Reviewed, UniProtKB/Swiss-Prot Q4QN72 (HIS7_HAEI8)

Names and origin

Protein names

Recommended name:
    Histidine biosynthesis bifunctional protein hisB
Including the following 2 domains:
    1- Recommended name:
            Histidinol-phosphatase
              EC=3.1.3.15
    2- Recommended name:
            Imidazoleglycerol-phosphate dehydratase
                Short name=IGPD
              EC=4.2.1.19

Gene names

Name:	hisB
Ordered Locus Names:	NTHI0602

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O. HAMAP MF_01022 L-histidinol phosphate + H2O = L-histidinol + phosphate. HAMAP MF_01022
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9. HAMAP MF_01022 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the histidinol-phosphatase family. In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histidinol-phosphatase activity Inferred from electronic annotation. Source: HAMAP imidazoleglycerol-phosphate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Histidine biosynthesis bifunctional protein hisB HAMAP MF_01022		PRO_1000063445
Regions
	Region	1 – 173	173	Histidinol-phosphatase HAMAP MF_01022
	Region	174 – 362	189	Imidazoleglycerol-phosphate dehydratase HAMAP MF_01022

Sequences

	Sequence		Length	Mass (Da)
	Q4QN72-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: D4628788FF37B586	FASTA	362	41,189
	10 20 30 40 50 60 MQPTLFIDRD GTLIDEPKTD FQIDSLEKLK LEPKVIPALL RLKAKYRFVI VSNQDGLGTD 70 80 90 100 110 120 AFPQTNFDKP HNVMMALFES QGITFDEVLI CPHKPEENCL CRKPKIKLLD HYIRKNLFDI 130 140 150 160 170 180 DRSFVIGDRE TDVQLAENLG IRAIQYDPQK MNWDLIAEKL LGETVTNCGK RPPRFAEVIR 190 200 210 220 230 240 QTKETDIKVQ IWLDEAGVNE IKTGVGFFDH MLDQIATHGG FRMNVQCKGD LWIDEHHTVE 250 260 270 280 290 300 DTALALGQAL KQAIGDKRGI ARFGFVLPMD ECKAECALDL SGRPWIKFNA CFKRDKVGDF 310 320 330 340 350 360 STELTEHFFQ SLAFSMLATI HLNVTGNNDH HKIESLFKAF GRTLRQAIRI EGNEMPSSKG VL

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000057 Genomic DNA. Translation: AAX87525.1.
RefSeq	YP_248185.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3429912.
GenomeReviews	Gene locus NTHI0602 in contig CP000057_GR.
KEGG	hit:NTHI0602.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4QN72.
OMA	Q4QN72. MVSNQDG.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0602-MON.
Family and domain databases
HAMAP	MF_01022. [Tree]
InterPro	IPR006549. HAD-SF_hydro_IIIA. IPR005954. HisB_N. IPR006543. Histidinol-phos. IPR000807. Imidazole_glycer-P_deHydtase. IPR013954. PNK3P_central-region. [Graphical view]
PANTHER	PTHR23133:SF2. Imidazole-GPD. 1 hit.
Pfam	PF00475. IGPD. 1 hit. PF08645. PNK3P. 1 hit. [Graphical view]
ProDom	PD002282. IGPD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01662. HAD-SF-IIIA. 1 hit. TIGR01261. hisB_Nterm. 1 hit. TIGR01656. Histidinol-ppas. 1 hit.
PROSITE	PS00954. IGP_DEHYDRATASE_1. 1 hit. PS00955. IGP_DEHYDRATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS7_HAEI8

Accession

Primary (citable) accession number: Q4QN72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)