Q4QMD5 (RIBB_HAEI8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Short name=DHBP synthase
EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	NTHI0925

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_00180
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Subunit structure	Homodimer By similarity. HAMAP MF_00180
Sequence similarities	Belongs to the DHBP synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_1000040610
Regions
	Region	38 – 39	2	Substrate binding By similarity
	Region	151 – 155	5	Substrate binding By similarity
Sites
	Metal binding	39	1	Magnesium or manganese 1 By similarity
	Metal binding	39	1	Magnesium or manganese 2 By similarity
	Metal binding	154	1	Magnesium or manganese 2 By similarity
	Binding site	43	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Site	137	1	Essential for catalytic activity By similarity
	Site	175	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QMD5 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: D64A5C8BD9FBAF2A	FASTA	215	23,281
	10 20 30 40 50 60 MNQSILSPFG NTAEERVLNA INAFKHGTGV LVLDDEDREN EGDLIFPAET ITSEQMAKLI 70 80 90 100 110 120 RYGSGIVCLC ITDERCQQLD LPPMVEHNNS VNKTAFTVTI EAAKGVSTGV SAADRVTTIQ 130 140 150 160 170 180 TAIADNAVPT DLHRPGHVFP LRAANGGVLT RRGHTEASVD LARLAGFKEA GVICEITNDD 190 200 210 GTMARTPEIV EFAKKFGYSV LTIEDLVEYR LAHNI

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 86-028NP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000057 Genomic DNA. Translation: AAX87812.1.
RefSeq	YP_248472.1. NC_007146.2.
3D structure databases
HSSP	HSSP built from PDB template 1G58 based on UniProtKB P0A7J0.
ProteinModelPortal	Q4QMD5.
SMR	Q4QMD5. Positions 1-213.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4QMD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3430234.
GenomeReviews	Gene locus NTHI0925 in contig CP000057_GR.
KEGG	hit:NTHI0925.
PATRIC	20182027. VBIHaeInf100748_0858.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0108.
HOGENOM	HBG735778.
OMA	GDMIFAA.
PhylomeDB	Q4QMD5.
ProtClustDB	PRK01792.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0925-MONOMER.
Family and domain databases
HAMAP	MF_00180. RibB. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KO	K02858.
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00506. RibB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_HAEI8

Accession

Primary (citable) accession number: Q4QMD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program