioi383540.
PROiQ4QY64.
SOURCEiSearch...

Gene expression databases

BgeeiQ4QY64.
CleanExiMM_ATAD5.
MM_FRAG1.
ExpressionAtlasiQ4QY64. baseline and differential.
GenevisibleiQ4QY64. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Frag1, a homolog of alternative replication factor C subunits, links replication stress surveillance with apoptosis."
    Ishii H., Inageta T., Mimori K., Saito T., Sasaki H., Isobe M., Mori M., Croce C.M., Huebner K., Ozawa K., Furukawa Y.
    Proc. Natl. Acad. Sci. U.S.A. 102:9655-9660(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH RAD9A AND RB1, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-762 AND 1463-1826 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cecum, Eye, Lung, Stomach and Wolffian duct.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-472 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351 AND SER-727, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung, Spleen and Testis.

Entry informationi

Entry nameiATAD5_MOUSE
AccessioniPrimary (citable) accession number: Q4QY64
Secondary accession number(s): Q3TMG5
, Q3UW85, Q3V306, Q3V3T9, Q5SSK4, Q8BUH4, Q8CGG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 19, 2005
Last modified: March 16, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.