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Protein

CUGBP Elav-like family member 1

Gene

Celf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, RNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
CUGBP Elav-like family member 1
Short name:
CELF-1
Alternative name(s):
Bruno-like protein 2
CUG triplet repeat RNA-binding protein 1
Short name:
CUG-BP1
CUG-BP- and ETR-3-like factor 1
RNA-binding protein BRUNOL-2
Gene namesi
Name:Celf1
Synonyms:Cugbp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1307721. Celf1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: RNA-binding activity is detected in both nuclear and cytoplasmic compartments.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951831 – 487CUGBP Elav-like family member 1Add BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei4PhosphothreonineBy similarity1
Cross-linki109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei179PhosphoserineBy similarity1
Modified residuei303PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ4QQT3.
PeptideAtlasiQ4QQT3.
PRIDEiQ4QQT3.

PTM databases

iPTMnetiQ4QQT3.
PhosphoSitePlusiQ4QQT3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010379.
GenevisibleiQ4QQT3. RN.

Interactioni

Subunit structurei

Interacts with HNRNPH1; the interaction in RNA-dependent. Interacts with PARN. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Associates with polysomes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014484.

Structurei

3D structure databases

ProteinModelPortaliQ4QQT3.
SMRiQ4QQT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 99RRM 1PROSITE-ProRule annotationAdd BLAST84
Domaini108 – 188RRM 2PROSITE-ProRule annotationAdd BLAST81
Domaini402 – 480RRM 3PROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi265 – 274Poly-Ala10
Compositional biasi287 – 309Ser-richAdd BLAST23

Domaini

RRM1 and RRM2 domains preferentially target UGU(U/G)-rich mRNA elements.By similarity

Sequence similaritiesi

Belongs to the CELF/BRUNOL family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0144. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOGENOMiHOG000004754.
HOVERGENiHBG107646.
InParanoidiQ4QQT3.
KOiK13207.
OMAiSKCFGHT.
OrthoDBiEOG091G19O1.
PhylomeDBiQ4QQT3.

Family and domain databases

CDDicd12631. RRM1_CELF1_2_Bruno. 1 hit.
cd12634. RRM2_CELF1_2. 1 hit.
cd12638. RRM3_CELF1_2. 1 hit.
InterProiView protein in InterPro
IPR034196. CELF1/2_RRM1.
IPR034198. CELF1/2_RRM2.
IPR034199. CELF1/2_RRM3.
IPR000504. RRM_dom.
PfamiView protein in Pfam
PF00076. RRM_1. 3 hits.
SMARTiView protein in SMART
SM00360. RRM. 3 hits.
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiView protein in PROSITE
PS50102. RRM. 3 hits.

Sequencei

Sequence statusi: Complete.

Q4QQT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGTLDHPDQ PDLDAIKMFV GQVPRTWSEK DLRELFEQYG AVYEINILRD
60 70 80 90 100
RSQNPPQSKG CCFVTFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE
110 120 130 140 150
KNNAVEDRKL FIGMISKKCT ENDIRVMFSS FGQIEECRIL RGPDGLSRGC
160 170 180 190 200
AFVTFTTRTM AQTAIKAMHQ AQTMEGCSSP MVVKFADTQK DKEQKRMAQQ
210 220 230 240 250
LQQQMQQISA ASVWGNLAGL NTLGPQYLAL YLQLLQQTAN SGNLNTLSSL
260 270 280 290 300
HPMGGLNAMQ LQNLAALAAA ASAAQNTPSG TNALTTSSSP LSVLTSSAGS
310 320 330 340 350
SPSSSSSNSV NPIASLGALQ TLAGATAGLN VGSLAGMAAL NGGLGSSGLS
360 370 380 390 400
NGTGSTMEAL TQAYSGIQQY AAAALPTLYN QNLLTQQSIG AAGSQKEGPE
410 420 430 440 450
GANLFIYHLP QEFGDQDLLQ MFMPFGNVVS AKVFIDKQTN LSKCFGFVSY
460 470 480
DNPVSAQAAI QSMNGFQIGM KRLKVQLKRS KNDSKPY
Length:487
Mass (Da):52,205
Last modified:July 19, 2005 - v1
Checksum:iD39AD132660EBF40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098012 mRNA. Translation: AAH98012.1.
RefSeqiNP_001020592.1. NM_001025421.1.
XP_006234584.1. XM_006234522.2.
XP_006234585.1. XM_006234523.2.
XP_006234586.1. XM_006234524.2.
XP_008760232.1. XM_008762010.2.
XP_017447388.1. XM_017591899.1.
UniGeneiRn.22432.

Genome annotation databases

EnsembliENSRNOT00000014484; ENSRNOP00000014484; ENSRNOG00000010379.
GeneIDi362160.
KEGGirno:362160.
UCSCiRGD:1307721. rat.

Similar proteinsi

Entry informationi

Entry nameiCELF1_RAT
AccessioniPrimary (citable) accession number: Q4QQT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 19, 2005
Last modified: August 30, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families