Q4QM91 (PCKA_HAEI8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	NTHI0973

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity HAMAP MF_00453.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Phosphoenolpyruvate carboxykinase [ATP] HAMAP MF_00453		PRO_0000236925
Regions
	Nucleotide binding	246 – 253	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QM91 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: FC15EC63F24D1E1D	FASTA	538	59,445
	10 20 30 40 50 60 MTDLNKVVKE LEALGIYDVK EVVYNPSYEQ LFEEETKPGL EGFEKGTLTT TGAVAVDTGI 70 80 90 100 110 120 FTGRSPKDKY IVLDEKTKDT VWWTSETAKN DNKPMNQATW QSLKDLVTNQ LSRKRLFVVD 130 140 150 160 170 180 GFCGASEHDR IAVRIVTEVA WQAHFVKNMF IRPTEEQLKN FEPDFVVMNG SKVTNPNWKE 190 200 210 220 230 240 QGLNSENFVA FNLTERIQLI GGTWYGGEMK KGMFSMMNYF LPLKGVGAMH CSANVGKDGD 250 260 270 280 290 300 VAIFFGLSGT GKTTLSTDPK RELIGDDEHG WDDVGIFNFE GGCYAKTIHL SEENEPDIYH 310 320 330 340 350 360 AIRRDALLEN VVVRSDGSVD FDDGSKTENT RVSYPIYHID NIVKPVSRAG HATKVIFLTA 370 380 390 400 410 420 DAFGVLPPVS KLTPEQTKYY FLSGFTAKLA GTERGITEPT PTFSACFGAA FLTLHPTQYA 430 440 450 460 470 480 EVLVKRMQAA GAEAYLVNTG WNGTGKRISI KDTRGIIDAI LDGSIEKAEM GELPIFNLAI 490 500 510 520 530 PKALPGVDSA ILDPRDTYAD KAQWQSKAED LAGRFVKNFV KYATNEEGKA LIAAGPKA

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 86-028NP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000057 Genomic DNA. Translation: AAX87856.1.
RefSeq	YP_248516.1. NC_007146.2.
3D structure databases
ProteinModelPortal	Q4QM91.
SMR	Q4QM91. Positions 3-537.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4QM91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3430279.
GenomeReviews	Gene locus NTHI0973 in contig CP000057_GR.
KEGG	hit:NTHI0973.
PATRIC	20182121. VBIHaeInf100748_0905.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HBG293387.
OMA	VYNPSYE.
PhylomeDB	Q4QM91.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI0973-MONOMER.
Family and domain databases
HAMAP	MF_00453. PEPCK_ATP. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KO	K01610.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. PckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_HAEI8

Accession

Primary (citable) accession number: Q4QM91

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program