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Q4QNJ6

- NAPA_HAEI8

UniProt

Q4QNJ6 - NAPA_HAEI8

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Protein
Periplasmic nitrate reductase
Gene
napA, NTHI0463
Organism
Haemophilus influenzae (strain 86-028NP)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S) By similarity
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHINF281310:GJ89-435-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:NTHI0463
OrganismiHaemophilus influenzae (strain 86-028NP)
Taxonomic identifieri281310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000002525: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signal Reviewed prediction
Add
BLAST
Chaini34 – 827794Periplasmic nitrate reductaseUniRule annotation
PRO_0000045988Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi281310.NTHI0463.

Structurei

3D structure databases

ProteinModelPortaliQ4QNJ6.
SMRiQ4QNJ6. Positions 37-825.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4QNJ6-1 [UniParc]FASTAAdd to Basket

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MNLSRRDFMK ANAAMAAATA AGLTIPVKNV VAAESEIKWD KAVCRFCGTG    50
CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTQPLL 100
RMTNGKFDKN GDFAPVSWDF AFKTMAEKFK EAFKKNGQNA VGMFSSGQST 150
IWEGYAKNKL WKAGFRSNNV DPNARHCMAS AAVAFMRTFG MDEPMGCYDD 200
IEQADAFVLW GSNMAEMHPI LWSRITDRRI SNPDVRVTVL STYEHRSFEL 250
ADHGLIFTPQ TDLAIMNYII NYLIQNNAIN WDFVNKHTKF KRGETNIGYG 300
LRPEHPLEKD TNRATAGKMH DSSFEELKQL VSEYTVEKVS KMSGLDKVQL 350
ETLAKLYADP TKKVVSYWTM GFNQHTRGVW VNQLIYNIHL LTGKISIPGC 400
GPFSLTGQPS ACGTAREVGS FPHRLPADLV VTNPKHRETA ERIWKLPKGT 450
VSEKVGLHTI AQDRAMNDGE MNVLWQMCNN NMQAGPNINQ ERLPGWRKEG 500
NFVIVSDPYP TVSALSADLI LPTAMWVEKE GAYGNAERRT QFWRQQVKAP 550
GEAKSDLWQL MEFAKYFTTD EMWTEDLLTQ MPEYRGKTLY EVLFKNGQVD 600
KFPLSELAEG QLNDESEYFG YYVHKGLFEE YAEFGRGHGH DLAPFDMYHK 650
AHGLRWPVVE GKETLWRYRE GYDPYVKEGE GVAFYGYPDK KAIILAVPYE 700
PPAESPDNEY DLWLSTGRVL EHWHTGTMTR RVPELHRAFP NNLVWMHPLD 750
AQARGLRHGD KIKISSRRGE MISYLDTRGR NKPPRGLVFT TFFDAGQLAN 800
SLTLDATDPI SKETDFKKCA VKVEKAA 827
Length:827
Mass (Da):93,629
Last modified:February 7, 2006 - v2
Checksum:i4D798077DCB23060
GO

Sequence cautioni

The sequence AAX87401.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000057 Genomic DNA. Translation: AAX87401.1. Different initiation.
RefSeqiYP_248061.1. NC_007146.2.

Genome annotation databases

EnsemblBacteriaiAAX87401; AAX87401; NTHI0463.
GeneIDi3429783.
KEGGihit:NTHI0463.
PATRICi20181099. VBIHaeInf100748_0426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000057 Genomic DNA. Translation: AAX87401.1 . Different initiation.
RefSeqi YP_248061.1. NC_007146.2.

3D structure databases

ProteinModelPortali Q4QNJ6.
SMRi Q4QNJ6. Positions 37-825.
ModBasei Search...

Protein-protein interaction databases

STRINGi 281310.NTHI0463.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX87401 ; AAX87401 ; NTHI0463 .
GeneIDi 3429783.
KEGGi hit:NTHI0463.
PATRICi 20181099. VBIHaeInf100748_0426.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci HINF281310:GJ89-435-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
    Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
    J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 86-028NP.

Entry informationi

Entry nameiNAPA_HAEI8
AccessioniPrimary (citable) accession number: Q4QNJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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