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Protein

Methylosome protein 50

Gene

Wdr77

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.By similarity

GO - Molecular functioni

Enzyme and pathway databases

ReactomeiR-RNO-3214858 RMTs methylate histone arginines

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:Wdr77
Synonyms:Mep50
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1310479 Wdr77

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003316091 – 342Methylosome protein 50Add BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4QR85
PRIDEiQ4QR85

PTM databases

iPTMnetiQ4QR85
PhosphoSitePlusiQ4QR85

Expressioni

Gene expression databases

BgeeiENSRNOG00000016394
GenevisibleiQ4QR85 RN

Interactioni

Subunit structurei

Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (By similarity). Found in a complex composed of PRMT5, WDR77 and RIOK1 (By similarity). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (By similarity). Found in a complex wit the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1. Interacts with CHTOP.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022571

Structurei

3D structure databases

ProteinModelPortaliQ4QR85
SMRiQ4QR85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati22 – 75WD 1Add BLAST54
Repeati78 – 116WD 2Add BLAST39
Repeati123 – 162WD 3Add BLAST40
Repeati165 – 205WD 4Add BLAST41
Repeati209 – 250WD 5Add BLAST42
Repeati253 – 293WD 6Add BLAST41
Repeati295 – 330WD 7Add BLAST36

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0284 Eukaryota
COG2319 LUCA
GeneTreeiENSGT00390000010711
HOVERGENiHBG052458
InParanoidiQ4QR85
KOiK13221

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 2 hits
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q4QR85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKESPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG SLLLGASSLS
60 70 80 90 100
GRCWAGSLWF FKDPSAAPNE GFCSAGVQTE AGVADLTWVG DKGILVASDS
110 120 130 140 150
GAVELWELDE NETLIVSKFC KYEHDDIVST VTVLSSGTQA VSGSKDFCIK
160 170 180 190 200
IWDLAQQMSL NSYRAHAGQV TCVAASPHRE TVFLSCSEDS RILLWDTRCP
210 220 230 240 250
KPASQMGCNA SGYLPTSLAW HPQQSEIFVF GDENGSVSLV DTKNASCTLS
260 270 280 290 300
SAVHSQCVTR LVFSPHSVPF LASLSEDCSL AVLDSSFSEV FRSRAHRDFV
310 320 330 340
RDATWSPLNH SLLTTVGWDH QVIHHVVPLE PLPAPGPDSV VE
Length:342
Mass (Da):37,076
Last modified:July 19, 2005 - v1
Checksum:iE0BC151387C5AE61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097367 mRNA Translation: AAH97367.1
RefSeqiNP_001008771.2, NM_001008771.3
XP_017446815.1, XM_017591326.1
UniGeneiRn.14128

Genome annotation databases

EnsembliENSRNOT00000076065; ENSRNOP00000068002; ENSRNOG00000046022
ENSRNOT00000076654; ENSRNOP00000068445; ENSRNOG00000016394
ENSRNOT00000086279; ENSRNOP00000075656; ENSRNOG00000016394
GeneIDi100911453
310769
KEGGirno:100911453
rno:310769
UCSCiRGD:1310479 rat

Similar proteinsi

Entry informationi

Entry nameiMEP50_RAT
AccessioniPrimary (citable) accession number: Q4QR85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 19, 2005
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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