Reviewed, UniProtKB/Swiss-Prot Q4QSC6 (NQO8_RHOMR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 8
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit 8
    NDH-1 subunit 8

Gene names

Name:

nqo8

Organism

Rhodothermus marinus (Rhodothermus obamensis)

Taxonomic identifier

29549 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteria › Sphingobacteriales › Rhodothermaceae › Rhodothermus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NQO7-14 constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane proteinPotential.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	NADH-quinone oxidoreductase subunit 8		PRO_0000240051
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	108 – 128	21	Potential
	Transmembrane	154 – 174	21	Potential
	Transmembrane	180 – 200	21	Potential
	Transmembrane	244 – 264	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	321 – 341	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4QSC6-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 546A00803E4605A9	FASTA	341	37,374
	10 20 30 40 50 60 MDLPLYWTAL IAFLIINAML LTASVLVYAE RKISGFIQHR LGPNRVGPAG FLQPFADVVK 70 80 90 100 110 120 LLFKEDIIPA QANRFIHALA PTIMVTIAMT VPALIPFARG VVIADIDVGV LAILALTSIS 130 140 150 160 170 180 VYGITLAGWS SNSKYSLLGG LRSSAQMISY ELAMGTAVLS VILQAGSLNV SAIVEAQRDG 190 200 210 220 230 240 WAILGWHVFT NPIGALIFIV TAFAETNRLP FDLPEAEQEL VGGYHTEYSG MKFGMFFLAE 250 260 270 280 290 300 YVNLFVASFV IATLFFGGYL VPFEPLLLKA FPALEGSVLL GLLQFLSLLA KTCFFAFLYI 310 320 330 340 WVRWTFPRFK YNQLMTLGWK YLLPIGLANV ILIALGVALF S

References

[1]

"A nhaD Na+/H+ antiporter and a pcd homologues are among the Rhodothermus marinus complex I genes." Melo A.M.P., Lobo S.A.L., Sousa F.L., Fernandes A.S., Pereira M.M., Hreggvidsson G.O., Kristjansson J.K., Saraiva L.M., Teixeira M. Biochim. Biophys. Acta 1709:95-103(2005) [PubMed: 16023073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PRQ-62B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY972098 Genomic DNA. Translation: AAY42992.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. Resp_NADH_DHase_1. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. False negative. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO8_RHOMR

Accession

Primary (citable) accession number: Q4QSC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	July 19, 2005
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)