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Protein

Coiled-coil domain-containing protein 88B

Gene

Ccdc88b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a positive regulator of T-cell maturation and inflammatory function. Required for several functions of T-cells in both the CD4+ and the CD8+ compartments and this includes expression of cell surface markers of activation, proliferation, and cytokine production in response to specific or non-specific stimulation and during the course of infection with the mouse malaria parasite Plasmodium berghei (PubMed:25403443).1 Publication

GO - Biological processi

  • defense response to protozoan Source: UniProtKB
  • positive regulation of cytokine production Source: UniProtKB
  • positive regulation of T cell activation Source: UniProtKB
  • positive regulation of T cell proliferation Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Coiled-coil domain-containing protein 88B
Alternative name(s):
Hook-related protein 3
Short name:
HkRP3
Gene namesi
Name:Ccdc88b
Synonyms:Ccdc88
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1925567. Ccdc88b.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show defects in T-cell functions including impaired maturation, significantly reduced activation, reduced cell division as well as impaired cytokine production in response to specific or non-specific stimulation and during the course of infection with the mouse malaria parasite Plasmodium berghei.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14811481Coiled-coil domain-containing protein 88BPRO_0000317455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei441 – 4411PhosphoserineCombined sources
Modified residuei649 – 6491PhosphoserineCombined sources
Modified residuei1353 – 13531PhosphoserineCombined sources
Modified residuei1384 – 13841PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ4QRL3.
MaxQBiQ4QRL3.
PaxDbiQ4QRL3.
PRIDEiQ4QRL3.

PTM databases

iPTMnetiQ4QRL3.
PhosphoSiteiQ4QRL3.

Expressioni

Tissue specificityi

Abundantly expressed in immune cells, including both CD4+ and CD8+ T-cells and in myeloid cells (at protein level). Expressed specifically in spleen, bone marrow, lymph nodes and thymus.1 Publication

Gene expression databases

BgeeiQ4QRL3.
CleanExiMM_CCDC88B.
GenevisibleiQ4QRL3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109067.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili200 – 22526Sequence analysisAdd
BLAST
Coiled coili258 – 491234Sequence analysisAdd
BLAST
Coiled coili731 – 1308578Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 4810Poly-Glu

Sequence similaritiesi

Belongs to the CCDC88 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4643. Eukaryota.
ENOG410YFRJ. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000060297.
HOVERGENiHBG104809.
InParanoidiQ4QRL3.
OMAiEFQHQRY.
OrthoDBiEOG7N0C3V.
PhylomeDBiQ4QRL3.
TreeFamiTF320231.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 2 hits.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4QRL3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGAKGPRLR GFLSGSLATW ALGLAGLVGE AEESAGGTEE EEEEEEEEGA
60 70 80 90 100
LCTEKRFLRL IDGALLLRVL GIIAPSSRGG LRMVRGRDGP AACRMWNLCH
110 120 130 140 150
LWGRLRDFYQ EELQLLILSP PPDLQTMGCD PFSEEAVDEL ESILRLLLGA
160 170 180 190 200
SVQCEHRELF IRHIRGLSLD VQSELAGAIQ EVTQPGAGVV LALAGPESGE
210 220 230 240 250
LVAEELEMQL RSLTGMMSRL ARERDLGAQR LAELLLEREP AHLLLPEAPA
260 270 280 290 300
NASAEGVSHH LALQLTNAKA QLRRLRQEVE EKAEQLLDSQ AEVQGLEAEI
310 320 330 340 350
RRLRQETQAL SAQAKRAELY REEAEALRER AGRLPRLQEE LRRCREKLQA
360 370 380 390 400
AEVFKGQLEE ERVLSEALEA SKVLLEEQLE VARERSARLH ETQRENLLLR
410 420 430 440 450
TRLGEAHADL DSLRHQLEQL VEENVELELE LQRSLEPPPG SPGEASLPGA
460 470 480 490 500
APSLQDEVRE AEAGRLRAVE RENRELRGQL QMLQAQLGSQ HPLLEEQREN
510 520 530 540 550
SRQPPVPNRD PATPSALHHS PQSPACQIGG EGSESLDLPS PASYSDITRS
560 570 580 590 600
PKCSQAPDSH PELESPLQMV SQDPQTSDQA LQESDPTVET HQCLEKSGHR
610 620 630 640 650
VPLQSPIVWD PPQGPEVRIE VQELLGETGS REAPQGELVH KAQVLKQESP
660 670 680 690 700
KCRPRSAELT LREPLKDQKA LDRELELSKQ QKETGRHEQR PKGLESKLGP
710 720 730 740 750
QKPQQTSEGV PDAWSREEPT PGETLVSAIP EEQALRDEVA QLRREVAGLE
760 770 780 790 800
VKLQAQAQRL EARSAEALCL SEELAQARRT EAEAHQEAEA QAREQARLRE
810 820 830 840 850
AVDTASLELE AASREREALA EALAAAGRER RQWERDGPRL RAQVEAAEQQ
860 870 880 890 900
VQALESQVRC HLEEAEREHA EKQALREELE KAVLRGQELG DRLEHLQEEL
910 920 930 940 950
EQAALERQKF LQEQENQHQR YRHLEQRLEA ELQAASTSKE EALMELKARA
960 970 980 990 1000
LQLEEELIQL RQYPVDLATG ARAGPRTVET QNGRLIEVER NNATLVAEKA
1010 1020 1030 1040 1050
ALQGQLQHLE GQLGSLQGRA QELLLQSQRA QEHSSRLQAE KSMMEMQGQE
1060 1070 1080 1090 1100
LHRKLGVLEE EVRAARRAQE ETRGQQQALL RDHEALVQLQ RRQETELEGL
1110 1120 1130 1140 1150
LVRHRDLKAN MRALELAHRE LQGRHEQLQA QRANVEAQEV ALLAERERLM
1160 1170 1180 1190 1200
QDGHRQRGLE EELRRLQNEH ERAQMLLAEV SRERGELQGE RGELRSRLAR
1210 1220 1230 1240 1250
LELERAQLEI QSQQLRESNQ QLDLSACRLT TQCELLTQLR SAQEEENRQL
1260 1270 1280 1290 1300
LAEVQALSRE NRELLERSLE SRDHLHREQR EYLDQLNALR REKQKLVEKI
1310 1320 1330 1340 1350
MDQYRVLEPG PLPRTKKGSW LADKVKRLIR PRREGALHGG PRLGADGAGS
1360 1370 1380 1390 1400
TESLGGPLET ELPEGREADG TGSSSPAPMR RVQSSLCLGD ETLAGGQRRR
1410 1420 1430 1440 1450
LSSRFPGGRS SASFSPGDTP RQRFRQRRPG PLGAPSTHSK GSGVEWDGSI
1460 1470 1480
KTLSEHEADD TREAFQEQKP EKQFLTPSLS Q
Note: No experimental confirmation available.
Length:1,481
Mass (Da):166,608
Last modified:February 5, 2008 - v2
Checksum:i3229089E792598B8
GO
Isoform 2 (identifier: Q4QRL3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     349-359: QAAEVFKGQLE → DSWVKRVKIQQ

Note: No experimental confirmation available.
Show »
Length:1,481
Mass (Da):166,776
Checksum:i98157B55422F4D65
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei349 – 35911QAAEVFKGQLE → DSWVKRVKIQQ in isoform 2. 1 PublicationVSP_030966Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC120557 Genomic DNA. No translation available.
BC076600 mRNA. Translation: AAH76600.1.
BC151001 mRNA. Translation: AAI51002.1.
BC151009 mRNA. Translation: AAI51010.1.
CCDSiCCDS37900.1. [Q4QRL3-1]
RefSeqiNP_001074760.1. NM_001081291.1. [Q4QRL3-1]
UniGeneiMm.329596.

Genome annotation databases

EnsembliENSMUST00000113440; ENSMUSP00000109067; ENSMUSG00000047810. [Q4QRL3-1]
GeneIDi78317.
KEGGimmu:78317.
UCSCiuc008gjb.1. mouse. [Q4QRL3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC120557 Genomic DNA. No translation available.
BC076600 mRNA. Translation: AAH76600.1.
BC151001 mRNA. Translation: AAI51002.1.
BC151009 mRNA. Translation: AAI51010.1.
CCDSiCCDS37900.1. [Q4QRL3-1]
RefSeqiNP_001074760.1. NM_001081291.1. [Q4QRL3-1]
UniGeneiMm.329596.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109067.

PTM databases

iPTMnetiQ4QRL3.
PhosphoSiteiQ4QRL3.

Proteomic databases

EPDiQ4QRL3.
MaxQBiQ4QRL3.
PaxDbiQ4QRL3.
PRIDEiQ4QRL3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113440; ENSMUSP00000109067; ENSMUSG00000047810. [Q4QRL3-1]
GeneIDi78317.
KEGGimmu:78317.
UCSCiuc008gjb.1. mouse. [Q4QRL3-1]

Organism-specific databases

CTDi283234.
MGIiMGI:1925567. Ccdc88b.

Phylogenomic databases

eggNOGiKOG4643. Eukaryota.
ENOG410YFRJ. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000060297.
HOVERGENiHBG104809.
InParanoidiQ4QRL3.
OMAiEFQHQRY.
OrthoDBiEOG7N0C3V.
PhylomeDBiQ4QRL3.
TreeFamiTF320231.

Miscellaneous databases

NextBioi348677.
PROiQ4QRL3.
SOURCEiSearch...

Gene expression databases

BgeeiQ4QRL3.
CleanExiMM_CCDC88B.
GenevisibleiQ4QRL3. MM.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 2 hits.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 348-1481 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain and Eye.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-649; SER-1353 AND SER-1384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.
  5. "CCDC88B is a novel regulator of maturation and effector functions of T cells during pathological inflammation."
    Kennedy J.M., Fodil N., Torre S., Bongfen S.E., Olivier J.F., Leung V., Langlais D., Meunier C., Berghout J., Langat P., Schwartzentruber J., Majewski J., Lathrop M., Vidal S.M., Gros P.
    J. Exp. Med. 211:2519-2535(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCC88B_MOUSE
AccessioniPrimary (citable) accession number: Q4QRL3
Secondary accession number(s): B2RX63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 13, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.