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Q4QNJ6 (NAPA_HAEI8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:NTHI0463
OrganismHaemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]
Taxonomic identifier281310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence caution

The sequence AAX87401.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333Tat-type signal Potential
Chain34 – 827794Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045988

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4QNJ6 [UniParc].

Last modified February 7, 2006. Version 2.
Checksum: 4D798077DCB23060

FASTA82793,629
        10         20         30         40         50         60 
MNLSRRDFMK ANAAMAAATA AGLTIPVKNV VAAESEIKWD KAVCRFCGTG CAVLVGTKDG 

        70         80         90        100        110        120 
RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTQPLL RMTNGKFDKN GDFAPVSWDF 

       130        140        150        160        170        180 
AFKTMAEKFK EAFKKNGQNA VGMFSSGQST IWEGYAKNKL WKAGFRSNNV DPNARHCMAS 

       190        200        210        220        230        240 
AAVAFMRTFG MDEPMGCYDD IEQADAFVLW GSNMAEMHPI LWSRITDRRI SNPDVRVTVL 

       250        260        270        280        290        300 
STYEHRSFEL ADHGLIFTPQ TDLAIMNYII NYLIQNNAIN WDFVNKHTKF KRGETNIGYG 

       310        320        330        340        350        360 
LRPEHPLEKD TNRATAGKMH DSSFEELKQL VSEYTVEKVS KMSGLDKVQL ETLAKLYADP 

       370        380        390        400        410        420 
TKKVVSYWTM GFNQHTRGVW VNQLIYNIHL LTGKISIPGC GPFSLTGQPS ACGTAREVGS 

       430        440        450        460        470        480 
FPHRLPADLV VTNPKHRETA ERIWKLPKGT VSEKVGLHTI AQDRAMNDGE MNVLWQMCNN 

       490        500        510        520        530        540 
NMQAGPNINQ ERLPGWRKEG NFVIVSDPYP TVSALSADLI LPTAMWVEKE GAYGNAERRT 

       550        560        570        580        590        600 
QFWRQQVKAP GEAKSDLWQL MEFAKYFTTD EMWTEDLLTQ MPEYRGKTLY EVLFKNGQVD 

       610        620        630        640        650        660 
KFPLSELAEG QLNDESEYFG YYVHKGLFEE YAEFGRGHGH DLAPFDMYHK AHGLRWPVVE 

       670        680        690        700        710        720 
GKETLWRYRE GYDPYVKEGE GVAFYGYPDK KAIILAVPYE PPAESPDNEY DLWLSTGRVL 

       730        740        750        760        770        780 
EHWHTGTMTR RVPELHRAFP NNLVWMHPLD AQARGLRHGD KIKISSRRGE MISYLDTRGR 

       790        800        810        820 
NKPPRGLVFT TFFDAGQLAN SLTLDATDPI SKETDFKKCA VKVEKAA 

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References

[1]"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 86-028NP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000057 Genomic DNA. Translation: AAX87401.1. Different initiation.
RefSeqYP_248061.1. NC_007146.2.

3D structure databases

ProteinModelPortalQ4QNJ6.
SMRQ4QNJ6. Positions 37-825.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING281310.NTHI0463.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX87401; AAX87401; NTHI0463.
GeneID3429783.
KEGGhit:NTHI0463.
PATRIC20181099. VBIHaeInf100748_0426.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycHINF281310:GJ89-435-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_HAEI8
AccessionPrimary (citable) accession number: Q4QNJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families