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Q4QM19

- GLYA_HAEI8

UniProt

Q4QM19 - GLYA_HAEI8

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Haemophilus influenzae (strain 86-028NP)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351Pyridoxal phosphateUniRule annotation
    Binding sitei55 – 551Pyridoxal phosphateUniRule annotation
    Binding sitei57 – 571SubstrateUniRule annotation
    Binding sitei64 – 641SubstrateUniRule annotation
    Binding sitei65 – 651Pyridoxal phosphateUniRule annotation
    Binding sitei99 – 991Pyridoxal phosphateUniRule annotation
    Binding sitei121 – 1211Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei175 – 1751Pyridoxal phosphateUniRule annotation
    Binding sitei203 – 2031Pyridoxal phosphateUniRule annotation
    Binding sitei228 – 2281Pyridoxal phosphateUniRule annotation
    Binding sitei235 – 2351Pyridoxal phosphateUniRule annotation
    Binding sitei263 – 2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei363 – 3631Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciHINF281310:GJ89-1004-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:NTHI1054
    OrganismiHaemophilus influenzae (strain 86-028NP)
    Taxonomic identifieri281310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000002525: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421Serine hydroxymethyltransferasePRO_0000113584Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei229 – 2291N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi281310.NTHI1054.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4QM19.
    SMRiQ4QM19. Positions 1-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1273Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    HOGENOMiHOG000239404.
    KOiK00600.
    OMAiFKRIVDY.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4QM19-1 [UniParc]FASTAAdd to Basket

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    MFTRNMTIAD YDPVLWQAIQ DENRRQEEHI ELIASENYAS PRVMEAQGSQ    50
    FTNKYAEGYP GKRYYGGCEY ADIVEQLAID RAKELFGADY VNVQPHSGSQ 100
    ANAAVYGALI NAGDTILGMD LAHGGHLTHG AKVSFSGKIY NSVLYGITAD 150
    GLIDYEDVRQ KALECKPKLI VAGFSAYSQV VDWAKMREIA DEVGAYLFVD 200
    MAHVAGLIAA GLYLNPLPHA HVVTTTTHKT LGGPRGGLIL SSCGDEEIYK 250
    KLQSSVFPAN QGGPLVHIIA AKAVCFKEAL EPQYKEYQAN VIKNAKAMVE 300
    VFKQRGYDVV SNGTENHLFL VSFIKQGLTG KAADAALGKA NITVNKNAVP 350
    NDPQKPFVTS GIRVGTPSVT RRGFNENDVR ELAGWMCDVL DALGKENEEQ 400
    VIAETKEKVL AICKRLPVYP K 421
    Length:421
    Mass (Da):45,992
    Last modified:July 19, 2005 - v1
    Checksum:iB963B33407C6DE4C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX87928.1.
    RefSeqiWP_011272264.1. NC_007146.2.
    YP_248588.1. NC_007146.2.

    Genome annotation databases

    EnsemblBacteriaiAAX87928; AAX87928; NTHI1054.
    GeneIDi3430352.
    KEGGihit:NTHI1054.
    PATRICi20182275. VBIHaeInf100748_0982.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX87928.1 .
    RefSeqi WP_011272264.1. NC_007146.2.
    YP_248588.1. NC_007146.2.

    3D structure databases

    ProteinModelPortali Q4QM19.
    SMRi Q4QM19. Positions 1-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 281310.NTHI1054.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX87928 ; AAX87928 ; NTHI1054 .
    GeneIDi 3430352.
    KEGGi hit:NTHI1054.
    PATRICi 20182275. VBIHaeInf100748_0982.

    Phylogenomic databases

    eggNOGi COG0112.
    HOGENOMi HOG000239404.
    KOi K00600.
    OMAi FKRIVDY.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci HINF281310:GJ89-1004-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
      Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
      J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 86-028NP.

    Entry informationi

    Entry nameiGLYA_HAEI8
    AccessioniPrimary (citable) accession number: Q4QM19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3