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Q4QQV7 (CH25H_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholesterol 25-hydroxylase

EC=1.14.99.38
Alternative name(s):
Cholesterol 25-monooxygenase
Gene names
Name:Ch25h
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing By similarity. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Catalytic activity

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.

Cofactor

Iron By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in testicular macrophages at all stages, with the highest level in 10 day old animals. Ref.2 Ref.3

Induction

Down-regulated by testosterone. Ref.4

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the sterol desaturase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Cholesterol 25-hydroxylase
PRO_0000226804

Regions

Transmembrane38 – 5821Helical; Potential
Transmembrane84 – 10421Helical; Potential
Transmembrane124 – 14421Helical; Potential
Transmembrane167 – 18721Helical; Potential
Transmembrane190 – 21021Helical; Potential
Motif142 – 1465Histidine box-1
Motif157 – 1615Histidine box-2
Motif238 – 2447Histidine box-3

Amino acid modifications

Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation1631N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q4QQV7 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 25BC9E1DDAC4AF3F

FASTA29834,414
        10         20         30         40         50         60 
MACHNVSELQ DLGCSNQLLL QPLWDSIRTG EASARSPFFP VIFSIFTYLG FCLPFVVLDV 

        70         80         90        100        110        120 
LCPWVPILRR YKIHPDFSPS VRQLLPCLGL TLYQHLVFVF PVTLMHWARS PALLPREAPE 

       130        140        150        160        170        180 
LSQLLSHVLI CLLLFDTEIF AWHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSVWELL 

       190        200        210        220        230        240 
SLTFFDVLNV AMLQCHPLTI LVFHVVNIWL SVEDHSGYDF PWSTHRLVPF GWYGGVAHHD 

       250        260        270        280        290 
LHHSQFNCNF APYFTHWDKM LGTLRCAPHS KRLCAGSESC LDSGEQCTVH LNQKKKQT 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[2]"Production of 25-hydroxycholesterol by testicular macrophages and its effects on Leydig cells."
Lukyanenko Y.O., Chen J.-J., Hutson J.C.
Biol. Reprod. 64:790-796(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[3]"25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro."
Chen J.-J., Lukyanenko Y., Hutson J.C.
Biol. Reprod. 66:1336-1341(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Testosterone regulates 25-hydroxycholesterol production in testicular macrophages."
Lukyanenko Y., Chen J.-J., Hutson J.C.
Biol. Reprod. 67:1435-1438(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC097964 mRNA. Translation: AAH97964.1.
RefSeqNP_001020586.1. NM_001025415.1.
XP_006231353.1. XM_006231291.1.
XP_006231354.1. XM_006231292.1.
UniGeneRn.155971.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025856.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025856; ENSRNOP00000025856; ENSRNOG00000019141.
GeneID309527.
KEGGrno:309527.
UCSCRGD:1310575. rat.

Organism-specific databases

CTD9023.
RGD1310575. Ch25h.

Phylogenomic databases

eggNOGCOG3000.
GeneTreeENSGT00530000063017.
HOGENOMHOG000015767.
HOVERGENHBG080944.
InParanoidQ4QQV7.
KOK10223.
OMAWHVLHHK.
OrthoDBEOG7288S0.
PhylomeDBQ4QQV7.
TreeFamTF353265.

Gene expression databases

GenevestigatorQ4QQV7.

Family and domain databases

InterProIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio660932.
PROQ4QQV7.

Entry information

Entry nameCH25H_RAT
AccessionPrimary (citable) accession number: Q4QQV7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families