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Protein

Cholesterol 25-hydroxylase

Gene

Ch25h

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.By similarity

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.By similarity

Cofactori

Fe cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.99.38. 5301.
ReactomeiR-RNO-192105. Synthesis of bile acids and bile salts.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase (EC:1.14.99.38)
Alternative name(s):
Cholesterol 25-monooxygenase
Gene namesi
Name:Ch25h
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1310575. Ch25h.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Transmembranei84 – 104HelicalSequence analysisAdd BLAST21
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268041 – 298Cholesterol 25-hydroxylaseAdd BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ4QQV7.

Expressioni

Tissue specificityi

Expressed in testicular macrophages at all stages, with the highest level in 10 day old animals.2 Publications

Inductioni

Down-regulated by testosterone.1 Publication

Gene expression databases

BgeeiENSRNOG00000019141.
GenevisibleiQ4QQV7. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025856.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 146Histidine box-15
Motifi157 – 161Histidine box-25
Motifi238 – 244Histidine box-37

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
HOVERGENiHBG080944.
InParanoidiQ4QQV7.
KOiK10223.
OMAiCHTTFIL.
OrthoDBiEOG091G0CY4.
PhylomeDBiQ4QQV7.
TreeFamiTF353265.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4QQV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACHNVSELQ DLGCSNQLLL QPLWDSIRTG EASARSPFFP VIFSIFTYLG
60 70 80 90 100
FCLPFVVLDV LCPWVPILRR YKIHPDFSPS VRQLLPCLGL TLYQHLVFVF
110 120 130 140 150
PVTLMHWARS PALLPREAPE LSQLLSHVLI CLLLFDTEIF AWHLLHHKVP
160 170 180 190 200
WLYRTFHKVH HQNSSSFALA TQYMSVWELL SLTFFDVLNV AMLQCHPLTI
210 220 230 240 250
LVFHVVNIWL SVEDHSGYDF PWSTHRLVPF GWYGGVAHHD LHHSQFNCNF
260 270 280 290
APYFTHWDKM LGTLRCAPHS KRLCAGSESC LDSGEQCTVH LNQKKKQT
Length:298
Mass (Da):34,414
Last modified:July 19, 2005 - v1
Checksum:i25BC9E1DDAC4AF3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097964 mRNA. Translation: AAH97964.1.
RefSeqiNP_001020586.1. NM_001025415.1.
XP_006231353.1. XM_006231291.3.
UniGeneiRn.155971.

Genome annotation databases

EnsembliENSRNOT00000025856; ENSRNOP00000025856; ENSRNOG00000019141.
GeneIDi309527.
KEGGirno:309527.
UCSCiRGD:1310575. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097964 mRNA. Translation: AAH97964.1.
RefSeqiNP_001020586.1. NM_001025415.1.
XP_006231353.1. XM_006231291.3.
UniGeneiRn.155971.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025856.

Proteomic databases

PaxDbiQ4QQV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025856; ENSRNOP00000025856; ENSRNOG00000019141.
GeneIDi309527.
KEGGirno:309527.
UCSCiRGD:1310575. rat.

Organism-specific databases

CTDi9023.
RGDi1310575. Ch25h.

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
HOVERGENiHBG080944.
InParanoidiQ4QQV7.
KOiK10223.
OMAiCHTTFIL.
OrthoDBiEOG091G0CY4.
PhylomeDBiQ4QQV7.
TreeFamiTF353265.

Enzyme and pathway databases

BRENDAi1.14.99.38. 5301.
ReactomeiR-RNO-192105. Synthesis of bile acids and bile salts.

Miscellaneous databases

PROiQ4QQV7.

Gene expression databases

BgeeiENSRNOG00000019141.
GenevisibleiQ4QQV7. RN.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCH25H_RAT
AccessioniPrimary (citable) accession number: Q4QQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.