Reviewed, UniProtKB/Swiss-Prot Q4QME6 (PUR4_HAEI8)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	NTHI0909

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	1297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1297	1297	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264575
Regions
	Domain	1045 – 1297	253	Glutamine amidotransferase type-1
	Nucleotide binding	308 – 319	12	ATP Potential
Sites
	Active site	1138	1	Nucleophile By similarity
	Active site	1263	1	By similarity
	Active site	1265	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QME6-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: A9479AC1DD005291	FASTA	1,297	142,712
	10 20 30 40 50 60 MTVKTFRGSP ALSEFRLTQL QQKCQQYQLP ITSVYAEYLH FVEQKTSLVE DEIVKLQALL 70 80 90 100 110 120 HYGSMFSELK PAGYCLIVTP RVGTISSWSS KATDIAHNCG LSKVNRIERG IAYYFNIERD 130 140 150 160 170 180 LTEAELATLK DLLHDRMLET VLNHETEAAL LFTQQEPKAL TTIDILNGGR QALERANIAL 190 200 210 220 230 240 GLALADDEMD YLVESFTALK RNPQDVELYM FAQANSEHCR HKIFNADWII DGKKQDKSLF 250 260 270 280 290 300 KMIKNTFEQT PDFVLSAYKD NAAVMEGSKV GRWFPDPDGQ YRVHQEDAHI LMKVETHNHP 310 320 330 340 350 360 TAISPFPGAA TGSGGEIRDE GATGRGAKPK AGLTGFSVSN LVIPNFEQPW ENPLSKPNRI 370 380 390 400 410 420 ASALDIMIDA PLGSAAFNNE FGRPALLGYF RTYEEKVNSF AGKEVRGYHK PIMLAGGIGN 430 440 450 460 470 480 IRGEQVQKGE IPIGAKLIVL GGAAMNIGLG GGAASSMDSG KSKEDLDFAS VQRENPEMER 490 500 510 520 530 540 RCQEVIDRCW QLGEENPILF IHDVGAGGLS NAMPELVHDG RRGGKFDLRS ILCDEKGMSP 550 560 570 580 590 600 LEIWCNESQE RYVLAVAPEN LELFTALCER ERAPFAVIGE ATQAEHLILH DSHFDNNPID 610 620 630 640 650 660 LPMNVLLGKT PKMTREVLSK TVENQSLKTE GIQLKEAFHR VLRLPVVAEK TFLITIGDRS 670 680 690 700 710 720 VTGMVARDQM VGPWQIPVSD VAVTTASLDS YHGEAMAMGE RSPVALLNFS ASARLAVAEA 730 740 750 760 770 780 ITNIAGTHIG EMKRIKLSAN WMSAAGHTGE DAGLYEAVKA VGEELCPALG LTIPVGKDSM 790 800 810 820 830 840 SMKTTWIDNG EQKSVTAPLS LVISAFARVE DVRKTLTPQL RTDKGLSSLL LIDLGEGHNR 850 860 870 880 890 900 LGATALAQVY KQLGDKPADV VKVQRLKDFY NAMQTLVAED KLLAYHDRSD GGLITTLAEM 910 920 930 940 950 960 AFAGHCGVEV DISALGDNDL AVLFNEELGA VIQVADSQLE SVREVLKAHN LLGITHQLGT 970 980 990 1000 1010 1020 VTADDRFEIS RGSHKLFSEK RSELRSIWAE LTYQMQRLRD NPECAEQEFE AKKNPDDKGL 1030 1040 1050 1060 1070 1080 SAFLTYDVNE DITAPFINKG VKPTIAILRE QGVNSHYEMA AAFDRAGFNA IDVHMSDLMI 1090 1100 1110 1120 1130 1140 GRRNLAEFNA MVACGGFSYG DVLGAGGGWA KSILFNPKLH EQFSQFFINP NTLTLGVCNG 1150 1160 1170 1180 1190 1200 CQMISNLAEI IPGTENWPHF VRNKSERFEA RVSLVKINEV DSVWFAGMAG SHMPIAVSHG 1210 1220 1230 1240 1250 1260 EGQVKFKSVE QFAGLKAQGI IAAQYIDNNG SPTELYPANP NGSAEGITAI TNLDGRVAIM 1270 1280 1290 MPHPERVFRA VSNSWHPENW TEDGAWMRLF RNARMVF

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000057 Genomic DNA. Translation: AAX87801.1. Different initiation.
RefSeq	YP_248461.1.
3D structure databases
HSSP	HSSP built from PDB template 1VK3 based on UniProtKB Q9X0X3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4QME6.
Genome annotation databases
GeneID	3430220.
GenomeReviews	Gene locus NTHI0909 in contig CP000057_GR.
KEGG	hit:NTHI0909.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG335309.
PhylomeDB	Q4QME6.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0909-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_HAEI8

Accession

Primary (citable) accession number: Q4QME6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)