Reviewed, UniProtKB/Swiss-Prot Q4QMD5 (RIBB_HAEI8)

Names and origin

Protein names

Recommended name:
    3,4-dihydroxy-2-butanone 4-phosphate synthase
      Short name=DHBP synthase
    EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	NTHI0925

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 3,4-dihydroxy-2-butanone 4-phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHBP synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_1000040610
Regions
	Region	38 – 39	2	Substrate binding By similarity
	Region	151 – 155	5	Substrate binding By similarity
Sites
	Metal binding	39	1	Magnesium or manganese 1 By similarity
	Metal binding	39	1	Magnesium or manganese 2 By similarity
	Metal binding	154	1	Magnesium or manganese 2 By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Site	137	1	Essential for catalytic activity By similarity
	Site	175	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QMD5-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: D64A5C8BD9FBAF2A	FASTA	215	23,281
	10 20 30 40 50 60 MNQSILSPFG NTAEERVLNA INAFKHGTGV LVLDDEDREN EGDLIFPAET ITSEQMAKLI 70 80 90 100 110 120 RYGSGIVCLC ITDERCQQLD LPPMVEHNNS VNKTAFTVTI EAAKGVSTGV SAADRVTTIQ 130 140 150 160 170 180 TAIADNAVPT DLHRPGHVFP LRAANGGVLT RRGHTEASVD LARLAGFKEA GVICEITNDD 190 200 210 GTMARTPEIV EFAKKFGYSV LTIEDLVEYR LAHNI

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000057 Genomic DNA. Translation: AAX87812.1.
RefSeq	YP_248472.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3430234.
GenomeReviews	Gene locus NTHI0925 in contig CP000057_GR.
KEGG	hit:NTHI0925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4QMD5.
OMA	Q4QMD5. YGSGIVC.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0925-MON.
Family and domain databases
HAMAP	MF_00180. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
ProDom	PD003034. DHBP_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_HAEI8

Accession

Primary (citable) accession number: Q4QMD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)