Q4QQU6 (SPF30_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Survival of motor neuron-related-splicing factor 30

Alternative name(s):
Survival motor neuron domain-containing protein 1

Gene names

Name:	Smndc1
Synonyms:	Spf30

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for spliceosome assembly. Overexpression causes apoptosis By similarity.
Subunit structure	Associates with spliceosomes. Associates with U4/U5/U6 tri-snRNP and with U2 snRNP By similarity.
Subcellular location	Nucleus speckle By similarity. Nucleus › Cajal body By similarity. Note: Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies By similarity.
Domain	The Tudor domain mediates association with dimethylarginines, which are common in snRNP proteins By similarity.
Sequence similarities	Belongs to the SMN family. Contains 1 Tudor domain.

Ontologies

Keywords
Biological process	Apoptosis mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Cajal body Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: InterPro nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q4QQU6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q4QQU6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     88-238: QCYEAEIEEI...KYNVRHLMPQ → HHDIFTGVMRRRSRRSMKRTAPLRSPSLSMAMPK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Survival of motor neuron-related-splicing factor 30		PRO_0000347224
Regions
	Domain	72 – 132	61	Tudor
	Motif	142 – 160	19	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	201	1	Phosphoserine By similarity
	Modified residue	219	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	88 – 238	151	QCYEA…HLMPQ → HHDIFTGVMRRRSRRSMKRT APLRSPSLSMAMPK in isoform 2.		VSP_035075

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 65C58A9564DE502F	FASTA	238	26,783
	10 20 30 40 50 60 MSEDLAKQLA SYKAQLQQVE AALSGNGENE DLLKLKKDLQ EVIELTKDLL STQPSETLTS 70 80 90 100 110 120 SDSFASTQPT HSWKVGDKCM AVWSEDGQCY EAEIEEIDEE NGTAAITFAV YGNAEVTPLL 130 140 150 160 170 180 NLKPVEEGRK AKEDSGNKPM SKKEMIAQQR EYKKKKALKK AQRIKELEQE REDQKVKWQQ 190 200 210 220 230 FNNRAYSKNK KGQVKRSIFA SPESVTGKVG VGTCGIADKP MTQYQDTSKY NVRHLMPQ
	Isoform 2 [UniParc]. Checksum: A52A9160BD3459B8	FASTA	121	13,522
	10 20 30 40 50 60 MSEDLAKQLA SYKAQLQQVE AALSGNGENE DLLKLKKDLQ EVIELTKDLL STQPSETLTS 70 80 90 100 110 120 SDSFASTQPT HSWKVGDKCM AVWSEDGHHD IFTGVMRRRS RRSMKRTAPL RSPSLSMAMP K

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta and Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC097986 mRNA. Translation: AAH97986.1. BC098697 mRNA. Translation: AAH98697.1.
IPI	IPI00370325. IPI00627069.
RefSeq	NP_001020571.1. NM_001025400.3. NP_001030311.1. NM_001035234.2.
UniGene	Rn.103854.
3D structure databases
ProteinModelPortal	Q4QQU6.
ModBase	Search...
Proteomic databases
PaxDb	Q4QQU6.
PRIDE	Q4QQU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000020539; ENSRNOP00000020539; ENSRNOG00000014833.
GeneID	287768.
KEGG	rno:287768.
Organism-specific databases
CTD	10285.
RGD	1309151. Smndc1.
Phylogenomic databases
eggNOG	NOG251685.
GeneTree	ENSGT00560000077236.
HOGENOM	HOG000007521.
HOVERGEN	HBG057021.
InParanoid	Q4QQU6.
KO	K12839.
OMA	NNKAYSK.
OrthoDB	EOG4H464Q.
Gene expression databases
Genevestigator	Q4QQU6.
Family and domain databases
InterPro	IPR010304. Survival_motor_neuron. IPR002999. Tudor. [Graphical view]
Pfam	PF06003. SMN. 1 hit. [Graphical view]
SMART	SM00333. TUDOR. 1 hit. [Graphical view]
PROSITE	PS50304. TUDOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	626977.

Entry information

Entry name

SPF30_RAT

Accession

Primary (citable) accession number: Q4QQU6
Secondary accession number(s): Q4KM88

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 19, 2005
Last modified:	May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program