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Q4QJM6

- GLND_HAEI8

UniProt

Q4QJM6 - GLND_HAEI8

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Haemophilus influenzae (strain 86-028NP)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciHINF281310:GJ89-1871-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:NTHI2026
OrganismiHaemophilus influenzae (strain 86-028NP)
Taxonomic identifieri281310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000002525: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192737Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi281310.NTHI2026.

Structurei

3D structure databases

ProteinModelPortaliQ4QJM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini446 – 579134HDUniRule annotationAdd
BLAST
Domaini688 – 77285ACT 1UniRule annotationAdd
BLAST
Domaini794 – 86370ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 328328UridylyltransferaseAdd
BLAST
Regioni329 – 687359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4QJM6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLFSPTLSSP LTPSAVKIER ENLKQFELEN FSRYSIFELI ENRCDFYDAL
60 70 80 90 100
LIQLWQEIEL SEQLGIALIA VGGYGRREMF PLSDLDFLIL VEQTPSPEIE
110 120 130 140 150
EKITQFIQFL WDCGFEVGNS VRTLEQCESE GKQDITIATN LLEARFLAGN
160 170 180 190 200
RPHFDALNEL VKRADFWSKE DFFNAKVQEQ IERYQRYHNT AYNLEPDIKY
210 220 230 240 250
SPGGLRDLHL LYWVALRHSG ALTLEAILQS GFIYPQEYQQ LQESRAFLFK
260 270 280 290 300
VRFALHLILK RYDNRLLFDR QIKVSELLGF RGEGNPAVEK MMKCFFQALH
310 320 330 340 350
RISLISNLLI QHYRENVLPS NQDTVIDQLD DDFQLINQCL CLRNSFVFQE
360 370 380 390 400
KPARILDLFF YLTQYEHANI HSDTLRQLQI SLDQLSQKLC EIPAAREKFL
410 420 430 440 450
RLFNQPNAIK RAFMPMHQYG VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT
460 470 480 490 500
LRVMLKLESF LSQESAQEHP IAHRIFSQLS DRTLLYIAAL FHDIAKGRGG
510 520 530 540 550
DHAELGAVDI ADFAQLHGLD RREIDTLAWL VKSHLLMSIT AQRRDIHDPE
560 570 580 590 600
VVMNFAEAVQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTG
610 620 630 640 650
RQFSQGMKEL LDYSEKSAEN RKLAQQILMR DYSDITPISI EQLWARCPED
660 670 680 690 700
YFVRNTPKQI AWHTSLLVDF VEALLVKISN RFSLGGTEVF IYCQDQPHLF
710 720 730 740 750
NKVVSTIGAK KFSIHDAQII TTQDGYVFDS FIITELNGEL VEFDRRRELE
760 770 780 790 800
QALTLALQSE KLPALSIVPN RQLQHFTVQT DVRFLQENKK EHTQMELVAL
810 820 830 840 850
DKAGLLAQVS QIFTELNLNL LNAKITTVGE KAEDFFILTN QFGQALDSQQ
860
REILRNVLYR NIG
Length:863
Mass (Da):100,122
Last modified:July 19, 2005 - v1
Checksum:iA3281DCB51B17273
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000057 Genomic DNA. Translation: AAX88771.1.
RefSeqiYP_249431.1. NC_007146.2.

Genome annotation databases

EnsemblBacteriaiAAX88771; AAX88771; NTHI2026.
GeneIDi3431275.
KEGGihit:NTHI2026.
PATRICi20184074. VBIHaeInf100748_1870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000057 Genomic DNA. Translation: AAX88771.1 .
RefSeqi YP_249431.1. NC_007146.2.

3D structure databases

ProteinModelPortali Q4QJM6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 281310.NTHI2026.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX88771 ; AAX88771 ; NTHI2026 .
GeneIDi 3431275.
KEGGi hit:NTHI2026.
PATRICi 20184074. VBIHaeInf100748_1870.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci HINF281310:GJ89-1871-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
    Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
    J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 86-028NP.

Entry informationi

Entry nameiGLND_HAEI8
AccessioniPrimary (citable) accession number: Q4QJM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 19, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3