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Q4QJM6

- GLND_HAEI8

UniProt

Q4QJM6 - GLND_HAEI8

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Haemophilus influenzae (strain 86-028NP)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciHINF281310:GJ89-1871-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:NTHI2026
    OrganismiHaemophilus influenzae (strain 86-028NP)
    Taxonomic identifieri281310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000002525: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 863863Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192737Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi281310.NTHI2026.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4QJM6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini446 – 579134HDUniRule annotationAdd
    BLAST
    Domaini688 – 77285ACT 1UniRule annotationAdd
    BLAST
    Domaini794 – 86370ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 328328UridylyltransferaseAdd
    BLAST
    Regioni329 – 687359Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4QJM6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLFSPTLSSP LTPSAVKIER ENLKQFELEN FSRYSIFELI ENRCDFYDAL    50
    LIQLWQEIEL SEQLGIALIA VGGYGRREMF PLSDLDFLIL VEQTPSPEIE 100
    EKITQFIQFL WDCGFEVGNS VRTLEQCESE GKQDITIATN LLEARFLAGN 150
    RPHFDALNEL VKRADFWSKE DFFNAKVQEQ IERYQRYHNT AYNLEPDIKY 200
    SPGGLRDLHL LYWVALRHSG ALTLEAILQS GFIYPQEYQQ LQESRAFLFK 250
    VRFALHLILK RYDNRLLFDR QIKVSELLGF RGEGNPAVEK MMKCFFQALH 300
    RISLISNLLI QHYRENVLPS NQDTVIDQLD DDFQLINQCL CLRNSFVFQE 350
    KPARILDLFF YLTQYEHANI HSDTLRQLQI SLDQLSQKLC EIPAAREKFL 400
    RLFNQPNAIK RAFMPMHQYG VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT 450
    LRVMLKLESF LSQESAQEHP IAHRIFSQLS DRTLLYIAAL FHDIAKGRGG 500
    DHAELGAVDI ADFAQLHGLD RREIDTLAWL VKSHLLMSIT AQRRDIHDPE 550
    VVMNFAEAVQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTG 600
    RQFSQGMKEL LDYSEKSAEN RKLAQQILMR DYSDITPISI EQLWARCPED 650
    YFVRNTPKQI AWHTSLLVDF VEALLVKISN RFSLGGTEVF IYCQDQPHLF 700
    NKVVSTIGAK KFSIHDAQII TTQDGYVFDS FIITELNGEL VEFDRRRELE 750
    QALTLALQSE KLPALSIVPN RQLQHFTVQT DVRFLQENKK EHTQMELVAL 800
    DKAGLLAQVS QIFTELNLNL LNAKITTVGE KAEDFFILTN QFGQALDSQQ 850
    REILRNVLYR NIG 863
    Length:863
    Mass (Da):100,122
    Last modified:July 19, 2005 - v1
    Checksum:iA3281DCB51B17273
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX88771.1.
    RefSeqiYP_249431.1. NC_007146.2.

    Genome annotation databases

    EnsemblBacteriaiAAX88771; AAX88771; NTHI2026.
    GeneIDi3431275.
    KEGGihit:NTHI2026.
    PATRICi20184074. VBIHaeInf100748_1870.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000057 Genomic DNA. Translation: AAX88771.1 .
    RefSeqi YP_249431.1. NC_007146.2.

    3D structure databases

    ProteinModelPortali Q4QJM6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 281310.NTHI2026.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX88771 ; AAX88771 ; NTHI2026 .
    GeneIDi 3431275.
    KEGGi hit:NTHI2026.
    PATRICi 20184074. VBIHaeInf100748_1870.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci HINF281310:GJ89-1871-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20."
      Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B., Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O., Munson R.S. Jr.
      J. Bacteriol. 187:4627-4636(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 86-028NP.

    Entry informationi

    Entry nameiGLND_HAEI8
    AccessioniPrimary (citable) accession number: Q4QJM6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3