Reviewed, UniProtKB/Swiss-Prot Q4QK64 (SYQ_HAEI8)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	NTHI1812

Organism

Haemophilus influenzae (strain 86-028NP) [Complete proteome] [HAMAP]

Taxonomic identifier

281310 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000242870
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00126
	Motif	277 – 281	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	280	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QK64-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 6C5C1522E9A6D351	FASTA	557	64,020
	10 20 30 40 50 60 MMSHTETSLG AENTRTHNFI TQIIDEDLAS GKHKSVHTRF PPEPNGYLHI GHAKSICLNF 70 80 90 100 110 120 GLAKEYQGLC NLRFDDTNPV KEDVEYVDSI KADVEWLGFK WEGEPRYASD YFDALYGYAA 130 140 150 160 170 180 ELIKKGLAYV DELSPDEMRE YRGTLTEPGK NSPYRDRTIE ENLALFEKMK NGEFAEGKAS 190 200 210 220 230 240 LRAKIDMASP FMVMRDPVIY RIKFASHHQT GDKWCIYPMY DFTHCISDAI ERITHSICTL 250 260 270 280 290 300 EFQDNRRLYD WVLENISIER PLPHQYEFSR LNLEGTLTSK RKLLKLVNDE IVDGWNDPRM 310 320 330 340 350 360 PTISGLRRRG YTPASLREFC RRIGVTKQDN VVEYSALEAC IREDLNENAP RAMAVIDPVR 370 380 390 400 410 420 VVIENFESEA VLTAPNHPNR PELGERQLPF TKELYIDRAD FREEANKQYK RLVLGKEVRL 430 440 450 460 470 480 RNAYVIKAER VEKDANGEIT TIFCTYDPET LGKNPADGRK VKGVIHWVSA VNNHPAEFRL 490 500 510 520 530 540 YDRLFTVPNP GAEDDIESVL NPNSLVIKQG FVEQSLANAE AEKGYQFERE GYFCADSKDS 550 RPEHLVFNLT VSLKEGF

References

[1]

"Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20." Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr. J. Bacteriol. 187:4627-4636(2005) [PubMed: 15968074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000057 Genomic DNA. Translation: AAX88583.1.
RefSeq	YP_249243.1.
3D structure databases
SMR	Q4QK64. Positions 18-555.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4QK64.
Genome annotation databases
GeneID	3431025.
GenomeReviews	Gene locus NTHI1812 in contig CP000057_GR.
KEGG	hit:NTHI1812.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4QK64.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	HINF281310:NTHI1812-MON.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_HAEI8

Accession

Primary (citable) accession number: Q4QK64

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 19, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)