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Protein

Probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations; Mn2+ or Mg2+. Activity higher in presence of Mn2+ than of Mg2+. Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Enzyme regulationi

The heterotetramer and the heterodimer composed of IDH3A and IDH3G subunits can be allosterically activated by citrate (CIT) or/and ADP, and the two activators can act independently or synergistically. The heterodimer composed of IDH3A and IDH3B subunits cannot be allosterically regulated and the allosteric regulation of the heterotetramer is through the IDH3G subunit and not the IDH3B subunit. The IDH3G subunit contains the allosteric site which consists of a CIT-binding site and an ADP-binding site, and the binding of CIT and ADP causes conformational changes at the allosteric site which are transmitted to the active site in the catalytic subunit (IDH3A) through a cascade of conformational changes at the heterodimer interface, leading to stabilization of the isocitrate-binding at the active site and thus activation of the enzyme. ATP can activate the heterotetramer and the heterodimer composed of IDH3A and IDH3G subunits at low concentrations but inhibits their activities at high concentrations, whereas ATP exhibits only inhibitory effect on the heterodimer composed of IDH3A and IDH3B subunits.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Allosteric activator citrateBy similarity1
Binding sitei133SubstrateBy similarity1
Binding sitei164SubstrateBy similarity1
Metal bindingi251Magnesium or manganese; shared with catalytic subunitBy similarity1
Binding sitei251SubstrateBy similarity1
Binding sitei321Allosteric activator ADPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTricarboxylic acid cycle
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial
Alternative name(s):
Isocitric dehydrogenase subunit gamma 2
NAD(+)-specific ICDH subunit gamma 2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1642415. LOC100125384.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionSequence analysisAdd BLAST25
ChainiPRO_000040193426 – 395Probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrialAdd BLAST370

Proteomic databases

PaxDbiQ4QQT5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000045720.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066907.

Structurei

3D structure databases

ProteinModelPortaliQ4QQT5.
SMRiQ4QQT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0784. Eukaryota.
COG0473. LUCA.
GeneTreeiENSGT00590000083091.
HOVERGENiHBG052080.
InParanoidiQ4QQT5.
KOiK00030.
OMAiHRNIANP.
OrthoDBiEOG091G094X.
PhylomeDBiQ4QQT5.

Family and domain databases

InterProiView protein in InterPro
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
PfamiView protein in Pfam
PF00180. Iso_dh. 1 hit.
SMARTiView protein in SMART
SM01329. Iso_dh. 1 hit.
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4QQT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAAGSCSVR TILQPALLLG HSREVVCELV TSFRNFCSKY SVPPSPKYGG
60 70 80 90 100
KHTVTMIPGD GIGPELMVHV KRIFRSNCVP VEFEEVWATS TSSEEEINNA
110 120 130 140 150
LMAIRRNRIT LKGNIATNHH LPAKYKSHNT KFRTALDLYA SVVHFKTFPG
160 170 180 190 200
VETRHKDIDI LVVRENTEGE YTNLEHESVR GVVESLKIVT KTKSVRIADY
210 220 230 240 250
AFRLAQKMGR KKVTVVHKAN IMKLGDGLFL QCCKDVAAHY PQITLESMII
260 270 280 290 300
DNTAMQLVSK PQQFDVMLMP NLYGNIINSV CTGLVGGSGI VPGANYGDSY
310 320 330 340 350
AIFETGSKEI GQDLAHRNIA NPVAMLLTSC IMLDYLDLQL YAAHIRSAVM
360 370 380 390
ASLQNKSICT PDIGGQGTTA GVVEYILDHM KDQNSGCQPR FFLST
Length:395
Mass (Da):43,568
Last modified:July 19, 2005 - v1
Checksum:iC9CCD35DC729465B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473979 Genomic DNA. Translation: EDM07215.1.
BC098006 mRNA. Translation: AAH98006.1.
RefSeqiNP_001096833.1. NM_001103363.1.
XP_008757687.1. XM_008759465.2.
UniGeneiRn.215024.

Genome annotation databases

EnsembliENSRNOT00000074027; ENSRNOP00000066907; ENSRNOG00000045720.
GeneIDi100125384.
103690168.
KEGGirno:100125384.
rno:103690168.
UCSCiRGD:1642415. rat.

Similar proteinsi

Entry informationi

Entry nameiIDHG2_RAT
AccessioniPrimary (citable) accession number: Q4QQT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: July 19, 2005
Last modified: September 27, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families