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Q4QQV7

- CH25H_RAT

UniProt

Q4QQV7 - CH25H_RAT

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Protein

Cholesterol 25-hydroxylase

Gene

Ch25h

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (By similarity). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.By similarity

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.

Cofactori

Fe cationBy similarity

GO - Molecular functioni

  1. cholesterol 25-hydroxylase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. cholesterol metabolic process Source: Ensembl
  2. fatty acid biosynthetic process Source: InterPro
  3. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_246878. Synthesis of bile acids and bile salts.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase (EC:1.14.99.38)
Alternative name(s):
Cholesterol 25-monooxygenase
Gene namesi
Name:Ch25h
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi1310575. Ch25h.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 5821HelicalSequence AnalysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence AnalysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence AnalysisAdd
BLAST
Transmembranei167 – 18721HelicalSequence AnalysisAdd
BLAST
Transmembranei190 – 21021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Cholesterol 25-hydroxylasePRO_0000226804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in testicular macrophages at all stages, with the highest level in 10 day old animals.2 Publications

Inductioni

Down-regulated by testosterone.1 Publication

Gene expression databases

GenevestigatoriQ4QQV7.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025856.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi142 – 1465Histidine box-1
Motifi157 – 1615Histidine box-2
Motifi238 – 2447Histidine box-3

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3000.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
HOVERGENiHBG080944.
InParanoidiQ4QQV7.
KOiK10223.
OMAiWHVLHHK.
OrthoDBiEOG7288S0.
PhylomeDBiQ4QQV7.
TreeFamiTF353265.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4QQV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACHNVSELQ DLGCSNQLLL QPLWDSIRTG EASARSPFFP VIFSIFTYLG
60 70 80 90 100
FCLPFVVLDV LCPWVPILRR YKIHPDFSPS VRQLLPCLGL TLYQHLVFVF
110 120 130 140 150
PVTLMHWARS PALLPREAPE LSQLLSHVLI CLLLFDTEIF AWHLLHHKVP
160 170 180 190 200
WLYRTFHKVH HQNSSSFALA TQYMSVWELL SLTFFDVLNV AMLQCHPLTI
210 220 230 240 250
LVFHVVNIWL SVEDHSGYDF PWSTHRLVPF GWYGGVAHHD LHHSQFNCNF
260 270 280 290
APYFTHWDKM LGTLRCAPHS KRLCAGSESC LDSGEQCTVH LNQKKKQT
Length:298
Mass (Da):34,414
Last modified:July 19, 2005 - v1
Checksum:i25BC9E1DDAC4AF3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097964 mRNA. Translation: AAH97964.1.
RefSeqiNP_001020586.1. NM_001025415.1.
XP_006231353.1. XM_006231291.2.
UniGeneiRn.155971.

Genome annotation databases

EnsembliENSRNOT00000025856; ENSRNOP00000025856; ENSRNOG00000019141.
GeneIDi309527.
KEGGirno:309527.
UCSCiRGD:1310575. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097964 mRNA. Translation: AAH97964.1 .
RefSeqi NP_001020586.1. NM_001025415.1.
XP_006231353.1. XM_006231291.2.
UniGenei Rn.155971.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025856.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025856 ; ENSRNOP00000025856 ; ENSRNOG00000019141 .
GeneIDi 309527.
KEGGi rno:309527.
UCSCi RGD:1310575. rat.

Organism-specific databases

CTDi 9023.
RGDi 1310575. Ch25h.

Phylogenomic databases

eggNOGi COG3000.
GeneTreei ENSGT00530000063017.
HOGENOMi HOG000015767.
HOVERGENi HBG080944.
InParanoidi Q4QQV7.
KOi K10223.
OMAi WHVLHHK.
OrthoDBi EOG7288S0.
PhylomeDBi Q4QQV7.
TreeFami TF353265.

Enzyme and pathway databases

Reactomei REACT_246878. Synthesis of bile acids and bile salts.

Miscellaneous databases

NextBioi 660932.
PROi Q4QQV7.

Gene expression databases

Genevestigatori Q4QQV7.

Family and domain databases

InterProi IPR006694. Fatty_acid_hydroxylase.
[Graphical view ]
Pfami PF04116. FA_hydroxylase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  2. "Production of 25-hydroxycholesterol by testicular macrophages and its effects on Leydig cells."
    Lukyanenko Y.O., Chen J.-J., Hutson J.C.
    Biol. Reprod. 64:790-796(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. "25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro."
    Chen J.-J., Lukyanenko Y., Hutson J.C.
    Biol. Reprod. 66:1336-1341(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Testosterone regulates 25-hydroxycholesterol production in testicular macrophages."
    Lukyanenko Y., Chen J.-J., Hutson J.C.
    Biol. Reprod. 67:1435-1438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCH25H_RAT
AccessioniPrimary (citable) accession number: Q4QQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 19, 2005
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3