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Protein

Methylosome protein 50

Gene

Wdr77

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-191859. snRNP Assembly.
R-RNO-3214858. RMTs methylate histone arginines.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:Wdr77
Synonyms:Mep50
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1310479. Wdr77.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Methylosome protein 50PRO_0000331609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4QR85.
PRIDEiQ4QR85.

PTM databases

iPTMnetiQ4QR85.
PhosphoSiteiQ4QR85.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016394.
GenevisibleiQ4QR85. RN.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A, WDR77, PRMT1 and ERH. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1. Interacts with CHTOP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022571.

Structurei

3D structure databases

ProteinModelPortaliQ4QR85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati22 – 7554WD 1Add
BLAST
Repeati78 – 11639WD 2Add
BLAST
Repeati123 – 16240WD 3Add
BLAST
Repeati165 – 20541WD 4Add
BLAST
Repeati209 – 25042WD 5Add
BLAST
Repeati253 – 29341WD 6Add
BLAST
Repeati295 – 33036WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0284. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00390000010711.
HOVERGENiHBG052458.
InParanoidiQ4QR85.
KOiK13221.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4QR85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKESPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG SLLLGASSLS
60 70 80 90 100
GRCWAGSLWF FKDPSAAPNE GFCSAGVQTE AGVADLTWVG DKGILVASDS
110 120 130 140 150
GAVELWELDE NETLIVSKFC KYEHDDIVST VTVLSSGTQA VSGSKDFCIK
160 170 180 190 200
IWDLAQQMSL NSYRAHAGQV TCVAASPHRE TVFLSCSEDS RILLWDTRCP
210 220 230 240 250
KPASQMGCNA SGYLPTSLAW HPQQSEIFVF GDENGSVSLV DTKNASCTLS
260 270 280 290 300
SAVHSQCVTR LVFSPHSVPF LASLSEDCSL AVLDSSFSEV FRSRAHRDFV
310 320 330 340
RDATWSPLNH SLLTTVGWDH QVIHHVVPLE PLPAPGPDSV VE
Length:342
Mass (Da):37,076
Last modified:July 19, 2005 - v1
Checksum:iE0BC151387C5AE61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097367 mRNA. Translation: AAH97367.1.
RefSeqiNP_001008771.2. NM_001008771.3.
UniGeneiRn.14128.

Genome annotation databases

EnsembliENSRNOT00000076065; ENSRNOP00000068002; ENSRNOG00000046022.
ENSRNOT00000076654; ENSRNOP00000068445; ENSRNOG00000016394.
ENSRNOT00000086279; ENSRNOP00000075656; ENSRNOG00000016394.
GeneIDi310769.
KEGGirno:310769.
UCSCiRGD:1310479. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097367 mRNA. Translation: AAH97367.1.
RefSeqiNP_001008771.2. NM_001008771.3.
UniGeneiRn.14128.

3D structure databases

ProteinModelPortaliQ4QR85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022571.

PTM databases

iPTMnetiQ4QR85.
PhosphoSiteiQ4QR85.

Proteomic databases

PaxDbiQ4QR85.
PRIDEiQ4QR85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000076065; ENSRNOP00000068002; ENSRNOG00000046022.
ENSRNOT00000076654; ENSRNOP00000068445; ENSRNOG00000016394.
ENSRNOT00000086279; ENSRNOP00000075656; ENSRNOG00000016394.
GeneIDi310769.
KEGGirno:310769.
UCSCiRGD:1310479. rat.

Organism-specific databases

CTDi79084.
RGDi1310479. Wdr77.

Phylogenomic databases

eggNOGiKOG0284. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00390000010711.
HOVERGENiHBG052458.
InParanoidiQ4QR85.
KOiK13221.

Enzyme and pathway databases

ReactomeiR-RNO-191859. snRNP Assembly.
R-RNO-3214858. RMTs methylate histone arginines.

Miscellaneous databases

PROiQ4QR85.

Gene expression databases

BgeeiENSRNOG00000016394.
GenevisibleiQ4QR85. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP50_RAT
AccessioniPrimary (citable) accession number: Q4QR85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 19, 2005
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.