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Q4QLY8 (SYL_HAEI8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:NTHI1090
OrganismHaemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]
Taxonomic identifier281310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152024

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4QLY8 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: E8365392CF2C16EF

FASTA86197,671
        10         20         30         40         50         60 
MQEQYRPDMI EPKVQQYWAE NKVFKAIKDE SKEKYYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPFGWD AFGLPAEGAA IKNKTAPAKW TYENIAYMKK QLQLLGFGFD 

       130        140        150        160        170        180 
WDREIATCKP EYYKWEQWFF TELYKKGLVY KKTSTVNWCP NDETVLANEQ VHEGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITDYAE QLLGGLDALP QWPDMVKTMQ RNWIGRSEGV EITFDVANTN 

       250        260        270        280        290        300 
EKVAVYTTRP DTFYGVSYLG IAAAHPLASL AAQNNSELAA FIQEAKNAKV AEADLATMEK 

       310        320        330        340        350        360 
KGMATGLFAI HPLTGEKLPI WVANFVLMHY GTGAVMAVPA HDQRDFEFAQ KYSLQIKQVI 

       370        380        390        400        410        420 
EPIADEEIDL TKQAFVEHGK LVNSAEFDGK DFDGAFNGIA DKLEKLGVGK RQVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMLTLENG DVVPAPMEDL PIILPEDVVM DGVKNPIKAD PNWAKTTLNG 

       490        500        510        520        530        540 
APALKETDTF DTFMESSWYY ARYTCPQYQN GMLDAEEANY WLPVDQYIGG IEHATMHLLY 

       550        560        570        580        590        600 
FRFFHKLLRD AGFVTSDEPA DKLLCQGMVL ADAFYYTSPT NERIWVSPTL VTLERDEKGR 

       610        620        630        640        650        660 
IIKATDPEGR ELVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGAKRFL GRVWNLVYQY QQNPAKTSLD ITALSAEQKV LRREVHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAVM ELMNKLTKAS LDSEQDRAVM AEALSAVVRM LYPITPHICF ELWQALGNES 

       790        800        810        820        830        840 
AIDTAEWVKA DEAAMVEDEK LIVVQVNGKV RGKVTVAADA DEDTVKTIAF ADENVKKFID 

       850        860 
NQHIVKVIYV VGKLLNVVVK P 

« Hide

References

[1]"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 86-028NP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000057 Genomic DNA. Translation: AAX87959.1.
RefSeqYP_248619.1. NC_007146.2.

3D structure databases

ProteinModelPortalQ4QLY8.
SMRQ4QLY8. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING281310.NTHI1090.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX87959; AAX87959; NTHI1090.
GeneID3430383.
KEGGhit:NTHI1090.
PATRIC20182339. VBIHaeInf100748_1014.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAMAFAGPP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHINF281310:GJ89-1035-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HAEI8
AccessionPrimary (citable) accession number: Q4QLY8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 19, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries