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Q4QNJ6

- NAPA_HAEI8

UniProt

Q4QNJ6 - NAPA_HAEI8

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus influenzae (strain 86-028NP)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciHINF281310:GJ89-435-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:NTHI0463
    OrganismiHaemophilus influenzae (strain 86-028NP)
    Taxonomic identifieri281310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000002525: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Tat-type signalUniRule annotationAdd
    BLAST
    Chaini34 – 827794Periplasmic nitrate reductasePRO_0000045988Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi281310.NTHI0463.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4QNJ6.
    SMRiQ4QNJ6. Positions 37-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4QNJ6-1 [UniParc]FASTAAdd to Basket

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    MNLSRRDFMK ANAAMAAATA AGLTIPVKNV VAAESEIKWD KAVCRFCGTG    50
    CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTQPLL 100
    RMTNGKFDKN GDFAPVSWDF AFKTMAEKFK EAFKKNGQNA VGMFSSGQST 150
    IWEGYAKNKL WKAGFRSNNV DPNARHCMAS AAVAFMRTFG MDEPMGCYDD 200
    IEQADAFVLW GSNMAEMHPI LWSRITDRRI SNPDVRVTVL STYEHRSFEL 250
    ADHGLIFTPQ TDLAIMNYII NYLIQNNAIN WDFVNKHTKF KRGETNIGYG 300
    LRPEHPLEKD TNRATAGKMH DSSFEELKQL VSEYTVEKVS KMSGLDKVQL 350
    ETLAKLYADP TKKVVSYWTM GFNQHTRGVW VNQLIYNIHL LTGKISIPGC 400
    GPFSLTGQPS ACGTAREVGS FPHRLPADLV VTNPKHRETA ERIWKLPKGT 450
    VSEKVGLHTI AQDRAMNDGE MNVLWQMCNN NMQAGPNINQ ERLPGWRKEG 500
    NFVIVSDPYP TVSALSADLI LPTAMWVEKE GAYGNAERRT QFWRQQVKAP 550
    GEAKSDLWQL MEFAKYFTTD EMWTEDLLTQ MPEYRGKTLY EVLFKNGQVD 600
    KFPLSELAEG QLNDESEYFG YYVHKGLFEE YAEFGRGHGH DLAPFDMYHK 650
    AHGLRWPVVE GKETLWRYRE GYDPYVKEGE GVAFYGYPDK KAIILAVPYE 700
    PPAESPDNEY DLWLSTGRVL EHWHTGTMTR RVPELHRAFP NNLVWMHPLD 750
    AQARGLRHGD KIKISSRRGE MISYLDTRGR NKPPRGLVFT TFFDAGQLAN 800
    SLTLDATDPI SKETDFKKCA VKVEKAA 827
    Length:827
    Mass (Da):93,629
    Last modified:February 7, 2006 - v2
    Checksum:i4D798077DCB23060
    GO

    Sequence cautioni

    The sequence AAX87401.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX87401.1. Different initiation.
    RefSeqiYP_248061.1. NC_007146.2.

    Genome annotation databases

    EnsemblBacteriaiAAX87401; AAX87401; NTHI0463.
    GeneIDi3429783.
    KEGGihit:NTHI0463.
    PATRICi20181099. VBIHaeInf100748_0426.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX87401.1 . Different initiation.
    RefSeqi YP_248061.1. NC_007146.2.

    3D structure databases

    ProteinModelPortali Q4QNJ6.
    SMRi Q4QNJ6. Positions 37-825.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 281310.NTHI0463.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX87401 ; AAX87401 ; NTHI0463 .
    GeneIDi 3429783.
    KEGGi hit:NTHI0463.
    PATRICi 20181099. VBIHaeInf100748_0426.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci HINF281310:GJ89-435-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
      Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
      J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 86-028NP.

    Entry informationi

    Entry nameiNAPA_HAEI8
    AccessioniPrimary (citable) accession number: Q4QNJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3