Q4QNC8 (THIM_HAEI8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	NTHI0540

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021513
Sites
	Binding site	43	1	Substrate; via amide nitrogen By similarity
	Binding site	119	1	ATP By similarity
	Binding site	165	1	ATP By similarity
	Binding site	192	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QNC8 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 4151D777FCBFCBD4	FASTA	265	27,774
	10 20 30 40 50 60 MLMQSIYLSK IREQNPLIHN ITNIVAANFS ANGLLALGAS PLMSANIEEM QEVPKISQVL 70 80 90 100 110 120 VINIGTLIGK DREAMLQAGK TANEVGIPVV LDPVGVGATS YRRETIRQLL AEVKFALIRG 130 140 150 160 170 180 NAGELAAIAG ETWQAKGVDA GQGEVDLKAV AEKVAQRYGC TVLISGAVDI VSDGTQTATV 190 200 210 220 230 240 HNGTSLFPKV TASGCLLSAV CAAFLAVSEG NYFSATLEAC VAYTIAGECA AQGLTTQVGQ 250 260 FQIRLLDELA ALSPETIGQR GRINE

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 86-028NP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000057 Genomic DNA. Translation: AAX87469.1.
RefSeq	YP_248129.1. NC_007146.2.
3D structure databases
HSSP	HSSP built from PDB template 1ESQ based on UniProtKB P39593.
ProteinModelPortal	Q4QNC8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4QNC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3429854.
GenomeReviews	Gene locus NTHI0540 in contig CP000057_GR.
KEGG	hit:NTHI0540.
PATRIC	20181245. VBIHaeInf100748_0497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HBG351126.
OMA	AIRGNAG.
PhylomeDB	Q4QNC8.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0540-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. ThiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_HAEI8

Accession

Primary (citable) accession number: Q4QNC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program