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Protein

Methionine aminopeptidase 1

Gene

metap1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei202 – 2021SubstrateUniRule annotation
Metal bindingi219 – 2191Divalent metal cation 1UniRule annotation
Metal bindingi230 – 2301Divalent metal cation 1UniRule annotation
Metal bindingi230 – 2301Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi293 – 2931Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei300 – 3001SubstrateUniRule annotation
Metal bindingi326 – 3261Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi357 – 3571Divalent metal cation 1UniRule annotation
Metal bindingi357 – 3571Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiREACT_182712. Inactivation, recovery and regulation of the phototransduction cascade.

Protein family/group databases

MEROPSiM24.017.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 1UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 1UniRule annotation
Short name:
MetAP 1UniRule annotation
Alternative name(s):
Peptidase M 1UniRule annotation
Gene namesi
Name:metap1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050626-124. metap1.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytosolic ribosome Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Methionine aminopeptidase 1PRO_0000323737Add
BLAST

Proteomic databases

PRIDEiQ4QRK0.

Expressioni

Gene expression databases

BgeeiQ4QRK0.
ExpressionAtlasiQ4QRK0. differential.

Interactioni

Subunit structurei

Associates with the 60S ribosomal subunit of the 80S translational complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ4QRK0.
SMRiQ4QRK0. Positions 80-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 5244Zinc finger-like; important for proper ribosome associationUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
HOVERGENiHBG067178.
InParanoidiQ4QRK0.
KOiK01265.
PhylomeDBiQ4QRK0.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4QRK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVETRECE TEGCHSEAKL QCPTCIKLGI QGSYFCSQEC FKGSWATHKL
60 70 80 90 100
LHKKAKEDKT NDEEKNCVEK EVNTDPWPGY RYTGKLRPYY PLTPMRLVPS
110 120 130 140 150
NIQRPDYADH PLGMSESEQT MKGTSQIKIL NAEEIEGMRV VCKLAREVLD
160 170 180 190 200
IAAMMVKPGV TTEEIDHAVH LACTARNCYP SPLNYYNFPK SCCTSVNEVI
210 220 230 240 250
CHGIPDRRHL QEGDILNIDI TVYHNGYHGD LNETFFVGEV DEGAKRLVQT
260 270 280 290 300
TYECLMQAID SVKPGIRYRE LGNIIQKHAQ ANGFSVVRSY CGHGIHKLFH
310 320 330 340 350
TAPNVPHYAK NKAVGVMKPG HVFTIEPMIC EGGWQDETWP DGWTAVTRDG
360 370 380
KRSAQFEHTL LVTETGCEIL TRRLEDNGRA HFLSQM
Length:386
Mass (Da):43,567
Last modified:March 18, 2008 - v2
Checksum:i74A2F0661A5BFDAB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti275 – 2751I → T in AAH96796 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC076042 mRNA. Translation: AAH76042.1.
BC096796 mRNA. Translation: AAH96796.1.
RefSeqiNP_001020336.1. NM_001025165.1.
UniGeneiDr.77060.

Genome annotation databases

GeneIDi503783.
KEGGidre:503783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC076042 mRNA. Translation: AAH76042.1.
BC096796 mRNA. Translation: AAH96796.1.
RefSeqiNP_001020336.1. NM_001025165.1.
UniGeneiDr.77060.

3D structure databases

ProteinModelPortaliQ4QRK0.
SMRiQ4QRK0. Positions 80-384.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM24.017.

Proteomic databases

PRIDEiQ4QRK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi503783.
KEGGidre:503783.

Organism-specific databases

CTDi23173.
ZFINiZDB-GENE-050626-124. metap1.

Phylogenomic databases

eggNOGiCOG0024.
HOVERGENiHBG067178.
InParanoidiQ4QRK0.
KOiK01265.
PhylomeDBiQ4QRK0.

Enzyme and pathway databases

ReactomeiREACT_182712. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

NextBioi20866326.
PROiQ4QRK0.

Gene expression databases

BgeeiQ4QRK0.
ExpressionAtlasiQ4QRK0. differential.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiMAP11_DANRE
AccessioniPrimary (citable) accession number: Q4QRK0
Secondary accession number(s): Q6DHD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: January 7, 2015
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.