Reviewed, UniProtKB/Swiss-Prot Q4QMY5 (ASNA_HAEI8)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	NTHI0692

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017946

Sequences

	Sequence		Length	Mass (Da)
	Q4QMY5-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 3AC5C7401EA22F41	FASTA	330	37,465
	10 20 30 40 50 60 MKKTFILQQQ EISFVKNTFT QNLIEQLGII EVQGPILSQV GNGMQDNLSG IEKAVQVNVK 70 80 90 100 110 120 CIPNAIFEVV HSLAKWKRHT LARFNFKEDE GLFVHMKALR PDEDSLDPTH SVYVDQWDWE 130 140 150 160 170 180 KVIPEGRRNF AYLKETVNSI YRAIRLTELA VEARFDIPSI LPKQITFVHS EDLVKRYPDL 190 200 210 220 230 240 SSKERENAIC KEYGAVFLIG IGGKLSDGKP HDGRAPDYDD WTTESENGYK GLNGDILVWN 250 260 270 280 290 300 DQLGKAFELS SMGIRVDESA LRLQVGLTGD EDRLKMDWHQ DLLNGKLPLT IGGGIGQSRL 310 320 330 AMLLLRKKHI GEVQSSVWPK EMLEEFSNIL

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000057 Genomic DNA. Translation: AAX87612.1.
RefSeq	YP_248272.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3430000.
GenomeReviews	Gene locus NTHI0692 in contig CP000057_GR.
KEGG	hit:NTHI0692.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4QMY5.
OMA	Q4QMY5. LNDNLNG.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0692-MON.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_HAEI8

Accession

Primary (citable) accession number: Q4QMY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)