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Q4QQV7

- CH25H_RAT

UniProt

Q4QQV7 - CH25H_RAT

Protein

Cholesterol 25-hydroxylase

Gene

Ch25h

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing By similarity. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.By similarity

    Catalytic activityi

    Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.

    Cofactori

    Iron.By similarity

    GO - Molecular functioni

    1. cholesterol 25-hydroxylase activity Source: UniProtKB-EC
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. cholesterol metabolic process Source: Ensembl
    2. fatty acid biosynthetic process Source: InterPro
    3. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholesterol 25-hydroxylase (EC:1.14.99.38)
    Alternative name(s):
    Cholesterol 25-monooxygenase
    Gene namesi
    Name:Ch25h
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1310575. Ch25h.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Cholesterol 25-hydroxylasePRO_0000226804Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in testicular macrophages at all stages, with the highest level in 10 day old animals.2 Publications

    Inductioni

    Down-regulated by testosterone.1 Publication

    Gene expression databases

    GenevestigatoriQ4QQV7.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025856.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei38 – 5821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei84 – 10421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei124 – 14421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei167 – 18721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei190 – 21021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi142 – 1465Histidine box-1
    Motifi157 – 1615Histidine box-2
    Motifi238 – 2447Histidine box-3

    Sequence similaritiesi

    Belongs to the sterol desaturase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3000.
    GeneTreeiENSGT00530000063017.
    HOGENOMiHOG000015767.
    HOVERGENiHBG080944.
    InParanoidiQ4QQV7.
    KOiK10223.
    OMAiWHVLHHK.
    OrthoDBiEOG7288S0.
    PhylomeDBiQ4QQV7.
    TreeFamiTF353265.

    Family and domain databases

    InterProiIPR006694. Fatty_acid_hydroxylase.
    [Graphical view]
    PfamiPF04116. FA_hydroxylase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4QQV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACHNVSELQ DLGCSNQLLL QPLWDSIRTG EASARSPFFP VIFSIFTYLG    50
    FCLPFVVLDV LCPWVPILRR YKIHPDFSPS VRQLLPCLGL TLYQHLVFVF 100
    PVTLMHWARS PALLPREAPE LSQLLSHVLI CLLLFDTEIF AWHLLHHKVP 150
    WLYRTFHKVH HQNSSSFALA TQYMSVWELL SLTFFDVLNV AMLQCHPLTI 200
    LVFHVVNIWL SVEDHSGYDF PWSTHRLVPF GWYGGVAHHD LHHSQFNCNF 250
    APYFTHWDKM LGTLRCAPHS KRLCAGSESC LDSGEQCTVH LNQKKKQT 298
    Length:298
    Mass (Da):34,414
    Last modified:July 19, 2005 - v1
    Checksum:i25BC9E1DDAC4AF3F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC097964 mRNA. Translation: AAH97964.1.
    RefSeqiNP_001020586.1. NM_001025415.1.
    XP_006231353.1. XM_006231291.1.
    XP_006231354.1. XM_006231292.1.
    UniGeneiRn.155971.

    Genome annotation databases

    EnsembliENSRNOT00000025856; ENSRNOP00000025856; ENSRNOG00000019141.
    GeneIDi309527.
    KEGGirno:309527.
    UCSCiRGD:1310575. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC097964 mRNA. Translation: AAH97964.1 .
    RefSeqi NP_001020586.1. NM_001025415.1.
    XP_006231353.1. XM_006231291.1.
    XP_006231354.1. XM_006231292.1.
    UniGenei Rn.155971.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025856.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025856 ; ENSRNOP00000025856 ; ENSRNOG00000019141 .
    GeneIDi 309527.
    KEGGi rno:309527.
    UCSCi RGD:1310575. rat.

    Organism-specific databases

    CTDi 9023.
    RGDi 1310575. Ch25h.

    Phylogenomic databases

    eggNOGi COG3000.
    GeneTreei ENSGT00530000063017.
    HOGENOMi HOG000015767.
    HOVERGENi HBG080944.
    InParanoidi Q4QQV7.
    KOi K10223.
    OMAi WHVLHHK.
    OrthoDBi EOG7288S0.
    PhylomeDBi Q4QQV7.
    TreeFami TF353265.

    Miscellaneous databases

    NextBioi 660932.
    PROi Q4QQV7.

    Gene expression databases

    Genevestigatori Q4QQV7.

    Family and domain databases

    InterProi IPR006694. Fatty_acid_hydroxylase.
    [Graphical view ]
    Pfami PF04116. FA_hydroxylase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    2. "Production of 25-hydroxycholesterol by testicular macrophages and its effects on Leydig cells."
      Lukyanenko Y.O., Chen J.-J., Hutson J.C.
      Biol. Reprod. 64:790-796(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    3. "25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro."
      Chen J.-J., Lukyanenko Y., Hutson J.C.
      Biol. Reprod. 66:1336-1341(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Testosterone regulates 25-hydroxycholesterol production in testicular macrophages."
      Lukyanenko Y., Chen J.-J., Hutson J.C.
      Biol. Reprod. 67:1435-1438(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiCH25H_RAT
    AccessioniPrimary (citable) accession number: Q4QQV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3