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Q4QLU5

- SYI_HAEI8

UniProt

Q4QLU5 - SYI_HAEI8

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haemophilus influenzae (strain 86-028NP)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
    Binding sitei606 – 6061ATPUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHINF281310:GJ89-1079-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:NTHI1135
    OrganismiHaemophilus influenzae (strain 86-028NP)
    Taxonomic identifieri281310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000002525: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 941941Isoleucine--tRNA ligasePRO_0000098396Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi281310.NTHI1135.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4QLU5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi603 – 6075"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4QLU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVDYKNTLN LPETSFPMRG DLAKREPDML KNWYEKNLYQ KIRKASKGKK    50
    SFILHDGPPY ANGNIHIGHA VNKILKDIII KSKTALGFDS PYIPGWDCHG 100
    LPIELKVEGL VGKPNEKISA AEFRQKCREY ASEQVEGQKK DFIRLGVLGD 150
    WDNPYLTMNF DTEANIIRTL GKVIENGHLY KGSKPVHWCL DCGSSLAEAE 200
    VEYEDKVSPS IYVRFPAESA VEIEAKFSAQ GRGQGKLSAI IWTTTPWTMP 250
    SNRAIAVNAD LEYNLVQLGD ERVILAAELV ESVAKAVGVE QVEILGSVKG 300
    ADLELSRFHH PFYDFTVPVI LGDHVTTDGG TGLVHTAPDH GLDDFIVGKQ 350
    YDLPMAGLVS NDGKFISTTE FFAGKGVFEA NPLVIEKLQE VGNLLKVEKI 400
    KHSYPHCWRH KTPIIFRATP QWFIGMETQG LRQQALGEIK QVRWIPDWGQ 450
    ARIEKMVENR PDWCISRQRT WGVPMTLFVH KETEELHPRT LELLEEVAKR 500
    VERAGIQAWW DLDEKELLGA DAETYRKVPD TLDVWFDSGS TYSSVVANRL 550
    EFNGQDIDMY LEGSDQHRGW FMSSLMLSTA TNSKAPYKQV LTHGFTVDGQ 600
    GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEMT VSDEILKRAA 650
    DSYRRIRNTA RFLLANLNGF DPKRDLVKPE EMISLDRWAV ACALDAQNEI 700
    KDAYDNYQFH TVVQRLMRFC SVEMGSFYLD IIKDRQYTTK ADSLARRSCQ 750
    TALWHIAEAL VRWMAPILSF TADEIWQHLP QTESARAEFV FTEEFYQGLF 800
    GLGEDEKLDD AYWQQLIKVR SEVNRVLEIS RNNKEIGGGL EAEVTVYAND 850
    EYRALLAQLG NELRFVLITS KVDVKSLSEK PADLADSELE GIAVSVTRSN 900
    AEKCPRCWHY SDEIGVSPGH PTLCARCVEN VVGNGEVRYF A 941
    Length:941
    Mass (Da):106,393
    Last modified:July 19, 2005 - v1
    Checksum:i26003BA8A703EF91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX88002.1.
    RefSeqiYP_248662.1. NC_007146.2.

    Genome annotation databases

    EnsemblBacteriaiAAX88002; AAX88002; NTHI1135.
    GeneIDi3430427.
    KEGGihit:NTHI1135.
    PATRICi20182427. VBIHaeInf100748_1057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX88002.1 .
    RefSeqi YP_248662.1. NC_007146.2.

    3D structure databases

    ProteinModelPortali Q4QLU5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 281310.NTHI1135.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX88002 ; AAX88002 ; NTHI1135 .
    GeneIDi 3430427.
    KEGGi hit:NTHI1135.
    PATRICi 20182427. VBIHaeInf100748_1057.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HINF281310:GJ89-1079-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
      Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
      J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 86-028NP.

    Entry informationi

    Entry nameiSYI_HAEI8
    AccessioniPrimary (citable) accession number: Q4QLU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3