Q4QRK0 (AMPM1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 1
Short name=MAP 1
Short name=MetAP 1
EC=3.4.11.18

Alternative name(s):
Peptidase M 1

Gene names

Name:

metap1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Methionine aminopeptidase 1		PRO_0000323737
Sites
	Metal binding	219	1	Cobalt 1 By similarity
	Metal binding	230	1	Cobalt 1 By similarity
	Metal binding	230	1	Cobalt 2 By similarity
	Metal binding	293	1	Cobalt 2 By similarity
	Metal binding	326	1	Cobalt 2 By similarity
	Metal binding	357	1	Cobalt 1 By similarity
	Metal binding	357	1	Cobalt 2 By similarity
	Binding site	202	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity
Experimental info
	Sequence conflict	275	1	I → T in AAH96796. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q4QRK0 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 74A2F0661A5BFDAB	FASTA	386	43,567
	10 20 30 40 50 60 MAAVETRECE TEGCHSEAKL QCPTCIKLGI QGSYFCSQEC FKGSWATHKL LHKKAKEDKT 70 80 90 100 110 120 NDEEKNCVEK EVNTDPWPGY RYTGKLRPYY PLTPMRLVPS NIQRPDYADH PLGMSESEQT 130 140 150 160 170 180 MKGTSQIKIL NAEEIEGMRV VCKLAREVLD IAAMMVKPGV TTEEIDHAVH LACTARNCYP 190 200 210 220 230 240 SPLNYYNFPK SCCTSVNEVI CHGIPDRRHL QEGDILNIDI TVYHNGYHGD LNETFFVGEV 250 260 270 280 290 300 DEGAKRLVQT TYECLMQAID SVKPGIRYRE LGNIIQKHAQ ANGFSVVRSY CGHGIHKLFH 310 320 330 340 350 360 TAPNVPHYAK NKAVGVMKPG HVFTIEPMIC EGGWQDETWP DGWTAVTRDG KRSAQFEHTL 370 380 LVTETGCEIL TRRLEDNGRA HFLSQM

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC076042 mRNA. Translation: AAH76042.1. BC096796 mRNA. Translation: AAH96796.1.
IPI	IPI00851386.
RefSeq	NP_001020336.1. NM_001025165.1.
UniGene	Dr.77060.
3D structure databases
HSSP	HSSP built from PDB template 2B3H based on UniProtKB P53582.
ProteinModelPortal	Q4QRK0.
SMR	Q4QRK0. Positions 80-384.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4QRK0.
Protein family/group databases
MEROPS	M24.001.
Proteomic databases
PRIDE	Q4QRK0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000081144; ENSDARP00000075587; ENSDARG00000033440.
GeneID	503783.
KEGG	dre:503783.
Organism-specific databases
CTD	23173.
ZFIN	ZDB-GENE-050626-124. metap1.
Phylogenomic databases
eggNOG	fiNOG07172.
GeneTree	ENSGT00390000002284.
HOVERGEN	HBG067178.
InParanoid	Q4QRK0.
OrthoDB	EOG40S0FP.
Gene expression databases
ArrayExpress	Q4QRK0.
Bgee	Q4QRK0.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM1_DANRE

Accession

Primary (citable) accession number: Q4QRK0
Secondary accession number(s): Q6DHD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 16, 2011
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program